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2007 Fiscal Year Final Research Report Summary

The study of the factors of root development and reparative dentine formation

Research Project

Project/Area Number 18592098
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionShowa University

Principal Investigator

MASUDA Yoshiko  Showa University, Dentistry, lecturer (10297038)

Co-Investigator(Kenkyū-buntansha) YANADA Yoshishige  Showa Univ, Dentistry, lecturer (40360127)
Project Period (FY) 2006 – 2007
Keywordsreparative dentine / odontoblast-like cell / proteozlycan
Research Abstract

We prepared the cDNA library as odontoblast-like cell enriched cDNA in vivo with mRNA from the laser irradiated pulp of a rat incisor. The glypican-1 (GPC-1) was detected among the identical genes in the cDNA library as odontoblast-like cell enriched cDNA in vivo. GPC-1 is a member of a family of glycosylphosphatidylinositol anchored cell surface heparin sulfate proteoglycans (HSPG) .
Objectives : The purpose of this study was to identify the expression of HSPG in odontoblast-like cells and evaluate their relation to the formation of reparative dentine.
Methods: 6 and 10 days after Nd: YAG laser irradiation in the pulp, mandibles of rats were dissected and histological procedure was proceeded. We detected the expression of the GPC-1 and Syndecan-1 inununohistologically. To investigate the expression of the GPC-1 gene, Syndecan-1, -2, -3, -4 gene comparing with Tgf-beta 1, osteocalcin, Dspp, we used the cell culture system that reflects the dentine formation in dental pulp tissue. The mandibles were removed from seven male wistar rats (5 weeks old) from the same litter. The pulp tissues were cut into several pieces and incubated in a sterile enzyme solution containing 0.1 % collagenase, 0.05% trypsin. The total RNA were extracted from the cultured cells on days 3, 6, 10 and 15. The RT-PCR amplification was performed.
Results : The immunohistological examination showed stainings of GPC-1 and Syndecan-1 were observed in odontoblast-like cells. In the cell culture system, the expression of mRNA of GPC-1, Syndecan-land Syndecan-4 gradually increased by 15 days, but the expression of Syndecan-2 and Syndecan-3 were almost same during 15 days. The levels of relative quantity mRNA of GPC-1, Syndecan-land syndecan-4 were increased with the process of the odontoblast-like cell differentiation.
Conclusion : These findings suggest that GPC-1, Syndecan-land synclecan-4 might be associated with differentiation of odontoblast-like cell.

  • Research Products

    (10 results)

All 2007 2006

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (4 results)

  • [Journal Article] Analysis of surface roughness of and microleakage of fissure sealants following organic debris removal with Carisolv2007

    • Author(s)
      Yoshishige, Yamada
    • Journal Title

      Journal of Dentistry 36

      Pages: 130-137

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Isolation and partial sequencing of DNA clones from rat incisor after Nd:YAG laser irradiation in root canal2006

    • Author(s)
      Masuda, M.Y., Unno, A., Hossain, M., Okano, T., Matsumoto, K.
    • Journal Title

      Archives of Oral Biology 51

      Pages: 527-534

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Effect of Er,Cr:YSGG laser irradiation on eruption of rat mandibular incisor after disturbance of the enamel organ in the pulp2006

    • Author(s)
      Masuda, M.Y., Wang, X., Hossain, M., Matsuoka, E., Okano T., Matsumoto, K.
    • Journal Title

      Lasers in Medical Science 21

      Pages: 165-169

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Rabbit Ear Chamber法によるMineral Trioxide Aggregateの組織反応の検討2006

    • Author(s)
      増田 宜子, 木村 裕一, 中村 幸生, 松本 光吉
    • Journal Title

      日本歯内療法学会雑誌 27

      Pages: 132-136

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Isolation and partial sequencing of cDNA clones from rat incisor after Nd : YAG laser irradiation in root canal2006

    • Author(s)
      Yoshiko, Masuda
    • Journal Title

      Archives of Oral Biology 51

      Pages: 527-534

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effect of Er, Cr : YSGG laser irradiation on eruption of 'rat mandibular incisor after disturbance of the enamel organ in the pulp2006

    • Author(s)
      Yoshiko, Masuda
    • Journal Title

      Lasers in Medical Science 21

      Pages: 165-69

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Effects of glypican-1 on dentinogenesis in rat dental pulp cells2007

    • Author(s)
      Yoshiko, Masuda
    • Organizer
      127th General meeting of the Conservative Dentistry
    • Year and Date
      20071108-09
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Expression of cell-surface proteoglycan in dental pulp cells2006

    • Author(s)
      Yoshiko, Masuda
    • Organizer
      125th General meeting of the Conservative Dentistry
    • Place of Presentation
      Kagoshima, Japan
    • Year and Date
      20061109-10
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Expression of cell-surface proteoglycans during the reparative dentin process2006

    • Author(s)
      村上 宜子, 王九 虎谷, 山田 嘉重, 松本 光吉
    • Organizer
      IADR(Brisbane)84th General Session & Exhibition of the IADR
    • Place of Presentation
      Brisbane,Australia
    • Year and Date
      20060628-0701
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Expression of cell-surface proteoglycans during the reparative dentin process2006

    • Author(s)
      Yoshiko, Masuda
    • Organizer
      I ADR(Brisbane) 84th General Session& Exhibition of the IADR
    • Place of Presentation
      Brisbane, Australia
    • Year and Date
      20060628-0701
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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