2007 Fiscal Year Final Research Report Summary
Examination of P27KIP1 mouse retina and neurosphere from the retina
| Project/Area Number |
18591907
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Tohoku University |
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Principal Investigator |
ISHIKAWA Yumi Tohoku University, Tohoku University, graduate school of medicine, technical assistant (60396439)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Toshiaki Tohoku University, graduate school of medicine, Professor (90191858)
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| Project Period (FY) |
2006 – 2007
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| Keywords | p27 / KO ouse / neurosphere / retina / cell ransplantation |
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| Research Abstract |
G0-G1 cell cycle was absent in the mouse of p27^<kiP1> KO (p27(-/-)) mouse. We hypothesized that if we generate retinal degeneration, the restoration may be different from that of wild one and some of the neural regeneration may be happened. We also hypothesized that retinal neurosphere from the p27(-/-)) mouse eye may induce retinal neurons easier than those of other neurospheres. We can transplant the cell subretinally to the retinal degeneration mouse or rat model after making neurosphere and can examine if they changed to the retinal neurons instead of damaged retinal neurons.
We first examined the mouse retina histologically and electrophsiologically. Thicker retina was observed in the p27(-/-) mouse when compared to those of p27(-/+) or wild type mouse. The diameter of the optic nerve was also thicker than those of others. Fundus photograph also showed enlargement of the size of the optic nerve head and the size of the retinal vessels around the optic nerve head. These differences were obvious if the mouse getting older and older. When we examined the histology of the retina after N-methy-N-nitrosourea (MNU) addition, we found that statistically significant thinning of the retina when compared to that of age-matched wild type control. When we examined BrdU incorporation to the retina, we found that there were no difference between p27(-/-) and wild type mice. We also found that nestin positive cells were also present at almost same position of the BrdU positive cells. We tried to make neurospheres from peripheral retina, marginal zone, and iris by the methods reported previously. Some of these cells make neurospheres and showed positivity of nestin, a marker of the neuron. We stained these cells by DiI and transplanted subretinally to the normal or damaged rat (by continuous light damage). As far as we examined by histology, we could not find and DiI positive cells so far. These transplanation are on-going in our laboratory.
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[Journal Article] Recombinant AAV-transduced irispigment epithelial cell transplantation may transfer vector to native RPE but suppress systemic dissemination2006
Author(s)
Yoshioka Y, Abe T, Wakusawa R, Moriya T, Mochizuki S, Saigo Y, Saito T, MurataH, Tokita Y.Iseva T, Sugano E, Ibmita H, Sagara Y, Tamai M
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Journal Title
Invest Ophthalmol Vis Sci. 47
Pages: 745-752
Description
「研究成果報告書概要(欧文)」より
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