2007 Fiscal Year Final Research Report Summary
The research of neuronal regeneration in cerebral infarction model to propose the restructuring neuronal circuit
| Project/Area Number |
18591598
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
MIMURA Toshirou Yokohama City University, Graduate School of Medicine, Research fellow (50336583)
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| Co-Investigator(Kenkyū-buntansha) |
KANNO Hiroshi Yokohama City University, Graduate School of Medicine, Assistant Professor (40244496)
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| Project Period (FY) |
2006 – 2007
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| Keywords | regenerative medicine / brain and nerve impairment / stem cell / berebral infarction / neuronal regeneration / bone marrow stromal cell |
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| Research Abstract |
This study was aimed to differentiate bone marrow stromal cells and neural stem cells into neurons without gene manipulation, and, moreover, to control the regeneration of neuronal circuits by electrical stimulation. We made the hypothesis that electrical stimulation, instead of gene manipulation, enable to promote the bone marrow stromal cell's differentiation into neurons. Although we tested electrical stimulating cultivation in various conditions, morphological changes into neurons could not observed. Next, electrical stimulation against the bone marrow stromal cells within culture medium containing bFGF, CNTF and Forskolin was performed. Although the expansion of neurite-like process was observed, which did not demonstrate the neuronal cell differentiation in immunohistochemistry. Electrical potential did not influence the direction of neurite expansion. Neuronal stem cells were attempted the electrical stimulation in the same way. We could not observe the neuron induced from neurosphere. Poly-L-lysin and laminin coating of the dish promote the neuronal induction from neurosphere and neurite expansion, however, which were not influenced by additional electrical stimulation. We also attempted the electrical stimulation against spinal dorsal root ganglion cells as mature neurons. Poly-L-lysin and laminin coating promoted the neurite expansion. However, velocity and direction of neurite expansion were not influenced by electrical stimulation. These results demonstrate that neurite expansion in vitro essentially needs poly-L-lysin and laminin as a substance for foothold, which suggests that enough substances for foothold connecting continuously between stem cells and native neurons are essential in the injured brain. As a subject in the future, we will investigate whether the electrical stimulation in vivo under such condition influences the velocity and direction of axonal expansion.
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[Presentation] 神経分化を誘導する機能的ペプチドを用いた神経再生療法の開発2006
Author(s)
菅野 洋, 久保 篤彦, 味村 俊郎, 横山 高玲, 山本 勇夫, 前田 和彦, 山崎 吉以, 蓮見 由紀子, 有江 祐子, 松本 大輔, 小林 菜穂子, 吉田 哲彦
Organizer
第44回日本人工臓器学会
Place of Presentation
横浜
Year and Date
2006-11-02
Description
「研究成果報告書概要(和文)」より
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[Presentation] The development of neuro-regenerative therapy by the use of neuro-defferentiative functional peptide2006
Author(s)
A, Kubo, H, Kanno, T, Mimura, T, Yokoyama, I, Yamamoto, et. al.
Organizer
The 44th annual meeting of the society of Japanese artificial organ
Place of Presentation
Yokohama
Year and Date
2006-11-02
Description
「研究成果報告書概要(欧文)」より
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[Presentation] VHL蛋白導入による組織幹細胞の神経分化誘導とその再生医学への応用2006
Author(s)
菅野 洋, 久保 篤彦, 味村 俊郎, 横山 高玲, 山本 勇夫, 前田 和彦, 山崎 吉以, 蓮見 由紀子, 有江 祐子, 松本 大輔, 小林 菜穂子, 吉田 哲彦
Organizer
第65回日本脳神経外科学会総会
Place of Presentation
京都
Year and Date
2006-10-20
Description
「研究成果報告書概要(和文)」より
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[Presentation] The neuronal differentiation of tissue stem cells by the introduction of VHL-protein and its application for the regenerative medicine2006
Author(s)
A, Kubo, H, Kanno, T, Mimura, T, Yokayama, I, Yamamoto, et. al.
Organizer
The 65th annual meeting of the Japanese Society of Neurosurgery
Place of Presentation
Kyoto
Year and Date
2006-10-20
Description
「研究成果報告書概要(欧文)」より
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[Presentation] VHL遺伝子/ペプチド導入組織幹細胞を用いた神経再生療法2006
Author(s)
菅野 洋, 久保 篤彦, 味村 俊郎, 横山 高玲, 山本 勇夫, 前田 和彦, 山崎 吉以, 斉藤 知行, 川中 紀邦, 水木 信久, 長瀬 敬, 松本 大輔, 小林 菜穂子
Organizer
第5回日本再生医療学会
Place of Presentation
岡山
Year and Date
2006-03-08
Description
「研究成果報告書概要(和文)」より
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[Presentation] 皮膚由来幹細胞の未分化維持機構と神経分化誘導2006
Author(s)
久保 篤彦, 菅野 洋, 横山 高玲, 味村 俊郎, 中野 修一, 杉本 直巳, 小林 菜穂子, 吉田 徹彦, 山本 勇夫
Organizer
第5回日本再生医療学会総会
Place of Presentation
岡山
Year and Date
2006-03-08
Description
「研究成果報告書概要(和文)」より
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[Presentation] Neuro-regenerative therapy by VH-Lgene/peptide-induced tissue stem cells2006
Author(s)
H, Kanno, A, Kubo, T, Mimura, T, Yokoyama, I, Yamamoto, et. al.
Organizer
The 5th. annual meeting of.Japanese society for Rgenerative Medicine
Place of Presentation
Okayama
Year and Date
2006-03-08
Description
「研究成果報告書概要(欧文)」より
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