Role of Bacterial Endotoxin and Innate Immune System in Endometriosis

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 18591837
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Nagasaki University
• Principal Investigator: KHAN Khaleque Nagasaki University, Department if Obstetrics and Gynecology, Assistant Professor (60336162)
• Co-Investigator(Kenkyū-buntansha): KITAJIMA Michio Nagasaki University, Dept. of Obstetrics and Gynecology, Assistant Professor (50380845) ; KHAN Khaleque Nagasaki University, Dept. of Obstetrics and Gynecology, Assistant Professor (60336162)
• Project Period (FY): 2006 – 2007
• Keywords: Endometriosis / Bacterial endotoxin / Peritoneal fluid / Menstrual blood / Escherichia coli / PGE2 / Complements C3 / C4

Research Abstract

We demonstrated higher concentration of bacterial endotoxin in the peritoneal fluid and TLR4-mediated growth of endometriosis in our research experiment of 2006. We extended our research experiment in 2007 and examined the source of bacterial endotoxin in menstrual blood and peritoneal fluid of women with endometriosis. Escherichia coli(E.coli, strain K-12, serotype, 0111: B4)was cultured in LB(Laria-Bertani)agar plate and was treated with a variable concentration of PGE2 in culture.
E.coli was detected in menstrual blood and this was significantly higher in women with endometriosis(10^5-10^7CFU/ml)than that in non-endometriosis(<10^2CFU/ml). As a cell wall extract of E.coli, we also detected higher concentration of endotoxin in the menstrual blood of women with endometriosis than that in non-endometriosis. A significantly increased growth of E.coli was observed in response to a wide range of PGE2 concentration(1pg/ml-100ng/ml)when compared with control(DMSO without PGE2).PGE2 level was also significantly higher in the menstrual blood of women with endometriosis than that in control women(negative control)or that in seminal fluid(positive control).Addition of PHA(1μg/ml-100g/ml) dose-dependently and significantly increased the growth of lymphocytes of both women with and without endometriosis. However, co-treatment with PGE2 significantly abrogated PHA-stimulated growth of lymphocytes. This growth suppressive effect of PGE2 on lymphocytes was only observed in women with endometriosis. The co-culture experiment with PHA-treated lymphocytes and culture media derived from either macrophages or stromal cells significantly suppressed the growth promoting effect of PHA and this effect was recovered after treatment with NS-398, a selective COX2 inhibitor. Lower complements C3/C4 levels were also found in the menstrual blood of women with endometriosis that may promote E.coli growth.
Our results suggest that higher PGE2 levels in menstrual blood, a direct growth promoting or immunosuppressive effect of PGE2 and complement system could be responsible for the increased contamination of E.coli in the menstrual blood of women with endometriosis. These may be consequently involved in higher bacterial endotoxin levels in PF and TLR4-mediated growth of endometriosis.

Research Products

• [Journal Article] Multifunctional role of hepatocyte growth factor in the development of pelvic endometriosis (2008)
  • Author(s): Khan KN
  • Journal Title: WES e-journal (in press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Immunopathogenesis of pelvic endometriosis:role of hepatocyte growth factor,macrophages and ovarian steroids (2008)
  • Author(s): Khan KN
  • Journal Title: Am J Reprod Immunol (in press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Toll-like receptor 4(TLR4)-mediated growth of endometriosis by human heat shock protein 70 (2008)
  • Author(s): Khan KN
  • Journal Title: Hum Reprod (in press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Re-evaluation of the indication and limitation of laparoscopic salpingotomy for tubal pregnancy (2008)
  • Author(s): Fujishita A
  • Journal Title: Euro J Obstet Gynecol Reprod Biol 37 Pages: 210-216
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Multifunctional role of hepatocyte growth factor in the development of pelvic endometriosis (2008)
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Fujishita, A., Ishimaru, T., Sekine, I., Masuzaki, H
  • Journal Title: WES e-journal(review article) (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Immunopathogenesis of pelvic endometriosis : role of hepatocyte growth factor, macrophages and ovarian steroids (2008)
  • Author(s): Khan, KN., Ishimaru, T., Kitajima, M., Hiraki, K., Fujishita, A., Sekine, I., Masuzaki, H
  • Journal Title: Am J Reprod Immunol(review article) (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Toll-like receptor 4(TLR4)-mediated growth of endometriosis by human heat shock protein 70 (2008)
  • Author(s): Khan, KN., Kitajima, M., Imamura, T., Hiraki, K., Fujishita, A., Sekine, I., Ishimaru, T., Masuzaki, H
  • Journal Title: Hum Reprod (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Re-evaluation of the indication and limitation of laparoscopic salpingotomy for tubal pregnancy (2008)
  • Author(s): Fujishita, A., Khan, KN., Kitajima, M., Hiraki, K., Miura, S., Ishimaru, T., Masuzaki, H
  • Journal Title: Euro J Obstet Gynecol Reprod Biol 37(2) Pages: 210-216
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 自然免疫におけるToll-like receptors-子宮内膜,子宮内膜症,および胎盤の病理における細菌性エンドトキシンとToll-like receptor(TLR)4の役割 (2007)
  • Author(s): カレク・ネワズ・カーン
  • Journal Title: 炎症と免疫 15 Pages: 56-58
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Higher prostagIandin E2 and lower complements C3/C4 levels in menstrual blood:a possible mechanism of Escherichia coli contamination in the menstrual blood of women with endometriosis (2007)
  • Author(s): Khan KN
  • Journal Title: Am J Reprod Immunol 57 Pages: 323-324
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Role of bacterial endotoxin and endogenous heat shock protein 70(Hsp70)in pelvic inflammation and TLR4-meidated growth of endometriosis (2007)
  • Author(s): Khan KN
  • Journal Title: Am J Reprod Immunol 58 Pages: 198-199
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Higher levels of hepatocyte growth factor in menstrual blood and peritoneal fluid:an association with the development of endometriosis (2007)
  • Author(s): Khan KN
  • Journal Title: Proceedings of the 20th Asian and Oceanic Congress of Obstetrics and Gynecology Pages: 196
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Toll-like receptors in innate immunity : role of bacterial endotoxin and toll-like receptor 4(TLR4)in endometrium, endometriosis and placenta (2007)
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Fujishita, A., Ishimaru, T., Masuzaki, H
  • Journal Title: Inflammation and Immunity(review article in Japanese) 15 Pages: 56-68
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Higher prostaglandin E2 and lower complements C3/C4 levels in menstrual blood : a possible mechanism of Escherichia coli contamination in the menstrual blood of women with endometriosis (2007)
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Fujishita, A., Ishimaru, T., Sekine, I., Masuzaki, H
  • Journal Title: Am J Reprod Immunol 57(5) Pages: 323-324
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Role of bacterial endotoxin and endogenous heat shock protein 70(Hsp70)in pelvic inflammation and TLR4-meidated growth of endometriosis (2007)
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Fujishita, A., Ishimaru, T., Sekine, I., Masuzaki, H
  • Journal Title: Am J Reprod Immunol 58(3) Pages: 198-199
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Higher levels of hepatocyte growth factor in menstrual blood and peritoneal fluid : an association with the development of endometriosis (2007)
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Fujishita, A., Ishimaru, T., Sekine, I., Masuzaki, H
  • Journal Title: Proceedings of the 20th Asian and Oceanic Congress of Obstetrics and Gynecology Pages: 196
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differential infiltration of macrophages and prostaglandin production by different uterine leiomyomas (2006)
  • Author(s): Miura S
  • Journal Title: Hum Reprod 21 Pages: 2545-2554
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Differential infiltration of macrophages and prostaglandin production by different uterine leiomyomas (2006)
  • Author(s): Miura, S., Khan, KN., Kitajima, M., Hiraki, M., Shingo, M., Masuzaki, H., Samejima, T., Fujishita, A., Ishimaru, T
  • Journal Title: Hum Reprod 21(10) Pages: 2545-2554
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Prostaglandin E2 stimulates the growth of Escherichia coli:an association with E.coli contamination in menstrual blood,higher endotoxin level in peritoneal fluid and TLR4-mediated growth of endometriosis (2008)
  • Author(s): Khan KN
  • Organizer: The 10th World Congress of Endometriosis(WCE)
  • Place of Presentation: Melbourne,Australia
  • Year and Date: 20080311-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Heat treatment of human serum promotes the growth of Escherichia coli:apossible involvement of complement system in the growth of endometriosis (2008)
  • Author(s): Khan KN
  • Organizer: The 10th World Congress of Endometriosis(WCE)
  • Place of Presentation: Melbourne,Australia
  • Year and Date: 20080311-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Prostaglandin E2 stimulates the growth of Escherichia coli : an association with E.coli contamination in menstrual blood, higher endotoxin level in peritoneal fluid and TLR4-mediated growth of endometriosis (2008)
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Fujishita, A., Katamine, S., Sekine, I., Ishimaru, T., Masuzaki, H
  • Organizer: Selected oral presentation at the 10th World Congress of Endometriosis(WCE)
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 20080311-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Heat treatment of human serum promotes the growth of Escherichia coli : a possible involvement of complement system in the growth of endometriosis (2008)
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Fujishita, A., Katamine, S., Sekine, I., Ishimaru, T., Masuzaki, H
  • Organizer: Poster presentation at the 10th World Congress of Endometriosis(WCE)
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 20080311-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 子宮内膜症の増殖と精漿との関連:子宮内膜症の発症予防にコンドームは有効か (2008)
  • Author(s): カレクネワズカーン
  • Organizer: 第29回エンドメトリオーシス研究会
  • Place of Presentation: 高知
  • Year and Date: 20080119-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Seminal Fluid Promotes the Growth of Endometriosis : Is Condom Use Necessary to Prevent the Development of Endometriosis? (2008)
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Fujishita, A., Sekine, I., Ishimaru, T., Masuzaki, H
  • Organizer: The 29th Endometriosis Research Meeting
  • Place of Presentation: Kochi
  • Year and Date: 20080119-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 子宮内膜症,子宮腺筋症および子宮筋腫における組織中マクロファージ浸潤と血管密度に対するGnRHアゴニストの影響 (2007)
  • Author(s): カレクネワズカーン
  • Organizer: 第52回日本生殖医学会
  • Place of Presentation: 秋田
  • Year and Date: 20071025-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Higher levels of hepatocyte growth factor In menstrual blood and peritoneal fluid:an Association with the development of pelvic endometriosis (2007)
  • Author(s): Khan KN
  • Organizer: The 20th Annual Meeting of Asian and Oceanic Congress of Obstetrics and Gynecology(AOCOG)
  • Place of Presentation: Tokyo,Japan
  • Year and Date: 20070921-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Higher levels of hepatocyte growth factor In menstrual blood and peritoneal fluid : an Association with the development of pelvic endometriosis (2007)
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Imamura, T., Fujishita, A., Sekine, I., Ishimaru, T., Masuzaki, H
  • Organizer: Oral Presentation at the 20th Annual Meeting of Asian and Oceanic Congress of Obstetrics and Gynecology(AOCOG)
  • Place of Presentation: Tokyo
  • Year and Date: 20070921-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Role of bacterial endotoxin and endogenous heat shock protein 70(Hsp70) in pelvic inflammation and TLR4-mediated growth of endometriosis (2007)
  • Author(s): Khan KN
  • Organizer: The 5th European Congress of Reproductive Immunology(ECRI)
  • Place of Presentation: Berlin,Germany
  • Year and Date: 20070830-0902
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Role of bacterial endotoxin and endogenous heat shock protein 70(Hsp70)in pelvic inflammation and TLR4-mediated growth of endometriosis (2007)
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Imamura, T., Fujishita, A., Sekine, I., Ishimaru, T., Masuzaki, H
  • Organizer: Oral presented at the seminar session of The 5th European Congress of Reproductive Immunology(ECRI)
  • Place of Presentation: Berlin, Germany
  • Year and Date: 20070830-0902
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Higher Prostaglandin E2 and lower complements C3/C4 levels in menstrual blood : a possible mechanismof Escherichia coli contamination in the menstrual blood of women with endometriosis (2007)
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Imamura, T., Fujishita, A., Sekine, I., Ishimaru, T., Masuzaki, H
  • Organizer: The 59th Japan Obstetrics & Gynecology Congress
  • Place of Presentation: Kyoto
  • Year and Date: 20070814-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Higher Prostaglandin E2 and lower complements C3/C4levels in menstrual blood:apossible mechanism of Escherichia coli contamination in the menstrual blood of women with endometriosis (2007)
  • Author(s): Khan KN
  • Organizer: The 27th Annual Meeting of American Society for Reproductive Immunology(ASRI)
  • Place of Presentation: Toronto,Canada
  • Year and Date: 20070514-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Higher Prostaglandin E2 and lower complements C3/C4 levels in menstrual blood : a possible mechanism of Escherichia coli contamination in the menstrual blood of women with endometriosis (2007)
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Imamura, T., Fujishita, A., Sekine, I., Ishimaru, T., Masuzaki, H
  • Organizer: Oral presentation at the 27th Annual Meeting of American Society for Reproductive Immunology(ASRI)in Toronto
  • Place of Presentation: Canada
  • Year and Date: 20070514-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 月経血中のプロスタグランジンE2濃度の上昇と補体C3/C4濃度の低下は子宮内膜症患者の月経血中への大腸菌(Escherichia Coli)の混入に関与する (2007)
  • Author(s): カレクネワズカーン
  • Organizer: 第59回日本産科婦人科学会
  • Place of Presentation: 京都
  • Year and Date: 20070414-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Differential infiltration of macrophages and prostaglandin production by different uterine leiomyomas:a possible implication in myoma-associated infertility (2007)
  • Author(s): Khan KN
  • Organizer: The 16th Annual Congress of International Society for Gynecologic Endoscopy(ISGE)
  • Place of Presentation: Osaka,Japan
  • Year and Date: 20070318-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Differential infiltration of macrophages and prostaglandin production by different uterine Ieiomyomas : a possible implication in myoma-associated infertility (2007)
  • Author(s): Khan, KN., Miura, S., Kitajima, M., Hiraki, K., Moriyama, S., Samejima, T., Fujishita, A., Sekine, I., Ishimaru, T., Masuzaki, H
  • Organizer: Oral presentation at the 16th Annual Congress of International Society for Gynecologic Endoscopy(ISGE)
  • Place of Presentation: Osaka
  • Year and Date: 20070318-21
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ヒト血清の加熱処理によりEscherichia Coliの増殖が続伸される:子宮内膜症の増殖における補体の関与に関する基礎的検討 (2007)
  • Author(s): カレクネワズカーン
  • Organizer: 第28回エンドメトリオーシス研究会
  • Place of Presentation: 和歌山
  • Year and Date: 20070119-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] プロスタグランジンE2(PGE2)はEscherichia Coliの増殖を刺激する:月経血中のEscherichia ColiとPGE2との関連についての基礎的検討 (2007)
  • Author(s): カレクネワズカーン
  • Organizer: 第28回エンドメトリオーシス研究会
  • Place of Presentation: 和歌山
  • Year and Date: 20070119-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 子宮内膜症腹膜病変の活動性と骨盤内炎症 (2007)
  • Author(s): カレクネワズカーン
  • Organizer: 第5回子宮内膜疾患研究会
  • Place of Presentation: 東京
  • Year and Date: 2007-05-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Activity of pelvic endometriosis and its relationship with pelvic inflammation (2007)
  • Author(s): Khan, KN., Kitajima, M., Sekine, I., Ishimaru, I., Masuzaki, H
  • Organizer: Paper presented at the Symposium session of 5th Endometriosis Symposium
  • Year and Date: 2007-05-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Escherichia coli in menstrual blood:an association with bacterial endotoxin and toll-like receptor 4 (TLR4)-mediated growth of endometdosis (2006)
  • Author(s): Khan KN
  • Organizer: 3rd International Congress on Reproductive Immunobiology
  • Place of Presentation: Banff,Canada
  • Year and Date: 20061115-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Escherichia coli in menstrual blood : an association with bacterial endotoxin and toll-like receptor 4(TLR4)-mediated growth of endometriosis (2006)
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Fujishita, A., Ishimaru, T., Masuzaki, H
  • Organizer: Paper presentation at the Symposium session of 3rd International Congress on Reproductive Immunobiology
  • Place of Presentation: Banff, Canada
  • Year and Date: 20061115-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Bacterial endotoxin, toll-like receptor 4(TLR4)and macrophages : an association in the pathogenesis of endometriosis (2006)
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Moriyama, S., Yamaguchi, N., Katamine, S., Matsuyama, T., Sekine, I., Fujishita, A., Ishimaru, T., Masuzaki, H
  • Organizer: Paper presentation at Symposium session of the 51st Annual Meeting of the Japan Society for Reproductive Medicine(JSRM)
  • Place of Presentation: in Osaka
  • Year and Date: 20061109-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 子宮内膜症の病態に及ぼすエンドトキシン,Toll-like Receptor4(TLR4)およびマクロファージの影響 (2006)
  • Author(s): カレクネワズカーン
  • Organizer: 第51日本生殖医学会
  • Place of Presentation: 大阪
  • Year and Date: 20061108-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Toll-like receptor 4-mediated growth of endometriosis by endogenous heat shock protein 70 (2006)
  • Author(s): Khan KN
  • Organizer: Endo2006, The 88th Annual Meeting of American Endocrine Society
  • Place of Presentation: Boston,USA
  • Year and Date: 20060624-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Toll-like receptor 4-mediated growth of endometriosis by endogenous heat shock protein 70 (2006)
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Masuzaki, H., Matsuyama, T., Sekine, I., Fujishita, A., Ishimaru, T
  • Organizer: Endo2006, The 88th Annual Meeting of American Endocrine Society
  • Place of Presentation: Boston, USA
  • Year and Date: 20060624-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 月経血中の大腸菌(Escherichia Coli)および腹水中のエンドトキシンは骨盤内炎症およびToll-like receptor4を介した子宮内膜症の増殖に関与する (2006)
  • Author(s): カレクネワズカーン
  • Organizer: 第58回日本産科婦人科学会
  • Place of Presentation: 横浜
  • Year and Date: 20060422-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 子宮内膜間質細胞におけるinterleukin-6およびtumor necrosis factor αを介したhepatocyte growth factorの発現調節とそれらの子宮内膜症増殖への関与 (2006)
  • Author(s): 今村 健仁
  • Organizer: 第58回日本産科婦人科学会
  • Place of Presentation: 横浜
  • Year and Date: 20060422-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Changes in macrophage infiltration and micro-vessel density in endometriosis, adenomyosis and uterine myoma after GnRH agonist therapy
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Imamura, T., Fujishita, A., Sekine, I., Ishimaru, T., Masuzaki, H
  • Organizer: The 52nd Annual Meeting of Japan Society for Reproductive Medicine(JSRM)
  • Place of Presentation: Akita
  • Year and Date: 00001125-26
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Escherichia coli in menstrual blood : an association with bacterial endotoxin and toll-like receptor 4(TLR4)mediated growth of endometriosis
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Masuzaki, H., Matsuyama, T., Sekine, I., Fujishita, A., Ishimaru, T
  • Organizer: The 58th Japan Obstetrics & Gynecology Congress
  • Place of Presentation: in Yokohama
  • Year and Date: 00000422-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Interleukin-6 and tumor necrosis factor α-mediated expression of hepatocyte growth factor and its involvement in the growth of endometriosis
  • Author(s): Imamura, T., Khan, KN., Kitajima, M., Hiraki, K., Masuzaki, H., Matsuyama, T., Sekine, I., Fujishita, A., Ishimaru, T
  • Organizer: The 58th Japan Obstetrics & Gynecology Congress
  • Place of Presentation: in Yokohama
  • Year and Date: 00000422-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Heat treatment of human serum promotes the growth of Escherichia coli : a possible involvement of complement system in the growth of endometriosis
  • Author(s): Khan, KN., Kitajima, M., Hiraki, K., Imamura, T., Fujishita, A., Sekine, I., Ishimaru, T., Masuzaki, H
  • Organizer: The 28th Endometriosis Research Meeting
  • Place of Presentation: Wakayama
  • Year and Date: 00000119-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Prostaglandin E2 stimulates the growth of Escherichia coli : an association between PGE2 and E.coli contamination in the menstrual blood of women with endometriosis
  • Author(s): Khan, KN., Imamura, T., Kitajima, M., Hiraki, K., Fujishita, A., Sekine, I., Ishimaru, T., Masuzaki, H
  • Organizer: The 28th Endometriosis Research Meeting
  • Place of Presentation: Wakayama
  • Year and Date: 00000119-20
  • Description: 「研究成果報告書概要(欧文)」より