2007 Fiscal Year Final Research Report Summary
The effect of isoflavones on endometrium and endometrial cancer
| Project/Area Number |
18591814
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Showa University |
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Principal Investigator |
YANAIHARA Atsushi Showa University, Dep of Obstetrics and Gynecology, Gynecology (20276588)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Endometrium / Isoflavones / Cell growth / Gene profiling |
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| Research Abstract |
Abstract: Our objective of this study is to examination for action of isoflavone on human endometrial cell. We determined the effect of a daidzein-rich isoflavone aglycone mixure (Dr.AglyMax[○!R]) on the growth of hormone-dependent endometrial cancer cell (ISHIKAWA cell) in vitro. ISHIKAWA cells were plated into 96-well micoplates, treated with Dr. AglyMax^TM , and incubated for 24h. Cell proliferation was analyzed by ELISA using specific antibodies. We compared cell proliferation between Dr. AglyMax[○!R]at concentration of 100 μM and a control without Dr. AglyMax[○!R].Total RNA was isolated from cultured cells by the acid guanidine thiocyanate- phenol/chloroform method. We also examined the changes in a total of approximately 47, 000 gene expressions using microarray method. The cell proliferation of a Dr. AglyMax[○!R]treatment group was lower than control group (67%)(p<0.01). A total of 1165 genes were down-regulated and 1114 genes were up-regulated following treatment with 100 μM Dr. AglyMax[○!R].Our study suggest that the daidzein-rich isoflavone aglycones inhibited endometrial cell growth and these genes, which are involved in steroid metabolism and cell cycle, are supposed to be related with inhibition of cancer cell growth.
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Research Products
(7 results)
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[Presentation] Isoflavones regulate secretion of leukemia inhibitory factor and transforming growth factor beta and expression of glycodelin in human endometrial epithelial cells2007
Author(s)
JW., Xu, N., Yasui, K., Ikeda, WJ., Pan, J., Watanabe, A., Yanaihara
Organizer
63rd Annual Meeting American Society for Reproductive MedicineWashington, DC 2007
Year and Date
20070000
Description
「研究成果報告書概要(欧文)」より
