2007 Fiscal Year Final Research Report Summary
Functions and species differences of softpalate taste buds in mammals
| Project/Area Number |
18592041
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional basic dentistry
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
HARADA Shuitsu Kagoshima University, Graduate School of Medical and Dental Sciences, Professor (60128452)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MIURA Hirohito Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor (80353936)
NAKAYAMA Ayumi Kagoshima University, Graduate School of Medical and Dental Sciences, Assistant Professor (10398290)
TOMONARI Hiroshi Kagoshima University, Graduate School of Medical and Dental Sciences, Assistant Professor (70398288)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Keywords | soft palate / greater superficial petrosal nerve / chorda tvmpani nerve / taste bud / gustatory sensation / development / basal cell / mammal |
|---|
| Research Abstract |
Gustducin was expressed in almost all (96.7%) IP3R3-expressing cells in taste buds of the soft palate (SP),whereas gustducin-positive cells were 42.4% and 60.1 % of IP3R3-expressing cells in the fungiform (FF) and circumvalate (CV) papillae, respectively. To confirm the broad role of gustducin in the taste transduction on the SP, responses from three major gustatory nerves in gustducin-KO mice were recorded electrophysiologically and the response properties were compared among the nerves. In consistent with the immunohistochemical results in the rat, nerve responses to both sweet and bitter stimuli were markedly reduced in the greater superficial petrosal nerve (GSP) of gustducin-KO mice. In contrast to the GSP, the chorda tympani nerve (CT) and the glossopharngeal nerve (GL) showed reduced responses to sweet and bitter stimuli, respectively. Immunohistochemistry of gustducin and IP3R3 in mice showed that 91.1% of IP3R3-expressing cells in the SP was gustducin positive. These results d
… More
emonstrate that gustducin is involved in the different lines of the taste-signaling pathway depending on the taste cell differentiation.
To examine the embryonic development of the basal cells, the expression of the basal cell markers of taste buds (Shh, Proxi and Mashi) was determined in the mouse embryo by in situ hybridization and immunohistochemistry. Proxi was co-expressed with Shh from the beginning of Shh expression in a punctate pattern on the anterior tongue (E1 2.5) and soft palatal region (E14.5),suggesting that the basal cells of taste buds and Shh-expressing spots in embryos share common features. Mashi expression lagged behind the expression of Shh and Proxi by approximately 2 days in both regions. Nerves reached the epithelium expressing Shh slightly before the onset of Mashi expression. These results suggest that the differentiation of the basal cells in the taste bud starts nerve-independently.
We analyzed regenerated taste buds induced by cross-regeneration of CT and GSP in rats and mice using immunohistochemistry and in situ hybridization. The results showed that the gustducin and IP3R3 co-expression between in the SP and FF after the regeneration with the different nerve supply were not depend on the nerve but on the epithelium. This result suggests that the same functional difference still remain after the cross-regeneration. Less
|
-
-
[Journal Article] Expression of the basal cell markers of taste guds in the anterior tongue and soft palate of the mouse embryo2008
Author(s)
Nakayama, A, Miura, H, Shindo, Y, Kusakabe, Y, Tomonari, H, Harada, S
-
Journal Title
J Comp Neurol 509(2)
Pages: 211-224
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
[Journal Article] Expression of gustducin overlaps with that of Type III IP3 receptor in taste buds of the rat soft palate2007
Author(s)
Miura, H, Nakayama, A, Shindo, Y, Kusakabe, Y, Tomonari, H, Harada, S
-
Journal Title
Chem Senses 32(7)
Pages: 689-696
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Distribution pattern and development of basal cells of taste buds during mouse embryogenesis2007
Author(s)
Nakayama, A, Miura, H, Kato, H, Kusakabe, Y, Hino, A, Harada, S
-
Journal Title
Chem Senses 32
Pages: J19
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
[Journal Article] The expression of type III cell marker NCAM and possible cell lineage relationships in mouse taste buds2006
Author(s)
Miura, H, Kato, H, Kusakabe, Y, Nakayama, A, Kariatsumari, M, Ninomiya, Y, Hino, A, Harada, S
-
Journal Title
Chem Senses 31
Pages: J10
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Expressio of the marker genes for the basal cells of taste buds during mouse embryogenesis2006
Author(s)
Nakayama, A, Miura, H, Kato, H, Kusakabe, Y, Hino, A, Harada, S
-
Journal Title
Chem Senses 31
Pages: J10
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Presentation] Cell diiferentitation of the taste buds on the softpalate and fungiform papillae reconnected to different gustatory nerves2008
Author(s)
Harada, S., Tomonari, H., Nakayama, A., Shindo, Y., Kusakabe, Y., Miura, M
Organizer
The 15th International Symposium on Olfaction and Taste
Place of Presentation
San Francisco USA
Year and Date
20080000
Description
「研究成果報告書概要(欧文)」より
-
[Presentation] Eepression of the basal cell markers of taste buds dufing mouse embryonic development2008
Author(s)
Miura, M., Nakayama, A., Shindo, Y., Kusakabe, Y., Tomonari, H., Harada, S
Organizer
The 15th International Symposium on Olfection and Taste
Place of Presentation
San Francisco USA
Year and Date
20080000
Description
「研究成果報告書概要(欧文)」より
-
[Presentation] An exquisitely sensitive Ph biosensor in a marine catfish2008
Author(s)
Caprio, J., Marui, T., Kiyohara, S., Kohbara, J., Harada, S., Mika, Ozaki3, Enoki, K., Shimohara, M
Organizer
The 15th International Symposium on Olfaction and Taste
Place of Presentation
San Francisco USA
Year and Date
20080000
Description
「研究成果報告書概要(欧文)」より
-
[Presentation] The function of the gustducin in the soft palate taste buds differs from that in the tongue2008
Author(s)
Nakayama, A., Tomonari, H., Miura, H., Shindo, Y., Kusakabe, Y., Ninomiya, Y., F Margolskee RF, Harada, S
Organizer
The 15th International Symposium on Olfaction and Taste
Place of Presentation
San Francisco USA
Year and Date
20080000
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
