Non-synonymous SNPs of DNA damage repair genes in lung cancer patients

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 18591547
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Kobe University
• Principal Investigator: MANIWA Yoshimasa Kobe University, Graduate School of Medicine, Assistant Professor (50362778)
• Co-Investigator(Kenkyū-buntansha): OKITA Yutaka Kobe University, Graduate School of Medicine, Professor (40322193) ; HAYASHI Yoshitake Kobe University, School of Medicine, Professor (50189669)
• Project Period (FY): 2006 – 2007
• Keywords: lung cancer / risk factor / DNA damage repair gene / single nucleotide polymorphism

Research Abstract

In this study, we identified gene alterations in lung cancer patients, not from the perspective of acquired mutations caused by various external insults or damaging agents, but from the perspective of hereditary predisposition. We evaluated all the non-synonymous SNPs in the DNA damage repair genes that are present in the available databases, which should allow us to detect those that are closely related with the development of lung adenocarcinoma. Finally, we will organize the data statistically and systematically to facilitate the prediction of a patient's cancer risk. As a results, we confirmed that nonsynonymous SNPs (XRCCI: Arg194Trp, TDG: Gly199Ser, RAD9: His239Arg, POLδ1: Arg119His) were present significantly more frequently in lung cancer patients than in a control group. Alignment analysis revealed that all of the region that contained the SNPs conserved among vertebrates, and the allele frequency is less than 1 % in a normal control population. These findings suggest that this SNP may cause an alteration in the biological activity of the expressed protein. These SNPs may allow to predict the susceptibility of lung cancer in a healthy person.

Research Products

• [Journal Article] damage sensor protein hRad9-a novel molecular target for lung cancer treatment (2008)
  • Author(s): Yuki, T, Maniwa, Y*, Doi, T, Okada, K, Nishio, W, Hayashi, Y, Okita, Y., DNA
  • Journal Title: Dncol Rep (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Double-layered collagen gel hemisphere for cell invasion assay: Successful visualization and quantification of cell invasion activity. (2007)
  • Author(s): 高田昌彦、、眞庭謙昌
  • Journal Title: Cell commun adhes 15 Pages: 157-167
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Double-layered collagen gel hemisphere for cell invasion assay: Successful visualization and quantification of cell invasion activity (2007)
  • Author(s): Takata, M, Maniwa, Y*, Doi, T, Tanaka, Y, Okada, K, Nishio, W, Ohbayashi, C, Yoshimura, M, Hayashi, Y, Okita, Y
  • Journal Title: Cell common adhes 14 Pages: 157-167
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] DNA damage sensor protein hRad9-a novel molecular target for lung cancer treatment
  • Author(s): 湯木 毅、眞庭謙昌
  • Journal Title: Oncology Reports (印刷中) Pages: 2008
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Presentation] Study of a relationship between DNA damage response proteins and lung cancer (2006)
  • Author(s): Maniwa, V
  • Organizer: terra un seminario, Institute Toscana Tumori
  • Place of Presentation: Florence, Italy
  • Year and Date: 2006-10-03
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] DNA damage sensor蛋白質hRad9の肺癌に対する分子標的としての可能性の検討 (2006)
  • Author(s): 湯木 毅、眞庭謙昌
  • Organizer: 第59回日本胸部外科学会定期学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2006-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Non-synonymous. SNPs of DNA damage repair genes in lung cancer patients (2006)
  • Author(s): Maniwa, Y, et. al.
  • Organizer: 59th Annual Meeting of the Japanese Association for Thoracic Surgery
  • Place of Presentation: Yokohama
  • Year and Date: 2006-10-01
  • Description: 「研究成果報告書概要(欧文)」より