2007 Fiscal Year Final Research Report Summary
A study on tumor proguession mechanism involved in prostaglandin metabolic clearance genes, that is consisted of the prostaglandin tranasport PGT and 15 hydroxyprostaglandin dehydrogenase PGDH, and intracellular and extracellular EP receptors
| Project/Area Number |
18591743
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | University of Yamanashi |
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Principal Investigator |
NOZAWA Munehiro University of Yamanashi, Department of research Interdisciplinary Graduate School of Medicine and Engineering, A researcher (90418707)
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| Co-Investigator(Kenkyū-buntansha) |
NOMURA Teruhisa university of yamanashi, hospital, Assistant professor (10252040)
TAKEDA Masayuki University of Yamanashi, Department of research Interdisciplinary Graduate School of Medicine and Engineering, Professor (80197318)
ARAKI Isao University of Yamanashi, Department of research Interdisciplinary Graduate School of Medicine and Engineering, Associate Professor (50252424)
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| Project Period (FY) |
2006 – 2007
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| Keywords | thuman nrostate cancer cell lines / human prostate biopsy specimens / human bladder cancer specimens / human renal cancer specimens / laser capture microdissection / real-time PCR / Prostaglandin / prostaglandin metabolic clearance genes |
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| Research Abstract |
Although a dramatic surge has occurred on studies defining the role of cyclooxygenase COX-2 and the product prostaglandins (PGs) in causation and prevention of cancer, the correlation of PG metabolic clearance pathway with cancer has never been investigated yet. The expression levels of genes for prostaglandin synthesis (COX-1, COX-2), genes for prostaglandin metabolic clearance (the prostaglandin transporter PGT and 15 hydroxyprostaglandin dehydrogenase PGDH) and EP receptors (EP1-EP4) were quantified by means of real-time PCR based on SYBR Green I dye detection in several human prostate cancer cell lines (LNCaP, DU145, and PC3 cells), a human prostate normal epithelial cell line (PrEC), human prostate biopsy specimens, human bladder cancer specimens, and human renal cancer specimens. BPH epithelial cells in prostate biopsy specimens, normal bladder mucosal epithelial cells, and normal tubule cells were captured as a control to each cancer cell from the same specimen using a technique
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of laser capture microdissection. PGT mRNA expression was significantly down-regulated to 20% on the one hand, and PGDH was up-regulated to 258-folds higher on the other in PC3 cells (p< 0.001), LNCaP cultured with dehydrotestosterone (p<0.01 and p<0.05, respectively), compared to those in PrEC. In contrast, PGDH was remarkably down-regulated to 5% in DU145 cell with significant reduction of PGT expression (p<0.001 and p<0.05, respectively). There was the same fashion of the expression of PGT and PGDH as PC3 in prostate biopsy specimens and bladder cancer specimens. COX-1 and COX-2 expressions were not detected in cancer cell lines, while not significant in specimen tissue. The expression profiles of EP receptors were unique to each cancer. CONCLUSIONS: The differential expression of prostaglandin metabolic clearance genes make specific prostaglandin environment, that may be involved in controlling cancer causation, preservation and apoptosis via intracellular or extracellular specific receptors. Less
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[Journal Article] Coordinate Control of Prostaglandin E2 Synthesis and Uptake by Hyperosmolarity in Renal Medullary Interstitial Cells2006
Author(s)
Michael, L., Pucci, Shinichi, Endo, Teruhisa, Nomura, Run, Lu, Cho, Khine, Brenda, S., Chan, Yi, Bao, Victor, L., Schuster
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Journal Title
American Journal of Physiology : Renal Physiology 290(3)
Pages: 641-649
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Expression profiles of prostaglandin E2 receptor subtypes in human bladder mucosa with bladder outlet obstruction2007
Author(s)
Hideki, Kobayashi, Isao, Araki, Hidenori, Zakoji, Du, Shuqi, Norifum, Sawada, Tsutomu, Mochizuki, Mitsuharu, Yoshiyama, Takayuki, Tsuchida, Teruhisa, Nomura, Mizuya, Fukasawa, Yoshio, Takihana, Masayuki, Takeda
Organizer
37th ICS 2007
Place of Presentation
Rotterdam, The Netherlands
Year and Date
20070000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Agonists of transient receptor potential channel al cause hyper-reflexic micturition by affecting bladder afferent activity it rats2007
Author(s)
Du, Shuqi, Isao, Araki, Teruhisa, Nomura, Mitsuharu, Yoshiyama, Masayuki, Takeda
Organizer
AUA 2007 annual meeting
Place of Presentation
Anaheim, USA
Year and Date
20070000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Expression of large conductance, voltage-and ca2+- activated K+ channel in human urinary bladder : identification of subunits and differences between normal and bladder outlet obstruction2006
Author(s)
Hidenori, Zakoji, Isao, Araki, Masanori Beppu, Du, Shuqi, Mitsuharu, Yoshiyama, Yuuki, Mikami, Hideki, Kobayashi, Teruhisa, Nomura, Takayuki, Tsuchida, Mizuya, Fukasawa, Yoshio, Takihana, Masayuk, Takeda
Organizer
AUA 2006 annual meeting
Place of Presentation
Atianta, USA
Year and Date
20060000
Description
「研究成果報告書概要(欧文)」より
