2007 Fiscal Year Final Research Report Summary
Pathogenic mechanism of severe pneumonia caused by superinfection with influenza virus and oral bacteria
| Project/Area Number |
18592023
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | National Institute of Biomedical Innovation |
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Principal Investigator |
SHIGEFUMI Okamoto National Institute of Biomedical Innovation, National Institute of Biomedical Innovation, Laboratory of Virology and Vaccinology, Researcher Sub-Project Leader (50311759)
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| Co-Investigator(Kenkyū-buntansha) |
KAWABATA Shigetada Osaka University Graduate School of Dentistry, 大学院歯学研究科, Professor (50273694)
OKUNO Yoshinobu Kanonji Institute, The Research Foundation for Microbial Diseases of Osaka University, 観音寺研究所, Director (30112064)
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| Project Period (FY) |
2006 – 2007
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| Keywords | influenza virus / oral streptococci / superinfection / lethal infections / influenza vaccine / adjuvant |
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| Research Abstract |
In the present study, we found that superinfection with non-lethal doses of influenza virus and several kinds of oral streptococci(Streptococcus mutans, S. sanguis, S. salivarius, S. equi) let more than 60% of mice to death. The result suggests that infection with oral streptocci which do not have severe virulent factor can be lethal in addition to prior non-lethal influenza infection. However, it still remains which virulence foctor is a key mediator for induction of lethal influenza-oral streptococcal superinfection.
Immunization of mice with influenza HA vaccine did not protect from the lethal superinfection. We found that influenza HA immunization did not protect from influenza infection with mouse alveolar epithelial cells completely, thus, a few of influenza infected cells were co-infected with oral bacteria. The result indicate that the available vaccine adjuvant that enhances protective immnue responses to delete influenza virus completely is needed for protection from the super
… More
infection. Here we tested whether amphiphilic poly(γ-glutamic acid)-graft-L-phenylalanine copolymers(γ-PGA-NPs), which are derived from a bacterial capsular exopolymer produced by certain Bacillus natto strains, were an effective adjuvant for systemic influenza HA vaccination. Subcutaneous immunization with a mixture of HA vaccine and γ-PGA-NPs induced higher mononuclear cell proliferation and the production of γ-interferon(IFN-γ), interleukin(IL)-4, and IL-6 upon HA restimulation, and enhanced not only anti-HA neutralizing antibody production but also the influenza virus-specific cell-mediated immune response, including CTL activity, compared with immunization with HA alone or a mixture of HA and aluminum adjuvant. HA vaccine with γ-PGA-NPs protected mice against challenges with lethal doses of homologous influenza virus. The results indicate that adding γ-PGA-NPs to the HA vaccine promotes effective protection, and identify γ-PGA-NPs as a new, effective, and potent candidate adjuvant for a subcutaneous influenza virus vaccine. Less
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Research Products
(12 results)
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[Journal Article] Single dose of inactivated Japanese encephalitis vaccine with poly(γ-glutamic acid)nanoparticles provides effective protection from Japanese encephalitis virus.2008
Author(s)
Okamoto, S., Yoshii, H., Ishikawa, T., Akagi, T., Akashi, M., Takahashi, M., Yamanishi, K., Mori, Y.
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Journal Title
Vaccine 26
Pages: 589-594
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Single dose of inactivated Japanese encephalitis vaccine with poly(γ-glutamic acid) nanoparticles provides effective protection from Japanese encephalitis virus.2008
Author(s)
Okamoto, S., Yoshii, H., Ishikawa, T., Akagi, T., Akashi, M., Takahashl, M., Yamanishi, K., Mori, Y.
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Journal Title
Vaccine 26
Pages: 589-594
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Influenza hemagglutinin vaccine with poly(γ-glutamic acid)nanoparticles enhances the protection against influenza virus infection through both humoral and cell-mediated immunity.2007
Author(s)
Okamoto, S., Yoshii, H., Akagi, T., Akashi, M., Ishikawa, T., Okuno, Y., Takahashi, M., Yamanishi, K., Mori, Y.
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Journal Title
Vaccine 25
Pages: 8270-8278
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Influenza hemagglutinin vaccine with poly(γ-glutamic acid) nanoparticles enhances the protection against influenza virus infection through both humoral and cell-mediated immunity.2007
Author(s)
Okamoto, S., Yoshii, H., Akagi, T., Akashi, M., Ishikawa, T., Okuno, Y., Takahashi, M., Yamanishi, K., Mori, Y
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Journal Title
Vaccine 25
Pages: 8270-8278
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Inflammatory immune responses by water-insoluble alpha-glucans from Streptococcus sobrinus.2007
Author(s)
Okamoto, S., Terao, Y., Kaminishi, H., Hamada, S., Kawabata, S.
Organizer
The 2nd International Symposium for Oral Health Science
Place of Presentation
Sendai, Japan
Year and Date
20070218-19
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Enhanced protective immune responses for influenza infection by immunization with influenza HA vaccine and Poly γ glutamic acid nanoparticle.2006
Author(s)
Okamoto, S., Yoshii, H., Ishikawa, T., Akagi, T., Akashi, M., Yamanishi, K., Mori, Y.
Organizer
54th General meeting of the Japanese Society for virology
Place of Presentation
Nagoya, Japan
Year and Date
20061119-21
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Study of effective influenza hemagglutinin vaccine with a novel adjuvant2006
Author(s)
Okamoto, S., Yoshii, H., Ishikawa, T., Akagi, T., Akashi, M., Takahashu M., Yamanishi, K., Mori, Y.
Organizer
10th General meeting of Japanese Society for Vaccinology
Place of Presentation
Izumi-Samo, Osaka, Japan
Year and Date
20061021-22
Description
「研究成果報告書概要(欧文)」より
