Transplantation of opioid-gene-transferred autologous macrophage for the treatment of neurogemc pain

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 18591635
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Ehime University
• Principal Investigator: YAMAMOTO Haruyasu Ehime University, Graduate School of Medicine, Professor (10092446)
• Co-Investigator(Kenkyū-buntansha): OGATA Tadanori Ehime University, Graduate School of Medicine, Senior Assistant Professor (30291503) ; 森野 忠夫 愛媛大学, 医学部附属病院, 講師 (20380248) ; NAKANO Yoko Ehime University, Graduate School of Medicine, Senior Assistant Professor
• Project Period (FY): 2006 – 2007
• Keywords: Pain / macrophage / gene-transfer / electroporation / Sciatic nerve

Research Abstract

To develop a novel analgesic treatment for neuropathic pain, opioid gene-transferred autologous macrophages were injected via lumbar puncture into the neuropathic pain model rats. Chronic constriction was produced in the left side by ligation using polyglucolic acid strings. These animals showed apparent hyperalgesia and allodynia in the left frt. Injection of opioid gene-transferred autologous macrophages effectively counteract the pain sensation induced by the chronic constriction. The opioid gene-transferred macrophages migrated into the gray matter in the lumbar spinal cord and showed proenkephalin mRNA. This method may be a useful technique for the treatment of several neuropathic pain.