2007 Fiscal Year Final Research Report Summary
Proteomic profiling of autoantibodies in patients with esophageal squamous cell carcinoma
| Project/Area Number |
18591465
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Yamaguchi University |
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Principal Investigator |
TAKEDA Shigeru Yamaguchi University, Graduate School of Medicine, Assistant Professor (50403671)
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| Co-Investigator(Kenkyū-buntansha) |
OKA Masaaki Yamaguchi University, Graduate School of Medicine, Professor (70144946)
TAKASHIMA Motonari Yamaguchi University, Graduate School of Medicine, Assistant Professor (40420525)
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| Project Period (FY) |
2006 – 2007
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| Keywords | proteomics / Esophageal cancer / LC-MS / MS / Tropomyosin |
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| Research Abstract |
To identify proteins associated with esophageal carcinogenesis, we performed protein profiling of 16 esophageal squamous cell carcinomas (ESCCs) and paired non-cancerous tissues by two-dimensional electrophoresis (2-DE) and tandem mass spectrometry. In cancerous tissues, three spots showed significant up-regulation in the amount of protein, while eight spots were significantly down-regulated. The identities of the spots were determined by peptide mass fingerprinting (PMF) with liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) and were confirmed by immunoblotting. The up-regulated proteins were tropomyosin alpha 4 chain, transgelin, and pyruvate kinase. The down-regulated proteins were serum albumin precursor, isoforms of annexin Al, tropomyosin beta chain, 14-3-3 protein sigma and isoforms of serotransferrin precursor. In all 16 cases, up-regulation of the tropomyosin alpha 4 chain was confirmed by immunoblotting. Localization of the tropomyosin alpha 4 chain in ESCC cells and adjacent fibroblasts was confirmed by immunohistochemistry.
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