2007 Fiscal Year Final Research Report Summary
SCREENING FOR ANTIDEPRESSANT RELATED GENES RELATED TO NEUROGENESIS
| Project/Area Number |
18591323
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Psychiatric science
|
|---|
| Research Institution | National Center of Neurology and Psychiatry |
|---|
Principal Investigator |
YAMADA Mitsuhiko National Center of Neurology and Psychiatry, NATIONAL CENTER OF NEUROLOGY AND PSYCHIATRY, DIVISION OF PSYCHOGERIATRICS, DIRECTOR (60240040)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMADA Misa NATIONAL CENTER OF NEUROLOGY AND PSYCHIATRY, DIVISION OF PSYCHOGERIATRICS, Researcher (10384182)
TAKAHASHI Kou NATIONAL CENTER OF NEUROLOGY AND PSYCHIATRY, DIVISION OF PSYCHOGERIATRICS, Researcher (20415582)
MARUYAMA Yoshiaki NATIONAL CENTER OF NEUROLOGY AND PSYCHIATRY, DIVISION OF PSYCHOGERIATRICS, Researcher (70421831)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Keywords | ANTIDEPRESSANT / ECT / NEUROGENESIS / DIFFERENTIAL CLONING |
|---|
| Research Abstract |
We have previously performed EST analysis to identify some molecular machinery responsible for antidepressant effect. To date, several partial cDNA fragments as antidepressant related genes/ESTs (ADRGs) were isolated from rat brain. Then we developed our original cDNA microarray (ADRG microarray) using these ADRG genes Recently, in experimental models, chronic treatment of antidepressant increased adult hippocampal neurogenesis in robust. ECT that is another therapy particularly used in the treatment of drug-resistant depression, also up-regulated adult. A recent study also demonstrates that irradiation of the hippocampus, which reduces hippocampal neurogenesis, abolishes the behavioral effects of the antidepressant. These observations suggest that hippocampal neurogenesis may play an important role in the therapeutic mechanisms of depression. In the present study, we used ADRG microarray to identify the ADRGs that related to neurogenesis as one of the therapeutic mechanisms of depression. Among the genes, we focused on ADRG116 and ADRG604, both of which were represented rat neuroserpin. Immunohistochemical analysis showed that rat neuroserpin was expressed in the inner of SGZ. Some of the immunoreactive cells were present in hilus and in the area between hilus and SGZ. On the other hand, no immunolabeling cells were expressed in other area of granule cell layer. The expression of rat neuroserpin in the differentiated neuronal stem cells was analyzed by real-time quantitative PCR. 96hr after differentiation, the neuroserpin mRNA expression was significantly increased when compared to the cells before differentiation. In conclusion, our results may support the hypothesis that neuroserpin mediated plastic changes of nervous system and are involved with therapeutic action of antidepressants and ECT.
|
-
[Journal Article] Induction of Neuroserpin Expression in Rat Frontal Cortex after Chronic Antidepressant Treatment and Electroconvulsive Treatment2006
Author(s)
Tanaka, S., Yamada, M, Kitahara, S., Higuchi, T., Honda, K., Kamijima, K and Yamada, M.
-
Journal Title
Jpn. J. Neuropsychopharmacol 26
Pages: 51-56
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
-
[Journal Article] Induction of Neuroserpin Expression m Rat Frontal Cortex after Chronic Antidepressant Treatment and Electroconvulsive Treatment.2006
Author(s)
Tanaka, S., Yamada, M., Kitahara, S., Higuchi, T., Honda, K., Kamijima, K and Yamada, M.
-
Journal Title
Jpn. J. Neuropsychopharmacol. 26
Pages: 51-56
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
