2007 Fiscal Year Final Research Report Summary
A study for mechanism of Epithelial-to-Mesenchymal Transition induced by Autcaine motity Factor
| Project/Area Number |
18592167
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | University of Yamanashi |
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Principal Investigator |
NINAKA Yasufumi University of Yamanashi, Hospital, Assistant Professor (80361715)
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| Project Period (FY) |
2006 – 2007
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| Keywords | squamous cell carcinoma (SCC) / cadherin / vimentin / motility / eoithelial-to-mesenchvmal transition |
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| Research Abstract |
Hereby, we reported that high expression of mesenchymal marker vimentin and low expression of epithelial marker cytokeratins. In highly invasive and metastatic oral squamous carcinoma (SCC) cells. Thus, the invasive cells coexpressed vimentin and cytokeratins, and possessing the mesenchymal features. Introduction of autocrine motility factor (AMF) cDNA into low invasive oral SCC cells showed high level secretion of AMF, allowed for high motile activity and concomittant enhanced vimentin expression and reduced cytokeratin expression, suggesting epithelial-to-mesenchymal transition (EMT). Exogenous AMF also induced high enhancement of vimentin expression and reduction of cytokeratin expression in low invasive SCC cells.
In this study, EMT related gene expression was investigated in seven oral SCC cells and two sarcoma cells. At least two types of EMT could be found in oral SCC cells: down-regulation of E-cadherin and up-regulation of vimentin. The former was closely related with the level of Snail 1 expression, and the latter was correlated with the expression level of Snail3 or ZEB-2/SIP1. Coexpression of Snail 1 and Snail3 was not observed, however, coexpression of Snail 1 and ZEB-2/SIP1 resulted in down-regulation of E-cadherin and up-regulation of vimentin. Thus, the expression of E-cadherin and the expression of vimentin might be regulated by independent mechanism.
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[Journal Article] Fujita, Y., Harada, H., Epithelial-to-Mesenchymal transition in oral squamous carcinoma cells2008
Author(s)
Niinaka, Y., Nakano, Y.,Munakata, M., Higuchi, M., I., wabuchi, D., Matsuda, T
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Journal Title
Japanese Journal of Tissue culture Society For Dental Research 17
Pages: 26-31
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A study on the calcification density of calculus of submandibular gland-Investigation by Peripheral quantitative CT (pQCT)2007
Author(s)
AraiN, Kojima, T., Yamamoto, Y., Motizuki, M., Niinaka, Y., Satoh,Y., Amagasa, T
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Journal Title
Journal of the Japanese Stomatological Society 56
Pages: 26-31
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Epithelial-to-Mesenchymal transition in oral squamous carcinoma cells2008
Author(s)
Niinaka, Y., Nakano, Y., Munakata, M., Higuchi, M., lwabuchi, D., Matsuda, T., Fujita, Y., Harada, H
Organizer
Japanese Journal of Tissue culture Society For Dental Research
Year and Date
2008-12-01
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 口腔扁平上皮癌細胞の上皮間葉移行2007
Author(s)
新中康史, 中野佳央, 宗像源博, 岩渕大珠, 松田健男, 藤田叔宏, 原田清
Organizer
に本口腔組織培養学会
Place of Presentation
愛媛県松山市
Year and Date
2007-12-01
Description
「研究成果報告書概要(和文)」より
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[Presentation] A synovial chondromatosis of the temporomandibular joint with Subcutaneous extension2007
Author(s)
Niinaka, Y., Nakano, Y.,Munakata, M., Higuchi, M., lwabuchi, D., Matsuda, T., Fujita,Y., Harada, H
Organizer
American Association of Oral and maxillo-facial Surgeory
Place of Presentation
Honolulu Hawaii, USA
Year and Date
2007-10-10
Description
「研究成果報告書概要(欧文)」より
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[Presentation] H A synovial chondromatosis of the temporomandibular joint with Subcutaneous extension2007
Author(s)
Niinaka, Y., Nakano, Y.,Munakata, M., Higuchi, M., lwabuchi, D., Matsuda, T., Fujita, Y., Harada
Organizer
Japanese Association of Oral and maxillo-facial Surgery
Place of Presentation
Nagoya Aichi
Year and Date
2007-09-29
Description
「研究成果報告書概要(欧文)」より
