2007 Fiscal Year Final Research Report Summary
Elucidation of molecular mechanism underlying smooth muscle-like differentiation of fibroblastic cells derived from periodontal ligament.
| Project/Area Number |
18592026
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
ISHISAKI Akira Hokkaido University, Graduate School of Dentistry, Associate Professor (20356439)
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| Co-Investigator(Kenkyū-buntansha) |
TAMURA Masato Hokkaido University, Graduate School of Dentistry, Professor (30236757)
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| Project Period (FY) |
2006 – 2007
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| Keywords | periodontal ligament / stem cell / differentiation / tube-like structure |
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| Research Abstract |
A blood supply is indispensable for the regeneration of damaged or lost periodontal ligament (PDL) tissue. Mesenchymal stem cell-like activity of cells derived from the PDL has been identified by their capacity to form fibrous and osseous tissue, and cementum. However, it remains to be clarified whether the cells have an ability to build the capillary network of blood vessels. This study evaluated the potential of cells derived from the PDL to construct a blood vessel-like structure, and examined how growth factors controlled the multipotency of the cells.
The ability of a swine PDL fibroblast cell line, TesPDL3, to construct a blood vessel-like structure was evaluated. In addition, the ability of the cells to form mineralized nodules was evaluated on type I collagen-coated plastic plates. In some cases, fibroblast growth factor (FGF)-2 and bone morphogenetic protein (BMP)-2 were added to these cultures. The status of the expression of vascular and osteoblastic cell-specific markers in the cells was evaluated using reverse transcriptase-polymerase chain reaction (RT-PCR), western blotting and immunofluorescent analyses.
TesPDL3 cells not only formed mineralized nodules in response to BMP-2 stimulation but also constructed tube-like structures in response to FGF-2 stimulation. Intriguingly, FGF-2 inhibited the BMP-2-induced mineralized nodule-formation. Conversely, BMP-2 inhibited the FGF-2-induced tube-like structure-formation. Thus, PDL fibroblasts have the potential to differentiate not only into osteoblastic but also into vascular cell lineages. The destiny of the cells was reciprocally regulated by BMP-2 and FGF-2.
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