2007 Fiscal Year Final Research Report Summary
The cloning and investigations for antidepressants affected protein with new chemical proteomics approach
| Project/Area Number |
18591313
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | St.Marianna University School of Medicine |
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Principal Investigator |
OSADA Kenichi St.Marianna University School of Medicine, Department of Neuropsychiatry, Lecturer (20233504)
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| Co-Investigator(Kenkyū-buntansha) |
MATUI Hiroaki St. Marianna University School of Medicine, Radioisotope Research Institute, Professor (90181685)
YAMADA Mituhiko National Institute of Mental Helth, National Center of Neurology and Psychiatry, Director (60240040)
KIDA Satoshi Tokyo University of Agriculture, Faculty of Applied Bioscience, Department of Bioscience, Laboratory of Animal Molecular Biology, Associate Professor (80301547)
KANEKO Rituko Touyou University, Department of Bioscience, Professor (00161183)
KATOU Tomohiro St. Marianna University School of Medicine, Department of Biochemistry, Professor (80233807)
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| Project Period (FY) |
2006 – 2007
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| Keywords | milnacipran / SNRI / SSRI / NBD-F / binding protein |
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| Research Abstract |
Since both preclinical and clinical studies have clearly implicated the serotonin (5-HT) and norepine phrine neurotransmitter systems in the mechanism of action of antidepressants, a monoaminergic hypothesis of the pathophysiology of depression was put forward. Our study investigated how SSRI, SNRI acted in vivo neuron, and which protein bond antidepressants.
We used milnacipran (SNRI) hiaffinity colum and applied rat brain cytosol fraction. Antidepressants binding protein was detected the 2D analysis system variable imageanalyzer, and identify by the mass spectrometry.
Milnacipran bond 4-Fluoro-7-nitro-2, 1, 3- benzoxadiazole (NBD-F) was detected in vivo PC12 cells by confocal microscope. We investigated that milnacipran bond NBD-F colocalize antidepressants binding protein by confocal microscope.
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[Journal Article] The suicidal preventive countermeasure and depression.2006
Author(s)
K. Osada, M. Nakano, A. Misonoo, K. Takahashi, M. Takahashi, H. Hasegawa, S. Kanai, Y. Sasuga, D. Tanaka, N. Watanabe, M. Yamada, M. Asakura
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Journal Title
Journal of Mental Health 19
Pages: 49-58
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The Proteome Analysis to the rat brain protein after long-term lithium2006
Author(s)
M. Nakano, K. Osada, A. Misonoo, K. Takahashi, M. Takahashi, Y. Ogawa, S. Kanai, D. Tanaka, T. Hishinuma, Y. Sasuga, H. Nishikawa, H, Matsui, M. Yamada, M. Asakura
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Journal Title
Neurochemistry 45
Pages: 544
Description
「研究成果報告書概要(欧文)」より
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