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2007 Fiscal Year Final Research Report Summary

Molecular analysis of effects of gtucocorticoids on eosinophilic rhinosinusitis with reference to regulation of inflammatory transcription factors.

Research Project

Project/Area Number 18591868
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionHiroshima University

Principal Investigator

TAKENO Sachio  Hiroshima University, Graduate School of Biomedical Sciences, Associate Professor (50243556)

Project Period (FY) 2006 – 2007
Keywordseosinophils / sinusitis / steroids / glucocorticoid receptor (GR) / transcription factors / toll-like receptor (TLR) / nuclear factor-kappa B / cytokines
Research Abstract

Recent studies have implicated eosinophils as a central player in the persistent inflammation occurring in non-infectious chronic sinusitis. The mechanisms by which eosinophilic inflammation damages the epithelium and contributes to recurrent acute exacerbations in the disease have not been fully elucidated. In the present project, we have investigated molecular mechanisms of effectiveness of nasal steroids in relation to intracellular regulation by transcription factors.
1) We examined cytokine expression and the distribution of glucocorticoid receptor (GR) -positive cells in the sinus muffin The patients with high eosinophilia showed higher density of GR type β distribution in the sinus mucosa. The tendency was dominant especially in infiltrating inflammatory cells in the ethmoid sinus mucosa. The use of nasal steroids effectively attenuated Th2 cytokine expression.
2)We examined transcription factor NF-kB activation and concomitant cytokine expression triggered by LPS and PGN stimulus … More in cultured sinus epithelial cells. The results clearly demonstrate an increase of NF-kB p50 activity after the stimulation in a dose-dependent manner. Pretreatment with glucocorticoids (GCs) effectively inhibited the p50 activation. Immunocytochemical analysis confirmed cellular translocation of p50 subunits into the nucleus with concomitant expression of glucocorticoid receptors and toll-like receptors (TLRs) 2 and 4 in culture cells. These results elucidate the potent anti-inflammatory effects of GCs and inflammatory signal pathways induced though TLRs in paranasal sinus epithelial cells.
3)We assessed whether topical instillation of beclomethasone dipropionate dry powder onto the paranasal sinus improved the clinical and radiological conditions after appropriate surgical intervention. Improvement in the endoscopic appearance scores was obtained in 91.4% of patients and the result was better than that from the previous study (71.7%). Significant decreases in the avenged CT scores were also noted from 5.62 to 1.93 after treatment. We consider that this novel technique is effective for the post-surgical treatment of the disease through the control of the inflammatory process that persists in the sinus cavity. Less

  • Research Products

    (25 results)

All 2007 2006

All Journal Article (20 results) (of which Peer Reviewed: 3 results) Presentation (5 results)

  • [Journal Article] 小児アレルギー性鼻炎と合併症-副鼻腔炎との関連性を中心に-。 特集: 小児とアレルギー性鼻炎。2007

    • Author(s)
      竹野幸夫
    • Journal Title

      JOHNS 23

      Pages: 11-14

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Submucous turbinectomy combined with posterior nasal neurectomy in the management of severe allergic rhinitis: Clinical outcomes and local cytokine changes.2007

    • Author(s)
      Ogawa T, Takeno S, et. al.
    • Journal Title

      Auris Nasus Larynx 34

      Pages: 319-326

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Vitamin D3, vitamin K2, and warfarin regulate bone metabolism in human paranasal sinus bones.2007

    • Author(s)
      Sugimoto I, Takeno S, et. al.
    • Journal Title

      Rhinology 45

      Pages: 208-213

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] アレルギー性鼻炎に対する手術療法のEBM。 特集: アレルギー性鼻炎治療のEBM2007

    • Author(s)
      竹野幸夫
    • Journal Title

      アレルギーの臨床 27

      Pages: 527-532

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 好酸球性副鼻腔炎に対する周術期の局所ステロイド噴霧療法の臨床効果。2007

    • Author(s)
      竹野幸夫、他
    • Journal Title

      日本鼻科学会誌 46

      Pages: 102-108

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Submucous turbinectomy combined with posterior nasal neurectomy in the management of severe allergic rhinitis : Clinical outcomes and local cytokine changes2007

    • Author(s)
      Ogawa, T., Takeno, S., et. al.
    • Journal Title

      Auris Nasus Larynx 34

      Pages: 319-326

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Vitamin D3, vitamin K2, and warfarin regulate bone metabolism in human paranasal sinus bones2007

    • Author(s)
      Sugimoto, I., Takeno, S., et. al.
    • Journal Title

      Rhinology 45

      Pages: 208-213

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Allergic rhinitis in children ; Relationship between paranasal sinusitis2007

    • Author(s)
      Takeno, S
    • Journal Title

      JOHNS 23

      Pages: 11-14

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] EBM of surgical procedures in allergic rhinitis2007

    • Author(s)
      Takeno, S
    • Journal Title

      Allergy no rinsho 27

      Pages: 527-532

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Clinical effectiveness of local application of beclomethasone dipropionate dry powder for the treatment of chronic rhinosinusitis with eosinophil infiltration2007

    • Author(s)
      Takeno, S., et. al.
    • Journal Title

      Japanese Journal of Rhinology 46

      Pages: 102-108

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 特集: 病態に基づく副鼻腔炎の治療戦略。 1.副鼻腔炎の病態の変遷。2006

    • Author(s)
      竹野幸夫、夜陣紘治
    • Journal Title

      JOHNS 22

      Pages: 5-9

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 7.いわゆる好酸球性副鼻腔炎の診断と治療をどのようにするか? (組織学的所見の立場から) 特集: 鼻科診療における論点。2006

    • Author(s)
      竹野幸夫、竹田和正、西 康行
    • Journal Title

      JOHNS 22

      Pages: 1467-1472

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 3)副鼻腔炎病態とケモカイン。 II. 耳鼻咽喉科 特集 ケモカインとアレルギー。2006

    • Author(s)
      竹野幸夫
    • Journal Title

      アレルギー・免疫 13

      Pages: 46-53

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] LPS、PGNの培養副鼻腔粘膜上皮おけるNF-κBの活性化に及ぼす影響。2006

    • Author(s)
      西 康行、竹野幸夫、他
    • Journal Title

      耳鼻咽喉科展望 49

      Pages: 204-207

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 当科における内視鏡下副鼻腔手術の治療成績-アレルギーの関与を中心に-。2006

    • Author(s)
      宮里麻鈴、竹野幸夫、他
    • Journal Title

      耳鼻臨床 補117

      Pages: 60-65

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Etiological changes in pathology of paranasal sinusitis2006

    • Author(s)
      Takeno, S., YaJin, K
    • Journal Title

      JOHNS 22

      Pages: 5-9

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 7. How to diagnose and manage eosinophilic sinusitis from histopathological aspects2006

    • Author(s)
      Takeno, S., Takeda, K., Nishi, Y
    • Journal Title

      JOHNS 22

      Pages: 1467-1472

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Chemokines in paranasal sinusitis2006

    • Author(s)
      Takeno, S
    • Journal Title

      Allergy・Immunology (Japan) 13

      Pages: 46-53

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effects of LPS and PGN on NF-kB activation in cultured paranasal sinus epithelial cells2006

    • Author(s)
      Nishi, Y., Takeno, S., et. al.
    • Journal Title

      Oto-Rhino-Laryngology, Tokyo 49

      Pages: 204-207

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Clinical study on therapeutic outcomes after endoscopic sinus surgery2006

    • Author(s)
      Miyasato, M., Takeno, S., et. al.
    • Journal Title

      Practica Oto-Rhino-Laryngologica Suppl 117

      Pages: 60-65

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Educational Lecture "Functional roles of nitric oxide in the inflammatory responses in human nose and paranasal sinuses."2007

    • Author(s)
      Takeno S.
    • Organizer
      Kyung Hee International Rhinologic Symposium (KIRS) 2007
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      20071109-11
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 気管支喘息と副鼻腔炎に関する実態調査(内科でのアンケート調査)。2007

    • Author(s)
      石野岳志、竹野幸夫、他
    • Organizer
      第25回日本耳鼻咽喉科免疫アレルギー学会
    • Place of Presentation
      甲府
    • Year and Date
      20070329-31
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] ランチョンセミナー「慢性鼻副鼻腔炎の難治化因子とその対応」2007

    • Author(s)
      竹野幸夫
    • Organizer
      第46回日本鼻科学会
    • Place of Presentation
      宇都宮市
    • Year and Date
      2007-09-29
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Luncheon Seminar "Multifactorial processes and therapeutic strategies in the development and persistence of chronic sinusitis."2007

    • Author(s)
      Takeno, S
    • Organizer
      466 Annual Meeting of Japanese Society of Rhinology
    • Place of Presentation
      Utsunomiya, Japan
    • Year and Date
      2007-09-29
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 気管支喘息患者における副鼻腔炎の有病率についての実態調査。2006

    • Author(s)
      竹野幸夫、他
    • Organizer
      第45回日本鼻科学会
    • Place of Presentation
      四日市
    • Year and Date
      20060921-23
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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