2007 Fiscal Year Final Research Report Summary
Research for bone marrow stromal cells (BMSC) transplantation therapy for injured central nervous system using bio-imaging methods
| Project/Area Number |
18591572
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
YANO Shunsuke Hokkaido University, Hokkaido University Hospital, Assistant professor (20374481)
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| Co-Investigator(Kenkyū-buntansha) |
KURODA Satoshi Hokkaido University, Hokkaido University Hospital, Assistant professor, Lecturer (10301904)
HIDA Kazutoshi Hokkaido University, Graduate school of medicine, Associate professor (10238305)
IWASAKI Yoshinobu Hokkaido University, Graduate school of medicine, Professor (00113522)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Bone marrow stromal cell / Cerebral infarction / Brain injury / Spinal cord injury / Bio-imaging / Transplantation / Axonal regeneration |
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| Research Abstract |
In the first study, we aimed to clarify whether in vitro chemical treatment promotes their neuronal differentiation on the level of gene expression. Mice BMSC (bone marrow stromal cell) were co-cultured with neuronal cells and analyzed the treatment-induced changes in the gene expression profile using microarray and RT-PCR techniques. As a result, the BMSC express the genes for several growth factors including NGF-beta and BDNF, indicating their therapeutic role in protecting the injured central nervous system (published in Brain Res, 2006).
In the next study using autoradiography, we revealed that BMSCs have the potential to support the survival of neurons in the marginal region of cerebral infarction and spinal cord injury and can partly differentiate into neurons, regenerating spinal cord tissue at the site of injury. (Published in J Neurotrauma, 2006 (spinal cord injury), and in J Nucl Med, 2006 (cerebral infarction))
And more, we clarified that SDF-1/CXCR4 system may play a critical role in the survival, proliferation and migration of the transplanted BMSC and contribute to recovery of neurological function. (published in Brain Res, 2007) BMSC may protect and repair the damaged neurons through multiple mechanisms, including transdifferentiation, cell fusion, and production of growth factors. (J Neurosci Res, 2008)
Now, we are studying focused on the axonal regeneration to clarify the mechanism of neurogenesis.
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