2007 Fiscal Year Final Research Report Summary
Development of novel cocktail solutions for cryo-preserving human small hepatocytes and generation of humanoid mouse liver
| Project/Area Number |
18591519
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
MIZUGUCHI Toru Sapporo Medical University, Department of Surgery I, Assistant Professor (30347174)
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| Co-Investigator(Kenkyū-buntansha) |
HIRATA Koichi Sapporo Medical University, Department of Surgery I, Professor (50136959)
KAJI Shinsuke Sapporo Medical University, Department of Surgery I, Researcher (90404623)
SHIBATA Toshihito Sapporo Medical University, Department of Surgery I, Researcher (80404622)
NAGAYAMA Minoru Sapporo Medical University, Department of Surgery I, Instructor (40398326)
MEGURO Makoto Sapporo Medical University, Department of Surgery I, Instructor (50448601)
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| Project Period (FY) |
2006 – 2007
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| Keywords | autoorganoid formation / utilization of biofunction / transplantaion and regenerative medicine / applied animal / regenerative medicine |
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| Research Abstract |
Hepatocyte transplanation (HT) is an attractive therapeutic modality for liver disease as an alternative for liver organ transplantation. Primary fresh hepatocytes (FHs) are the exclusive cell source that has been used for clinical HT. However, the use of FHs is limited due to a shortage of donor cells. Small hepatocytes (SHs) are hepatic progenitor cells, and can be isolated not only from rodents but also from humans. SHs can proliferate in vitro and express liver functions, although conventional hepatocytes lose them within a short period after culture. SH functions in vivo have never been studied. We therefore investigated HT using SHs to evaluate cell engraftment and function compared to HT using FHs.
The donor cell number in the SH group was smaller than that in the FH group at HT. The cell engraftment in the SH group was smaller in the liver and larger in the spleen than in the FH group. The cell engraftment in the liver increased after HT ; however, that in the spleen decreased after HT in both groups. HT using SHs supported the serum albumin level in the NAR experiment as well as that using FH, and albumin mRNA was detectable in the recipients' tissues at 12 weeks after HT.
In conclusion, HT using SHs showed hepatic repopulation similar to that using FHs. This suggests that both SHs and FHs can repopulate the liver as if they were hepatic stem cells. In addition, HT using SHs supported liver functions such as albumin correction at the same level as that using FHs. These observations strongly support the idea that SHs could be an alternative to primary FHs as a novel cell source for future HT.
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[Journal Article] New strategy for the antifibrotic therapy with oral administration of FR260330 (a selective inducible nitric oxide synthase inhibitor) in rat experimental liver cirrhosis2007
Author(s)
Kikuchi, H., Katsuramaki, T., Kukita, K., Taketani, S., Meguro, M., Nagayama, M., Isobe, M., Mizuguchi, T., Hirata, K
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Journal Title
Wound Repair Regen 15 (6)
Pages: 881-8
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Portal blood flow regulates volume recovery of the rat liver after partial hepatectomy : molecular evaluation2006
Author(s)
Nobuoka, T., Mizuguchi, T., Oshima, H., Shibata, T., Kimura, Y., Mitaka, T., Katsuramaki, T., Hirata, K
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Journal Title
Eur Surg Res 38
Pages: 522-32
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Assessment of liver fibrosis by a noninvasive method of transient elastography and biochemical markers2006
Author(s)
Kawamoto, M., Mizuguchi, T., Katsuramaki, T., Nagayama, M., Oshima, H., Kawasaki, H., Nobuoka, T., Kimura, Y., Hirata, K
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Journal Title
World J Gastroeterol 12 (27)
Pages: 4325-30
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] In situ graft-trimming method using polyester vascular prosthesis for inferior vena cava reconstruction after hepatectomy2006
Author(s)
Mizuguchi, T., Katsuramaki, T., Morishita, K., Kawamoto, M., Nobuoka, T., Imamura, M., Kimura, Y., Hirata, K
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Journal Title
Dig Surg 23(1-2)
Pages: 115-8
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Serum lipid and lipoprotein alterations represent recovery of liver function after hepatectomy2006
Author(s)
M., Kawamoto, T., Mizuguchi, M., Nagayama, T., Nobuoka, S., Parker, T., Katsuramaki, K., Hirata
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Journal Title
Liver Int 26
Pages: 203-10
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Liver repopulation and long-term function of rat small hepatocyte transplantation as an alternative cell source for hepatocyte transplantation2006
Author(s)
Shibata, C., Mizuguchi, T., Kikkawa, Y., Nobuoka, T., Oshima, H., Kawasaki, H., Kawamoto, M., Katsuramaki, T., Mitaka, T., Hirata, K
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Journal Title
Liver Transpl 12
Pages: 78-87
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Assessment of nutritional status and prediction of postoperative liver function from serum apolioprotein A-1 levels with hepatectomy2006
Author(s)
Katsuramaki, T., Mizuguchi, T., Kawamoto, M., Yamaguchi, K., Meguro, M., Nagayama, M., Nobuoka, T., Kimura, Y., Furuhata, T., Hirata, K
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Journal Title
World J Surg 30(10)
Pages: 1886-91
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Analysis of the changes pattern of serum apolipoprotein A-1 after hepatectomy2006
Author(s)
Katsuramaki, T., Mizuguchi, T., Nagayama, M., Kimura, Y., Furuhata, T., amaguchi, K., Hata, K., Hirata, F
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Journal Title
Hepatogastroenterology 53 (72)
Pages: 924-7
Description
「研究成果報告書概要(欧文)」より
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[Presentation] A perioperative complication-free course without a high serum hyaluronate level ensures prolonged disease-free survival and overall survival after hepatectomy for hepatocellular carcinoma2007
Author(s)
Toru, Mizuguchi, Tadashi, Katsuramaki, Minoru, Nagayama, Makoto, Meguro, Toshihiko, Nishidate, Takayuki, Nobuoka, Yasutoshi, Kimura, Koji, Yamaguchi, Tomohisa, Furuhata, Koichi, Hirata
Organizer
Seventh Americas Hepatopancreatobiliary Congress
Place of Presentation
Las Vegas, USA
Year and Date
20070819-22
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Advantage of post-operative liver function after major hepatectomy using saline-linked cautery2007
Author(s)
Toru, Mizuguchi, Tadashi, Katsuramaki, Minoru, Nagayama, Makoto, Meguro, Takayuki, Nobuoka, Yasutoshi, Kimura, Koji, Yamaguchi, Tomohisa, Furuhata, Koichi, Hirata
Organizer
First biennial congress of the Asian-Pacific Hepato-Pancreato-Biliary Association
Place of Presentation
Fukuoka, Japan
Year and Date
20070321-23
Description
「研究成果報告書概要(欧文)」より
