2007 Fiscal Year Final Research Report Summary
Bone-marrow-derived stem cell influenoes the pathogenesis of preeclampsia.
| Project/Area Number |
18591803
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Ehime University |
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Principal Investigator |
MATSUBARA Keiichi Ehime University, University Hospital, Associate Professor (80263937)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Emiko Ehime University, Graduate School of Medicine, Assistant Professor (70363223)
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| Project Period (FY) |
2006 – 2007
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| Keywords | hypertension / cell-tissue / embryogenesis / preeclampsia |
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| Research Abstract |
Female NOD/SCID mice were sublethally irradiated and GFP-transgenic bone marrow-derived cells were transplanted. GFP-positive (bone marrow derived) cells were closely aggregated around the embryo in the uterine endometrial decidua early in pregnancy. In the 3rd weeks of gestation, GFP-positive cells formed capillary tubes like vessels in the placenta. Bone marrow derived cells could be involved in the placental neovascularization.
Secondly, male GFP-transgenic mice and female NOD/SCID mice were mated. In the 2nd weeks of gestation, GFP-positive (fetus origin) cells existed in the kidney. Since GFP-positive cells differentiated into renal tubular epithelium the fetal cells may be at work as stem cell in the maternal kidney. Usually, maternal renal cells express angiotensin receptor subtype 1 (AT 1) dominantly ; however, fetal cells in maternal kidney expressed subtype2 (AT2). Fetal cells in maternal blood could not only differentiate partly into maternal organ but also be involved in the maternal physiology via the crosstalk between AT1 and AT2.
Circulating EPCs in female human peripheral blood was increased from proliferative phase through luteal phase and decreased with the course of pregnancy. The number of circulating EPCs was not changed in preeclampsia ; however, EPCs' proliferation derived from preeclamptic patients was significantly increased compared with normal pregnant women after a week culture. We also demonstrated that VEGF stimulated EPCs' mobilization and sFlt-1, which is increased in the peripheral blood in preeclampsia, inhibited the effect using live imaging system. Given the above-mentioned results circulating EPCs' mobilization could be inhibited by increased some serum factors (sFlt-1, etc) in preeclampsia.
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[Journal Article] Effect of growth factors and endothelial progenitor cell on the placental neovascularization2007
Author(s)
Abe, E, Matsubara, K, Matsubara, Y, Katayarna, T, Kusanagi, Y, Ito, M
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Journal Title
Journal of Japan Society for the Study of Hypertension in Pregnancy 15
Pages: 107-108
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Interaction between endothelial progenitor cells and trophoblasts is involved in the neovascularization in placenta2006
Author(s)
Abe, E, Matsubara, K, Matsubara, Y, Katayama, T, Kusanagi, Y, Ito, M
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Journal Title
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「研究成果報告書概要(欧文)」より
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[Presentation] Nucleated red blood cells derived from umbilical venous blood of pregnancy-induced hypertension patients influence on the endothelial progenitor cell proliferation2007
Author(s)
Matsubara, K, Abe, E, Matsubara, Y, Hyodo, S, Ito, M
Organizer
28th Japan Society for the study of hypertension in pregnancy
Place of Presentation
Matsuyama, Ehime, Japan
Year and Date
2007-10-17
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Interaction between endothelial progenitor cells and growth factors influence on the neovascularization in placenta2007
Author(s)
Abe, E, Matsubara, K, Matsubara, Y, Katayama, T, Kusanagi, Y, Ito, M
Organizer
28th Japan Society for the study of hypertension in pregnancy
Place of Presentation
Matsuyama, Ehime, Japan
Year and Date
2007-10-17
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Fetal cells Influence on the maternal organs2006
Author(s)
Matsubara, K, Okumura, M, Abe, E, Matsubara, Y, Ito, M
Organizer
12th Meeting of International Federation of Placenta Association
Place of Presentation
Kobe, Hyogo, Japan
Year and Date
2006-09-09
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Interaction between endothelial progenitor cells and trophoblasts is involved in the neovascularization in placenta2006
Author(s)
Abe, E, Matsubara, K, Matsubara, Y, Katayama, T, Kusanagi, Y, Ito, M
Organizer
12th Meeting of International Federation Placenta Association
Place of Presentation
Kobe, Hyogo, Japan
Year and Date
2006-09-09
Description
「研究成果報告書概要(欧文)」より
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