2009 Fiscal Year Final Research Report
Development of human ovarian tissue cryopreservation technique using ovaries derived from gender identity disorder patients
| Project/Area Number |
18591811
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Saitama Medical University |
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Principal Investigator |
TAKAI Yasushi Saitama Medical University, 医学部, 准教授 (60323549)
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| Co-Investigator(Renkei-kenkyūsha) |
ISHIHARA Osamu 埼玉医科大学, 医学部, 教授 (70176212)
TAKEDA Satoru 順天堂大学, 医学部, 教授 (20143456)
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| Project Period (FY) |
2006 – 2009
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| Keywords | 卵巣 / 凍結保存法 / 性同一性障害 / アポトーシス |
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| Research Abstract |
Ovarian tissue cryopreservation and transplantation are becoming increasingly important issues for preserving fertility for young women with cancer as shown by recent successes in restoring ovarian activity and even fertility. Vitrification seems to be a promising technology in ovarian tissue cryopreservation, but it is still in an initial stage. Human ovarian tissue was obtained from consenting female-to-male GID (gender indentiry disorder) patients in sex reassignment surgery (SRS). Ovarian cortex tissues were cut into slices with the size of 1×10×10mm, which is suitable for transplantation in clinical situation, and cryopreserved by Cryotissue vitrification protocol. The frozen/thawed human ovarian tissues were xenografted into the ovarian bursa of non-obese diabetic severe combined immunodeficient (NOD-SCID) mice, and at least 1000 viable follicles were observed in each slice two weeks after transplantation.
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[Presentation] ヒト卵巣組織のガラス化凍結保存とマウスへの異種移植.2009
Author(s)
高井泰, 新海愛子, 梅沢優子, 関博之, 鈴木宏志, Aidiresi A, 竹田省, 香川則子, 桑山正成, 井上太綬, 石原理
Organizer
第27回日本受精着床学会総会・学術講演会
Place of Presentation
京都
Year and Date
20090806-20090807
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