• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2007 Fiscal Year Final Research Report Summary

Identification of inner ear specific protein binds to connexin 26

Research Project

Project/Area Number 18591876
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionSapporo Medical University

Principal Investigator

KOKAI Yasuo  Sapporo Medical University, School of Medicine, Professor (20178239)

Co-Investigator(Kenkyū-buntansha) HIMI Tetsuo  Sapporo Medical University, School of Medicine, Professor (90181114)
INAGAKI Masumi  National institue for psychology and neuroscience, 国立精神・神経センター, head (70203198)
Project Period (FY) 2006 – 2007
Keywordsinner ear / proteome / impaired hearing / neuroscinece / biological molecule
Research Abstract

Connexin 26 has been reported to play an important role for hearing impairment, though its molecular mechanism is still poorly understood. To study a role of connexin 26 in hearing impairment, we developed transgenic mice expressing human connexin 26. In these transgenic mice, human connexin26 was expressed all the organs examined, suggesting universal expression of connexin 26. Protein extracts were prepared from inner ear, liver, small intestine, kidney and spleen. Anti-FLAG antibody was used to pull down proteins bound to connexin 26. Bound proteins were analyzed by time of flight type mass spectrometry to make protein profile in these bound proteins. Comparing bound proteins among these organs, several proteins were only detedted in extracts from inner ear. In addition to ear specific proteins, we also detected each organ specific proteins. We are currently purifying these proteins to identify amino acid sequences. Collectively, this approach is found to be very effective to identify organ specific binding proteins to theoretically any type of proteins.

  • Research Products

    (12 results)

All 2008 2007 2006

All Journal Article (11 results) (of which Peer Reviewed: 5 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] CCL8 is a potenial molecular candidate for the diaganosis of graft-vers us-host disease2008

    • Author(s)
      Hori T, et. al.
    • Journal Title

      Blood 111

      Pages: 4403-4412

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] CCL8 is a potential molecular candidate for the diagnosis of graft-versus-host disease2008

    • Author(s)
      Hori, T., et. al.
    • Journal Title

      Blood 111

      Pages: 4403-4412

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Anti-tumor effects of the glycolipids from spinach which inhibited DNA polymerase2007

    • Author(s)
      Maeda N, et. al.
    • Journal Title

      Nutri Cancer 57(2)

      Pages: 216-222

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] 血漿のプロテオミクス2007

    • Author(s)
      苗代 康可, ら
    • Journal Title

      日本臨床免疫学会誌 30(3)

      Pages: 145-150

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Anti-tumor effects of the glycolipids from spinach which inhibited DNA polymerase2007

    • Author(s)
      Maeda, N., et. al.
    • Journal Title

      Nutri Cancer 57(2)

      Pages: 216-222

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Anti-tumor effects of dehydrtenusin, a specific inhibitor for mammalian DNA polymerase a2007

    • Author(s)
      Maeda, N., et. al.
    • Journal Title

      Biochem Biopys Res Comm 352

      Pages: 390-396

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Plasma proteomics2007

    • Author(s)
      Naishiro, Y., et. al.
    • Journal Title

      Jpn, J Clin Immunol 30(3)

      Pages: 145-150

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] リポソームをリガンドとして用いた血漿蛋白質のプロテオミクス2006

    • Author(s)
      木村 成寿, ら
    • Journal Title

      生物物理化学 50

      Pages: 231-236

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Elevation of plasma level of Annexin A5 in Alzheimer's disease2006

    • Author(s)
      Sohma H, et. al.
    • Journal Title

      Advances in Alzheimer's diasease 1

      Pages: 145-151

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Plasma proteomics using liposome as a probe2006

    • Author(s)
      Kimura, M., et. al.
    • Journal Title

      Sibutsu Butsuri Kagaku 50

      Pages: 231-236

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Elevation fo Annexin A5 in Alzheimer's disease2006

    • Author(s)
      Sohma, H., et. al.
    • Journal Title

      Advances in Alzheimer's disease 1

      Pages: 145-151

    • Description
      「研究成果報告書概要(欧文)」より
  • [Patent(Industrial Property Rights)] 移植片対宿主疾患の検査法2007

    • Inventor(s)
      堀司ら
    • Industrial Property Rights Holder
      札医大
    • Industrial Property Number
      特願2007-165547
    • Filing Date
      2007-06-22
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2010-02-04  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi