Analysis of the clock mechanism in salivary gland cells

2008 Fiscal Year Final Research Report

• Project/Area Number: 18592055
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: ONISHI Yoshiaki National Institute of Advanced Industrial Science and Technology, 生物機能工学研究部門, 主任研究員 (60233219)
• Project Period (FY): 2006 – 2008
• Keywords: 転写 / クロマチン / 生物時計

Research Abstract

唾液腺細胞(HSG)において末梢時計機構が存在しており、様々な遺伝子の概日リズム調節の中心をなす時計遺伝子Bmal1遺伝子は転写レベルにおいて概日リズム調節されていることが判明した。このBmal1遺伝子の転写調節は、時計遺伝子特異的なクロマチン構造をしているのみならず、唾液腺細胞(HSG)に特異的な転写調節機構を持っており、これにより様々な唾液腺特異的な生物リズム調節を行っていることが示唆された。

Research Products

• [Journal Article] Molecular characterization of Mybbp1a as a co-repressor on the mPeriod2 promoter (2009)
  • Author(s): Y. Hara, Y. Onishi, K. Ohishi, K. Miyazaki, A. Fukamizu and N. Ishida
  • Journal Title: Nucl. Acids Res. 37 Pages: 1115-1126,
  • Peer Reviewed
• [Journal Article] Rhythmic SAF-A binding underlies circadian transcription of the Bmal1 gene (2008)
  • Author(s): Y. Onishi, S. Hanai, T. Ohno, Y. Hara and N. Ishida
  • Journal Title: Mol. Cell. Biol 28 Pages: 3477-3488
  • Peer Reviewed
• [Journal Article] Identification of negative transcriptional factor E4BP4 binding site in the mouse circadian-regulated gene Mdr2 (2008)
  • Author(s): M. Kotaka, Y. Onishi, T. Ohno and N. Ishida
  • Journal Title: Neurosci. Res 60 Pages: 307-313
  • Peer Reviewed
• [Journal Article] 生物の体内時計-時計遺伝子により構成される洗練された細胞の分子機械 (2008)
  • Author(s): 大西芳秋, 石田直理雄
  • Journal Title: 日本機械学会誌 111 Pages: 756-757
• [Journal Article] The negative transcription factor E4BP4 is associated with circadian clock protein PERIOD2 (2007)
  • Author(s): T. Ohno, Y. Onishi and N. Ishida
  • Journal Title: Biochem. Biophys. Res. Commun 354 Pages: 1010-1015
  • Peer Reviewed
• [Journal Article] A novel E4BP4 element drives circadian expression of mPeriod2 (2007)
  • Author(s): T. Ohno, Y. Onishi and N. Ishida
  • Journal Title: Nucl. Acids Res 35 Pages: 648-655
  • Peer Reviewed
• [Presentation] Rhythmic SAF-A binding underlies circadian transcription of the Bmal1 gene (2008)
  • Author(s): 大西芳秋, 花井修次, 大野.哉, 原康洋, 石田直理雄
  • Organizer: BMB 2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] 時計遺伝子Clockは肝臓でのグリコーゲン合成を制御する (2008)
  • Author(s): 土井亮助, 大石勝隆, 大西芳秋, 石田直理雄
  • Organizer: BMB 2008,
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] 生物時計遺伝子Bmal1のクロマチン構造を介した概日リズム制御 (2008)
  • Author(s): 大西芳秋, 花井修次, 石田直理雄
  • Organizer: JST Innovation Bridge バイオ産業のための革新技術
  • Place of Presentation: 東京
  • Year and Date: 2008-11-26
• [Presentation] Rhythmic SAF-A binding underlies circadian transcription of the Bmal1 gene (2008)
  • Author(s): Y. Onishi, S. Hanai, T. Ohno, Y. Hara and N. Ishida
  • Organizer: 20th Anniversary Meeting SRBR
  • Place of Presentation: Florida
  • Year and Date: 2008-05-18
• [Presentation] Analysis of Chromatin Structure for Clock Gene, Bmal1 Promoter (2007)
  • Author(s): Y. Onishi, T. Ohno, Y. Hara, N. Ishida
  • Organizer: BMB 2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] マウス時計遺伝子Cryptochrome (CRY) 1に結合する新規タンパク質の同定と機能解析 (2007)
  • Author(s): 原康洋, 大西芳秋, 深水昭吉, 石田直理雄
  • Organizer: BMB 2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] Determination of minimal region of rat PERIOD2 interacted with CRYPTOCHROME1 (2007)
  • Author(s): T. Tomita, Y. Onishi, K. Miyazaki, N. Ishida
  • Organizer: International Symposium on Advances in Neurosciences and Silver Jubilee Conference of Indian Academy of Neurosciences
  • Place of Presentation: Varanasi
  • Year and Date: 2007-11-22
• [Presentation] The minimal amino acid sequence of rPER2 necessary to interact with CRY1 (2007)
  • Author(s): T. Tomita, Y. Onishi, K. Miyazaki, N. Ishida
  • Organizer: 2nd World Congress of Chronobiology
  • Place of Presentation: 東京
  • Year and Date: 2007-11-05
• [Presentation] E4BP4 is a negative regulator of circadian clocks (2007)
  • Author(s): Y. Onishi, T. Ohno, N. Ishida
  • Organizer: 7th IBRO World Congress of Neuroscience
  • Place of Presentation: Melbourne
  • Year and Date: 2007-07-15
• [Presentation] Identification and characterization of novel mCRY1-binding proteins (2007)
  • Author(s): Y. Hara, Y. Onishi, N. Ishida
  • Organizer: 7th IBRO World Congress of Neuroscience
  • Place of Presentation: Melbourne
  • Year and Date: 2007-07-13
• [Presentation] mCRY1との相互作用に必要なrPER2 C末端のアミノ酸配列 (2006)
  • Author(s): 冨田辰之介, 大西芳秋, 宮崎歴, 本田真也, 石田直理雄
  • Organizer: 日本分子生物学会2006フォーラム
  • Place of Presentation: 名古屋
  • Year and Date: 2006-12-08
• [Presentation] 生物時計遺伝子産物Cryptochrome (CRY) 1と結合するタンパク質の同定と解析 (2006)
  • Author(s): 原康洋, 大西芳秋, 石田直理雄
  • Organizer: 日本分子生物学会2006フォーラム
  • Place of Presentation: 名古屋
  • Year and Date: 2006-12-07
• [Presentation] Mdr2トランスポーター遺伝子の上流解析 (2006)
  • Author(s): 小高真希, 大西芳秋, 宮崎歴, 大石勝隆, 石田直理雄, 赤池敏宏
  • Organizer: 第13回日本時間生物学会
  • Place of Presentation: 東京
  • Year and Date: 2006-12-02
• [Presentation] 哺乳類体内時計機構におけるE4BP4の役割 (2006)
  • Author(s): 大野朋哉, 大西芳秋, 石田直理雄
  • Organizer: 第13回日本時間生物学会
  • Place of Presentation: 東京
  • Year and Date: 2006-12-02
• [Presentation] Bmal1プロモーターにおけるクロマチン構造を介した転写調節機構 (2006)
  • Author(s): 大西芳秋, 原康洋, 大野朋哉, 石田直理雄
  • Organizer: 第13回日本時間生物学会
  • Place of Presentation: 東京
  • Year and Date: 2006-12-02
• [Presentation] Analysis of Chromatin Structure for Clock Gene, Bmal1 Promoter (2006)
  • Author(s): Y. Onishi, T. Ohno, Y. Hara, N. Ishid
  • Organizer: 20th IUBMB and 11th FAOBMB
  • Place of Presentation: 京都
  • Year and Date: 2006-06-21
• [Presentation] A novel E4BP4 element is required for circadian expression of Period2 (2006)
  • Author(s): T. Ohno, Y. Onishi, N. Ishida
  • Organizer: 20th IUBMB and 11th FAOBMB
  • Place of Presentation: 京都
  • Year and Date: 2006-06-21
• [Book] 時間生物学辞典、クロマチン(著分担) (2008)
  • Author(s): 大西芳秋
  • Total Pages: 218-219(1)
  • Publisher: 朝倉書店
• [Book] 時計遺伝子と環境、きちんとわかる時計遺伝子、産総研ブックス(著分担) (2007)
  • Author(s): 大西芳秋
  • Total Pages: 75-90(15)
  • Publisher: 白日社
• [Patent(Industrial Property Rights)] 生物時計を調節するタンパク質及びその遺伝子 (2008)
  • Inventor(s): 大西芳秋, 花井修次, 原康洋. 石田直理雄
  • Industrial Property Rights Holder: 独立行政法人産業技術総合研究所
  • Industrial Property Number: 特許 特願2008-025966
  • Filing Date: 2008-02-06