2006 Fiscal Year Final Research Report Summary
Molecular biological study and treatment for diabetic retinopathy
| Project/Area Number |
18591943
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kansai Medical University |
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Principal Investigator |
OGATA Nahoko Kansai Medical University, Faculty of Medicine, Associate Professor (60204062)
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| Co-Investigator(Kenkyū-buntansha) |
WADA Mitsumasa Kansai Medical Univercity, Faculty of Madicine, Assistant Professor (40333215)
JO Nobuo Kansai Medical Univercity, Faculty of Medicine, Instructor (50411533)
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| Project Period (FY) |
2006 – 2007
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| Keywords | diabetic retinopathy / angiogenesis / PEDF / VEGF / cell adhesion molecules / leukostasis |
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| Research Abstract |
Proliferative tissues are found in eyes of some of the SDT (Spontaneously Diabetic Torii) rats, a model of type 2 diabetic mellitus,rats. However, SDT rats do not show non-perfused areas that are different from diabetic retinopathy. Leukocytic activation and the adhesion of leukocytes to the vascular endothelial cells are the causes of capillary occlusion in diabetic retinopathy. Strong interactions between leukocytes and endothelial cells are governed by intracellular adhesion molecule (ICAM)-1. SDT and streptozotocin-induced diabetic (STZ) diabetic rats showed a significant increase of retinal leukostasis compared to that of control rats, but SDT rats had significantly lower levels of leukostasis than STZ rats. The sICAM-1 levels and PEDF expression were up-regulated in both STZ and SDT rats, but the SDT rats showed significantly higher levels of PEDF than STZ rats. In vitro studies showed that exposure of HUVECs to VEGF increased the number of adhering monocytes, and PEDF inhibited the VEGF-induced leukostasis in a dose-dependent manner. The inhibition of the VEGF-induced leukostasis by PEDF is most likely responsible for the low incidence of capillary occlusion and retinal neovascularization in SDT rats.
We investigated the plasma PEDF level and monocytes-derived microparticles (MDMPs) in diabetic patients. MDMPs are increased according to the progression of diabetic retinopathy.
The plasma PEDF level in diabetic patients was significantly higher than that in controls, and the level was especially high in patients with proliferative diabetic retinopathy. The gender, blood urea nitrogen, and triglycerides were significant and independent determinants of plasma PEDF levels in diabetic patients. The PEDF level in the plasma was significantly elevated in diabetic patients, especially those with prolifertive diabetic retinopathy. High levels of PEDF in the plasma may be related to the progression of diabetic retinopathy.
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[Journal Article] Plasma concentration of pigment epithelium-derived factor in patients with diabetic retinopathy.2007
Author(s)
Nahoko Ogata, Masato Matsuoka, Kayako Matsuyama, Chieko Shima, Aran Tajika, Toshiyuki Nishiyama, Mitsumasa Wada, Nobuo Jo, Akiko Higuchi, Keizo Minamino, Hiroshi Matsunaga, Toshihiko Takeda, Miyo Matsumura.
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Journal Title
Journal of Clinical Endocrinology and Metabolism 92
Pages: 1176-1179
Description
「研究成果報告書概要(欧文)」より
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[Presentation] High levels of pigment epithelium-derived factor in blood of patients with diabetic retinopathy.2007
Author(s)
Nahoko Ogata, Masato Matsuoka, Kayao Matsuyama, Chieo Shima, Aran Tajika, Toshiyuki Nishiyama, Mitsumasa Wada, Nobuo Jo, Hiroshi, Matsunaga, Miyo Matsumura.
Organizer
Annual meeting of the Association for Research in Vision and Ophthalmology, Florida
Place of Presentation
USA
Year and Date
20070500
Description
「研究成果報告書概要(欧文)」より
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