Gene expression and protein synthesis after transient focal ischemia under an enriched environment in rats

2008 Fiscal Year Final Research Report

• Project/Area Number: 18591619
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: YOKOTA Chiaki National Cardiovascular Center Research Institute, 内科脳血管部門, 医長 (80300979)
• Co-Investigator(Kenkyū-buntansha): 久下 裕司 北海道大学, アイソトープ総合研究センター, 教授 (70321958)
• Co-Investigator(Renkei-kenkyūsha): 生野 雄二 聖マリア病院, 脳血管内科, 医師
• Project Period (FY): 2006 – 2008
• Keywords: リハビリテーション / 局所脳虚血 / Enriched environment Brain derived neurotrophic factor

Research Abstract

局所脳虚血ラットを、通常ケージで単独に飼育する群(Standard:S群)と運動器具が備えられた充実環境で飼育する群(Enrich:E群)に分けた。虚血前後と2、4週間後の神経・運動機能、脳における神経受容体機能(ベンゾジアゼピン受容体プローブ(123/125IMZ)使用)、遺伝子、蛋白発現を両群で比較検討した。梗塞面積に両群で差はなかった。E群はS群に比べて、神経・運動機能は有意に改善、神経受容体機能は皮質梗塞周辺部において有意に高かった。梗塞周辺部での遺伝子発現は、E群がS群に比べbrain derived neurotrophic factor (BDNF)遺伝子発現が低下、BDNF蛋白は皮質の神経線維に発現しており、E群がS群に比し有意に発現が低下していた。BDNFは、環境刺激による脳神経機能回復に寄与している可能性がある。

Research Products

• [Journal Article] Synthesis and evaluation of radioiodinated cyclooxygenase-2 inhibitors as potential SPECT tracers for cyclooxygenase-2 expression (2006)
  • Author(s): Kuge Y, Katada Y, Simonaka S, Temma T, Kimura H, Kiyono Y, Yokota C, Minematsu K, Seki K, Tamaki N, Ohkura K, Saji H
  • Journal Title: Nucl Med and Biology 33 Pages: 21-27
• [Presentation] Low levels of plasma soluble receptor for advanced glycation end products are associated with severe neurological deficits at admission in acute stroke (2008)
  • Author(s): Yokota C, Minematsu K, Tomii Y, Naganuma M, Ito A, Nagasawa H, Yamaguchi T
  • Organizer: The 4th Korean-Japanese Joint Stroke Conference
  • Place of Presentation: Fukuoka Japan
  • Year and Date: 20081121-23
• [Presentation] Brainderived neurotrophic factor (BDNF)の発現低下は脳虚血後の環境刺激による神経機能回復に関係する (2008)
  • Author(s): 生野雄二、横田千晶、久下裕司、井上裕康、小亀浩一、木戸慎介、原田晃名、堀田真理子、佐治英郎、峰松一夫
  • Organizer: 第20 回日本脳循環代謝学会総会
  • Place of Presentation: 第20 回日本脳循環代謝学会総会 東京ドームホテル 東京都
  • Year and Date: 20081106-07
• [Presentation] Decrease in brain derived neurotrophic factor expression is associated with functional recovery in an enriched environment after experimental stroke (2008)
  • Author(s): Shono Y, Kuge Y, Yokota C, Harada A, Kido S, Kokame K, Inoue H, Hotta M, Saji H, Minematsu K
  • Organizer: International Stroke Conference 2008
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20080220-22
• [Presentation] Evaluation of the effects of hypertension on cerebral metabolic functions after the onset of stroke in spontaneously hypertensive rats (SHR) (2007)
  • Author(s): Temma T, Kuge Y, Sano K, Obokata N, Kamihashi J, Kawashima H, Magata Y, Saji H
  • Organizer: 54th Annual Meeting of the Society of Nuclear Medicine
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20070600
• [Presentation] Analysis of gene expression related to enrich environment after transient focal brain ischemia in rats (2007)
  • Author(s): Shono Y, Kuge Y, Yokota C, Kido S, Kokame K, Inoue H, Hotta M, Minematsu K, Saji H
  • Organizer: Brain'07 & BrainPET '07
  • Place of Presentation: Osaka, Japan
  • Year and Date: 20070520-24