2007 Fiscal Year Final Research Report Summary

Study on pathways from pancreatic intraepithelial neoplasia toward invasive pancreatic carcinoma by complimentary genomic hybridization

Research Project

Project/Area Number	18591529
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Digestive surgery
Research Institution	Asahi University (2007) Osaka Medical College (2006)
Principal Investigator	TAKAORI Kyoichi Asahi University, Department of Surgery, Professor (10329485)
Project Period (FY)	2006 – 2007
Keywords	Surgery / Pathology / International collaboration / Cancer
Research Abstract	Pancreatic intraepithelial neoplasia (PanIN) is a microscopic lesion identified in the pancreatic duct system and PanIN is presumed to be a precursor to invasive pancreatic carcinoma. However, the pathways from PaniN toward invasive pancreatic carcinoma have not been thoroughly studied so far. The aim of this study was to investigate the pathways from PanIN toward invasive pancreatic carcinoma by complimentary genomic hybridization. Surgical specimens of the pancreas from five patients with invasive pancreatic carcinoma, three with intraductal papillary mucinous neoplasm, two with gastric cancer were used under guidance of ethical codes. Cellular content of PanIN lesions were retrieved by laser micro-dissection under observation by a microscope (PixCell LCM, Aicturus, USA). DNA was isolated for further analysis. Preliminary experiment for degenerate oligonucleotide primed-PCR and complimentary genomic hybridization was carried out.