2007 Fiscal Year Final Research Report Summary
The development of new retinal oxidative stress market and the effects of vitamin E intensive care against retinal light damage
| Project/Area Number |
18591921
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Shimane University |
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Principal Investigator |
OHIRA Akihiro Shimane University, School of Medicine, Professor (00169054)
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| Co-Investigator(Kenkyū-buntansha) |
KODAMA Tatsuo Shimane University, School of Medicine, Associate Professor (60215283)
TANITO Masaki Shimane University, School of Medicine, Associate Professor (30284037)
KAIZU Sachiko Shimane University, School of Medicine, Assistant Professor (00325052)
NONOYAMA Shin Shimane University, School of Medicine, Assistant Professor (30362924)
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| Project Period (FY) |
2006 – 2007
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| Keywords | hydroxyoctadecadienoic acid(HODE) / 8-iso-prostaglandin F2α / α-Tocopherol / α-Tocotrienol / α-Tocopherol transfer protein (α-TTP) (6) |
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| Research Abstract |
Maternal animals and pups of α-Tocopherol transfer protein (α-TTP) null mice were fed a vitamin E (VE) deficient diet for 4- or 18-months [VE (-) group]. Wild type. C57BL/6 mice were fed with 0.002 % α-tocopherol-supplemented diet [VE (+) group]. In retinas from 4-month-old VE (-) animals, the level of α-tocopherol, as measured by high performance liquid chromatography, was 71-fold lower than VE (+) group and γ-tocopherol was not detected, thus VE (-) group yielded more severe VE deficiency than ever reported. By gas chromatography analysis, n-3 pulyunsaturated fatty acids decreased (0.3- to 0.9-fold) in VE (-) mice, whereas other classes of fatty acids were unchanged or increased. When 18-month-old animals were compared to 4-month-old animals, levels of hydroxyoctadecadienoic acid and 8-iso-prostaglandin F^<2α>, biomarkers for lipid peroxidation, assessed by gas chromatography-mass spectrometry (GC-MS) were increased in retina and retinal pigment epithelium (RPE) from both VE (-) and (+) groups, whereas these age-related increases were more remarkable in VE (-) groups (2.6- to 43.5-fold) than VE (+) groups (0.8- to 8.7-fold). At 18-months of age, by light microscopy, number of outer nuclear layer (ONL) nuclei was 17 % lower in VE (-) than (+) groups (p<0.05). By electron microscopy, larger amounts of fibrous tissues between ONL nuclei, greatly expanded rod outer segment disks, and larger numbers of inclusion bodies in RPE (p<0.05) were observed in VE (-) group. By fluorescence microscopy, autofluorescence was increased in the RPE layer of VE (-) group. Severe deficiency of VE enhances increased lipid peroxidation and results in structural changes during aging in retina, indicating anti-aging roles of vitamin E.
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[Journal Article] Acceleration of Age-Related Changes in the Retina in a -Tocopherol Transfer Protein Null Mice Fed a Vitamin E-Deficient Diet.2007
Author(s)
Masaki, Tanito, Yasukadu, Yoshida, Sachiko, Kaidzu, Zhi-Hua, Chen, Osamu, Cynshi, Kou-ichi, Jishage, Etsuo, Niki, Akihiro, Ohira
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Journal Title
Investigative Ophthalmology & Vision Science 48 (1)
Pages: 396-404
Description
「研究成果報告書概要(欧文)」より
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