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2007 Fiscal Year Final Research Report Summary

The in vitro and in vivo study on effects of growth factor on tooth germs

Research Project

Project/Area Number 18592021
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionAichi Gakuin University

Principal Investigator

KAMEYAMA Yoichiro  Aichi Gakuin University, Emeritus Professor (70113066)

Co-Investigator(Kenkyū-buntansha) MAEDA Hatsuhiko  Aichi-Gakuin University, School of Dentistry, Professor (30175591)
SATO Emiko  Aichi-Gakuin University, School of Dentistry, Lecturer (60111001)
KUBO Katsutoshi  Aichi-Gakuin University, School of Dentistry, Lecturer (60329604)
SUGITA Yoshihiko  Aichi-Gakuin University, School of Dentistry, Lecturer (20367618)
Project Period (FY) 2006 – 2007
Keywordsanimals / iawbone / tooth germ / organ culture / heterocyclic amine / growth factor
Research Abstract

I. In the present study, the effects of both growth factor TGF-α and carcinogen Trp-P-2 on the development of organ-cultured ddY mouse tooth-germ which was transplanted into the subcutaneous tissue of nude mice were investigated histologically, immnohistochemically (PCNA) and histometrically in 5 groups of mice, namely Group A (Control), Group B (DMSO), Group C (TGF-α), Group D (Trp-P-2) and Group E (TGF-α + Trp-P-2). Histologically, in Groups C, D and E, marked proliferation of odontogenic epithelial cells was observed. In Groups D and E, keratinization of the odontogenic epithelial cells was found, but it was not observed in Group C.Moreover, in Group E, a cyst-like space was observed in the area of odontogenic epithelial cells. Immunohistochemically, in Groups C, D and E, PCNA-positivities of the odontogenic epithelial cells were significantly higher than those of Group A.Histometrically, in Groups C and E, the size of the tooth germ was significantly larger in size than that of Gro … More up A. The results of the present study suggest that proliferation, keratinization, cyst-like space formation of odontogenic epitherial cells of transplanted organ-cultured tooth-germs in mice were promoted by growth factor TGF-α and carcinogen Trp-P-2.
II. In the present study, the effects of both carcinogen MeIQx and growth factor TGF-α on the development of organ-cultured ddY mouse tooth-germs were investigated histologically and immnohistochemically (PCNA) in 5 groups of mice, namely Group A (Control), Group B (DMSO), Group C (TGF-α), Group D (MeIQx) and Group E (MeIQx + TGF-α). Histologically, in Groups A and B, both tooth-germs appeared in similar size. In Group C, the tooth-germ was bigger in size than those of Groups A and B. In Groups D and E, the tooth-germs were smaller than in Group C. In Groups C, D and E, marked proliferation of odontogenic epithelial cells was observed. Immunohistochemically, in Groups B, C, F and G, PCNA-positivities of odontogenic epithelial cells were significantly higher than those in Group A. The results of the present study suggested that the proliferation of odontogenic epitherial cells in organ-cultured tooth-germs in mice was promoted by carcinogen MeIQx and growth factor TGF-α.
III. The purpose of the present study was to investigate the effects of growth factor TGF-α and carcinogenen ENU on the development of the transplanted organ-cultured tooth germs in ddY mice. The organ-cultured tooth-germs removed from the 17th day embryo of the ddY mouse were divided into the following 5 groups according their cultured conditions, Group a (Control), Group b (DMSO), Group c (TGF-α), Group d (ENU), and Group e (TGF-α + ENU). These tooth-germs were transplanted into the subcutaneous tissue of the nude mice, and the transplanted organ-cultured tooth-germs were examined histologically and immunohistochemically (PCNA stainings) 2 weeks after transplantation. In Groups c, d and e, proliferation of odontogenic epithelial cells was found in the transplanted organ-cultured tooth-germ. Moreover, in Groups c and e, a cyst-like space was observed in the area of odontogenic epithelial cells. In groups s and e, PCNA-positivities of odontogenic epithelial cells were significantly higherthan that of Group a. The results of the present study indicated that TGF-α and ENU promoted markedly proliferation of odontogenic epithelial cells in the transplanted organ-cultured tooth-germ Less

  • Research Products

    (6 results)

All 2008 2007 2006

All Journal Article (6 results) (of which Peer Reviewed: 3 results)

  • [Journal Article] Transforming Growth Eactor-α とN-ethy1-N-nitrosoureaが器官培養のマウス移植歯胚の発育におよぼす影響2008

    • Author(s)
      小澤 学, 杉田 好彦, 佐藤 恵美子, 久保 勝俊, 神野 正人, 高山 光平, 亀山 洋一郎, 前田 初彦
    • Journal Title

      愛知学院大学歯学会誌 (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Effects of Transforming Growth Factor-α and N-ethyl-N-nitrosourea on the Development of Transplanted Organ-Cultured Mouse Tooth-germ2008

    • Author(s)
      Manabu Ozawa, Yoshihiko Sugita, Emiko Sato, Katsutoshi Kubo, Masato Jinno, Mitsuhiro Takayama, Yoichiro Kameyama, Hatsuhiko Maeda
    • Journal Title

      The Aichi-Gakuin Journal of Dental Science(Japanese) (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] ヘテロサイクリックアミンMeIQxと増殖因子TGF-αがマウスの器官培養歯胚の発育におよぼす影響2007

    • Author(s)
      平井 伸枝, 杉田 好彦, 久保 勝俊, 佐藤 恵美子, 前田 初彦, 亀山 洋一郎
    • Journal Title

      愛知学院大学歯学会誌 45

      Pages: 205-214

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Effects of Heterocyclic Amine MeIQx and Growth Factor TGF-α on the Development of Organ-Cultured Mouse Tooth-germ-Histological and Immunohistochemical Study-2007

    • Author(s)
      Nobue Hirai, Yoshihiko Sugita, Katsutoshi Kubo, Emiko Sato, Hatsuhiko Maeda, Yoichiro Kameyama
    • Journal Title

      The Aichi-Gakuin Journal of Dental Science(Japanese) 45

      Pages: 205-214

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Histological and Immunohistochemical Study on Effects of TGF-α and Trp-P-2 on Transplanted Organ-cultured Mouse Tooth Germ2006

    • Author(s)
      Yoshihiko Sugita, Katsutoshi Kubo, Emiko Sato, Hatsuhiko Maeda and Yoichiro Kameyama
    • Journal Title

      The Journal of Oral Medicine and Pathology 11

      Pages: 75-81

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Histological and Immunohistochemical Study on Effects of TGF-α and Trp-P-2 on Transplanted Organ-cultured Mouse Tooth Germ2006

    • Author(s)
      Yoshihiko Sugita, Katsutoshi Kubo, Emiko Sato, Hatsuhiko Maeda, Yoichiro Kameyama
    • Journal Title

      The Journal of Oral Medicine and Pathology 11

      Pages: 75-81

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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