• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2007 Fiscal Year Final Research Report Summary

Proteomic profiling of autoantibodies in patients with esophageal squamous cell carcinoma

Research Project

Project/Area Number 18591465
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionYamaguchi University

Principal Investigator

TAKEDA Shigeru  Yamaguchi University, Graduate School of Medicine, Assistant Professor (50403671)

Co-Investigator(Kenkyū-buntansha) OKA Masaaki  Yamaguchi University, Graduate School of Medicine, Professor (70144946)
TAKASHIMA Motonari  Yamaguchi University, Graduate School of Medicine, Assistant Professor (40420525)
Project Period (FY) 2006 – 2007
Keywordsproteomics / Esophageal cancer / LC-MS / MS / Tropomyosin
Research Abstract

To identify proteins associated with esophageal carcinogenesis, we performed protein profiling of 16 esophageal squamous cell carcinomas (ESCCs) and paired non-cancerous tissues by two-dimensional electrophoresis (2-DE) and tandem mass spectrometry. In cancerous tissues, three spots showed significant up-regulation in the amount of protein, while eight spots were significantly down-regulated. The identities of the spots were determined by peptide mass fingerprinting (PMF) with liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) and were confirmed by immunoblotting. The up-regulated proteins were tropomyosin alpha 4 chain, transgelin, and pyruvate kinase. The down-regulated proteins were serum albumin precursor, isoforms of annexin Al, tropomyosin beta chain, 14-3-3 protein sigma and isoforms of serotransferrin precursor. In all 16 cases, up-regulation of the tropomyosin alpha 4 chain was confirmed by immunoblotting. Localization of the tropomyosin alpha 4 chain in ESCC cells and adjacent fibroblasts was confirmed by immunohistochemistry.

  • Research Products

    (7 results)

All 2007

All Journal Article (4 results) (of which Peer Reviewed: 2 results) Presentation (3 results)

  • [Journal Article] Expression of tropomyosin alpha 4 chain is increased in esophageal squamous cell carcinoma as evidenced by proteomic profiling by two-dimensional electrophoresis and liquid chromatography-mass spectrometry/mass spectromet.2007

    • Author(s)
      原田 俊夫
    • Journal Title

      Proteomics 1(2)

      Pages: 215-223

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] A clinical study of esophagectomy after chemo-radiation therapy for advanced esophageal carcinoma2007

    • Author(s)
      武田 茂
    • Journal Title

      Gan To Kagaku Ryoho 34(12)

      Pages: 1982-1984

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Expression of tropomyosin alpha 4 chain is increased in esophageal squamous cell carcinoma as evidenced by proteomic profiling by two-dimensional electrophoresis and liquid chromatography-mass spectrometry/mass spectrometry2007

    • Author(s)
      Toshio, Harada
    • Journal Title

      Prpteomics 1(2)

      Pages: 215-223

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A clinical study of esophagectomy after chemo-radiation therapy for advanced esophageal carcinoma2007

    • Author(s)
      Shigeru, Takeda
    • Journal Title

      Gan To Kagaku Ryoho 34(12)

      Pages: 1982-1984

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 進行食道癌における術前化学放射線後食道切除症例の検討2007

    • Author(s)
      武田茂
    • Organizer
      第29回日本癌局所療法研究会
    • Place of Presentation
      ホテル日航東京
    • Year and Date
      2007-06-29
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 食道扁平上皮癌患者における血清蛋白自己抗体の解析2007

    • Author(s)
      原田俊夫
    • Organizer
      第107回日本外科学会学術集会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2007-04-12
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Analysis for Auoantibodies in Sera from esophagus Squamous Cell Carcinoma-Bearing Patients2007

    • Author(s)
      Toshio, Harada
    • Organizer
      The 107th Annual Congress of Japan Surgical Society
    • Place of Presentation
      Osaka
    • Year and Date
      2007-04-12
    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2010-02-04  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi