2007 Fiscal Year Final Research Report Summary
Biological effect of Polarized Light Emitting Diode Irradiation on cell body
| Project/Area Number |
18591624
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Department of Clinical Research, National Hospital Organization Kanazawa Medical Center (2007)
Kanazawa University (2006) |
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Principal Investigator |
IKEDA Kazuo Department of Clinical Research, National Hospital Organization Kanazawa Medical Center, Department of Orthopaedic Surgery, Kanazawa Medical Center Professor (60231130)
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| Co-Investigator(Kenkyū-buntansha) |
TOMITA Katsuro Kanazawa University, Department of Orthopaedic Surgery, Professor (00092792)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Light Emitting Diode / Polarized light / Wound healing / Mitochondria / procollagen / 創傷治癒 / 細胞増殖 |
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| Research Abstract |
Background
I would like to propose a new phototherapy using polarized light from Light Emitting Diode (LED). The purpose of this study is to clarify the effect of polarized LED irradiation on wound healing. Methods
Five groups were classified as control (C), unpolarized (U), linearly polarized (L), right circularly polarized (RC), and left circularly polarized (LC) LED irradiation. In vitro study, fibroblast cell cultures were irradiated, and cellular proliferation was evaluated with a WST-8 assay. In vivo study, full-thickness skin defect of 20mm diameter was created on the dorsal side of rats. The ratio of the residual wound area was measured and expression of type 1 and type 3 procollagen mRNA in granulation tissue was determined by real-time RT-PCR method.
Results The cellular proliferation rates of group RC and L were significantly higher than other groups. The ratio of the residual wound area of group RC and L were significantly reduced than group C and U. Expression of type1procollagen mRNA in group RC was significantly increased about 1.5-fold in comparison to the group C. There were no significant differences for about type 3 procollagen.
Conclusions
The right circularly polarized light and linearly polarized light promoted the process of wound healing by increasing the proliferation of fibroblasts, and the right circularly polarized light increased the expression of type 1 procollagen mRNA. The effectiveness of right circularly polarized light suggests that some optical active material which has a circular dichroic spectrum takes part in a biochemical reaction.
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