2007 Fiscal Year Final Research Report Summary
The role of intermediateted filament proteins in hepatic stellate cells in the development of liver fibrosis and hepatocellular carcinoma
| Project/Area Number |
18591513
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
UYAMA Naoki Hyogo College of Medicine, Faculty of Medicine, Professor (70402873)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Jiro Hyogo College of Medicine, Faculty of Medicine, Professor (90199373)
IIMURO Yuji Hyogo College of Medicine, Faculty of Medicine, Associate Professor (30252018)
KAWADA Norifumi Osaka City University, Graduate school of Medine, Faculty of Medicine, Professor (30271191)
IKEDA Kazuo Osaka City University, Graduate school of Medine, Faculty of Medicine, Associate Professor (80275247)
HATANO Etsuro Kyoto University, Graduate school of Medine, Faculty of Medicine, Instructor (80359801)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Intermediate filament / liver fibrois / hepatic stellate cells / hepatocellular carcinoma / synemin / vimentin |
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| Research Abstract |
(A) Expression of Intermediate filament proteins by rat hepatic stellate cells.
Expression of intermediated filament proteins in rat normal or cirrhotic liver tissues was investigated. In normal liver, GFAP, desmin, vimentin and synemin were expressed by hepatic stellate cells. In cirrhotic liver, expression of GFAP and synemin was down-regulated and expression of desmin and vimentin was up-regulated. Isolated stellate cells from rat liver are thought to be activated during the culture on the plastic dish. Expression of GFAP and synemin was down-regulated and expression of desmin and vimentin was up-regulated during the culture. So, we found that quiescent rat stellate cells express synmin and GFAP and activated stellate cells express desmin and vimentin.
(B) Expression of Intermediate filament proteins by human hepatic stellate cells.
Expression of GFAP, synemin, desmin and vimentin in human normal or cirrhotic liver tissues was investigated. In normal liver, vimentin and synemin were expressed by hepatic stellate cells. However, desmin and GFAP were not expressed by hepatic stellate cells. In cirrhotic liver, expression of synemin was down-regulated and expression of vimentin was up-regulated. In LX-2 cells, cell line of stellate cells, Expression of vimentin, but not synemin, detected. So, we found that synmin and vimentin play a key role to regulate the activation of hepatic stellate cells.
(C) The role of vimentin in human hepatic stellate cells.
We investigated the role of vimentin in human hepatic stellate cells. Expression of vimentin in LX-2 cells were down regulated by 85% of control by siRNAs. Depletion of vimentin did not affect the ability to proliferate and the expression of type I collagen. In the future, we will investigate the function of vimentin in human hepatic stellate cells again by other methods.
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[Journal Article] Efficacy and toxicity of transcatheter arterial chemoembolization with Cisplatin suspended in lipiodol for unresectable hepatocellular carcinoma2008
Author(s)
Uyama N, Hatano E, Maetani Y, Isoda H, Shibata T, Taura K, Oe S, Naito M, Yasuchika K, Fujii H, Ikai I, Uemoto S.
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Journal Title
Gan To Kagaku Ryoho 35(5)
Pages: 775-80
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Targeted and regulable expression of transgenes in hepatic stellate cells and myofibroblasts in culture and in vivo using an adenoviral Cre/loxP system to antagonise hepatic fibrosis.2007
Author(s)
Kinoshita K, Iimuro Y, Fujimoto J, Inagaki Y, Namikawa K, Kiyama H, Nakajima Y, Otogawa K, Kawada N, Friedman SL, Ikeda K.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Hepatic stellate cells express synemin, a protein bridging intermediate filaments to focal adhesions.2006
Author(s)
Uyama N, Zhao L, Van Rossen E, Hirako Y, Reynaert H, Adams DH, Xue Z, Li Z, Robson R, Pekny M, Geerts A.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Expression and function of synemin in rat and human hepatic stellate cells.2007
Author(s)
Uyama, N., Reynaert, H., Iimuro, Y., Fujimoto, J., Geerts, A.
Organizer
42nd Congress of the European Society for Surgical Research(ESSR)
Place of Presentation
Rotterdam, The Netherlands
Year and Date
20070523-26
Description
「研究成果報告書概要(欧文)」より
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