Effect of folic acid on cleft palate prevention induced with dioxins

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 18592179
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Okayama University
• Principal Investigator: YAMADA Tomohiro Okayama University, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Dentistry (60335619)
• Co-Investigator(Kenkyū-buntansha): MISHIMA Katsuaki Okayama University, Hospital, Oral and Maxillofacial Reconstructive Surgery, Senior Assistant Professor (60304317) ; SUGAHARA Toshio Okayama University, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Professor (10116048)
• Project Period (FY): 2006 – 2007
• Keywords: dioxin / mouse / cleft palate / prevention

Research Abstract

In 2006, we examined preventive effect of folic acid for dioxin (TCDD) induced cleft palate in vivo. ICR strain mice of 8-10 weeks of age were used. After 2 weeks administration of folic acid. female mice were impregnated. TCDD was administered with gastric tubes at 40 μg/kg at gestation day (GD) 12, and all fetuses were examined at GD 18.
However, folic acid had no preventive effects on TCDD induced cleft palate.
Then, in 2007, we changed the aim of this research to investigation of the more detailed mechanisms of TCDD induced cleft palate.
In the result of SEM (scanning electron microscope), HE staining, immunohistochemical staining (TGFB3, MSX1, FGFR1/2, LHX8), and organ culture examination, development of fetal palates administered TCDD delayed, but fused as same as normal palates once, however, after fusion phase they ruptured and became cleft palates.

Research Products

• [Journal Article] Morphological and immunohistochemical study on cleft palate induced by 2,3,7,8-Tetrachlorodibenzo-p-dioxin in mice. (2008)
  • Author(s): Fujiwara K, Yamada T, Mishima K, Imura H, Sugahara T
  • Journal Title: Congenital Anomalies 48(2) Pages: 68-73
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Functional requirement of CCN2 for intramembranous bone formation in embryonic mice. (2008)
  • Author(s): Kawaki H, Kubota S, Suzuki A, Yamada T, Matsumura T, Mandai T, Yao M, Maeda T, Lyons KM, Takigawa M.
  • Journal Title: Biochem Biophys Res Commun 366(2) Pages: 450-456
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Respiratory status during sleeping after orthognathic surgery with mandibular setbacks. (2008)
  • Author(s): Yamada T, Mishima K, Matsumura T, Sugii A, Imura H, Sugahara T
  • Journal Title: Asian J Oral Maxillofac Surg 20 Pages: 192-196
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Relationships between nasalance scores and nasopharyngeal shapes in cleft palate patients. (2008)
  • Author(s): Mishima K, Yamada T, Sugii A, Imura H, Sugahara T
  • Journal Title: J Cranio-Maxillofacial Surg 36(1) Pages: 11-14
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Dialectal and gender differences in nasalance scores in a Japanese population. (2008)
  • Author(s): Mishima K, Sugii A, Yamada T, Imura H, Sugahara T
  • Journal Title: J Cranio-Maxillofacial Surg 36(1) Pages: 8-10
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Morphological and immunohistochemical study on cleft palate induced by 2,3,7,8 - Tetrachlorodibenzo - p - dioxin in mice (2008)
  • Author(s): Fujiwara, K., Yamada, T., Mishima, K., Imura, H., Sugahara, T
  • Journal Title: Congenital Anomalies 48(2) Pages: 68-73
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional requirement of CCN2 for intramembranous bone formation in embryonic mice (2008)
  • Author(s): Kawaki, H., Kubota, S., Suzuki, A., Yamada, T., Matsumura, T., Mandai, T., Yao, M., Maeda, T., Lyons, KM., Takigawa, M
  • Journal Title: Biochem Biophys Res Commun 366(2) Pages: 450-456
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Respiratory status during sleeping after orthognathic surgery with mandibular setbacks (2008)
  • Author(s): Yamada, T., Mishima, K., Matsumura, T., Sugii, A., Imura, H., Sugahara, T
  • Journal Title: Asian J Oral Maxiricrac Surg 20(in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Relationships between nasalance scores and nasopharyngeal shapes in cleft palate patients (2008)
  • Author(s): Mishima, K., Yamada, T., Sugii, A., Imura, H., Sugahara, T
  • Journal Title: J Cranio-AkxillofScial Surg 36(1) Pages: 11-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Dialectal and gender differences in nasalance scores in a Japanese population (2008)
  • Author(s): Mishima, K., Sugii, A., Yamada, T., Imura, H., Sugahara, T
  • Journal Title: J Cranio-Maxillofacial Surg 36(1) Pages: 8-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)on the development of the murine palate in organ culture. (2007)
  • Author(s): Yamada T, Fujiwara K, Mishima K, Imura H, Sugahara T
  • Journal Title: Asian J Oral Maxillofac Surg 19(4) Pages: 192-196
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effects of 2,3,7,8- tetrachlorodibenzo- p- dioxin (TCDD) on the development of the murine palate in organ culture (2007)
  • Author(s): Yamada, T., Fujiwara, K., Mishima, K., Imura, H., Sugahara, T
  • Journal Title: Asian J Oral IfkxillofPc Surg 19(4) Pages: 192-196
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on cleft palate-related genes in mouse embryonic palatal mesenchymal cells. (2006)
  • Author(s): Yamada T, Fujiwara K, Mishima K, Imura H, Moritani N, Sugahara T
  • Journal Title: Asian J Oral Maxillofac Surg 18 Pages: 89-94
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Cleft Lip and Palate in Mice Treated with 2,3,7,8-Tetrachlorodibenzo-p-dioxin: a Morphological in vivo Study. (2006)
  • Author(s): Yamada T, Mishima K, Fujiwara K, Imura H, Sugahara T
  • Journal Title: Congenital Anomalies 46(1) Pages: 21-25
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] 岡山大学病院口腔外科(再建系)専門外来における口唇口蓋裂患者の臨床統計調査 (2006)
  • Author(s): 井村英人, 三島克章、由田朋弘、香川智正, 大浦明日香, 菅原利夫
  • Journal Title: 岡山大学歯学雑誌 25(2) Pages: 33-36
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on cleft palate-related genes in mouse embryonic palatal mesenchymal cells (2006)
  • Author(s): Yamada, T., Fujiwara, K., Mishima, K., Imura, H., Moritani, N., Sugahara, T
  • Journal Title: Asian J Oral Maxillofac Surg 18 Pages: 89-94
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cleft Lip and Palate in Mice Treated with 2,3,7,8- Tetrachlorodibenzo-p- dioxin : a Morphological in vivo Study (2006)
  • Author(s): Yamada, T., Mishima, K., Fujiwara, K., Imura, H., Sugahara, T
  • Journal Title: Congenital Anomalies 46(1) Pages: 21-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Clinical study of cleft lip and palate patients at Okayama University Hosoital (2006)
  • Author(s): Imura, H., Mishima, K., Yamada, T., Kagawa, T., Oura, A., Sugahara, T
  • Journal Title: Okayama Univ Dent J 25(2) Pages: 33-36
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Is MFF necessary after a rigid fixation SSRO? (2007)
  • Author(s): Yamada, T., Mishima, K., Matsumura, T., Sugii, A., Imura, H., Sugahara, T
  • Organizer: American Association of Oral and Maxillofacial Surgeons 89th Annual Meeting
  • Place of Presentation: Honolulu
  • Year and Date: 20071000
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] An experimental model of cleft palate exposed to TCDD : an immunohistological examination (2007)
  • Author(s): Imura, H., Yamada, T., Mishima, K., Ueno, T., Matsumura, T., Kondo, S., Hirata, A., Fujiwara, K., Sugii, A., Sugahara, T
  • Organizer: American Association of Oral and Maxillofacial Surgeons 89th Annual Meeting
  • Place of Presentation: Honolulu
  • Year and Date: 20071000
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Is MMF necessary after a rigid fixation SSRO? (2007)
  • Author(s): Yamada, T., Mishima, K., Matsumura, T., Sugii, A., Imura, H., Sugahara, T
  • Organizer: The 52th Japanese Congress of Oral and Maxillofacial Surgery
  • Place of Presentation: Nagoya
  • Year and Date: 20070900
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Production of a range image from a facial motion image and its application for lip motion analysis (2007)
  • Author(s): Mishima, K., Yamada, T., Sugii, A., Imura, H., Sugahara, T
  • Organizer: The 52th Japanese Congress of Oral and Maxillofacial Surgery
  • Place of Presentation: Nagoya
  • Year and Date: 20070900
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] An experimental model of cleft palate exposed to TCDD : an immunohistological examination (2007)
  • Author(s): Imura, H., Yamada, T., Mishima, K., Ueno, T., Matsumura, T., Kondo, S., Hirata, A., Fujiwara, K., Sugii, A., Sugahara, T
  • Organizer: The 52th Japanese Congress of Oral and Maxillofacial Surgery
  • Place of Presentation: Nagoya
  • Year and Date: 20070900
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Mechanism of palatal fusion associated with localization of parlcan and heparanase (2007)
  • Author(s): Hirata, A., Ueno, T., Yamachika, E., Yamada, T., Kagawa, T., Imura, H., Sugahara, T
  • Organizer: The 52th Japanese Congress of Oral and Maxillofacial Surgery
  • Place of Presentation: Naeova
  • Year and Date: 20070900
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Chaos analysis for evaluation of cleft palate speech (2007)
  • Author(s): Ohura, A., Mishima, K., Yamada, T., Imura, H., Sugahara, T
  • Organizer: The 31th Japanese Congress of Cleft Lip and Palate
  • Place of Presentation: Kusatsu
  • Year and Date: 20070500
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Safty of release of MMF in emergency, part 2 (2007)
  • Author(s): Nakano, M., Sugahara, T., Honda, K., Yamada, T., Ueno, T
  • Organizer: The 61th Japanese Congress of Stomatology
  • Place of Presentation: Kobe
  • Year and Date: 20070400
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Is MMF necessary after a rigid fixation SSRO? (2007)
  • Author(s): Yamada T, Mishima K, Matsumura T, Sugii A, Imura H, Sugahara T
  • Organizer: American Association of Oral and Maxillofacial Surgeons 89th Annual Meeting
  • Place of Presentation: Honolulu
  • Year and Date: 2007-10-08
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] An experimental model of cleft palate exposed to TCDD: an immunohist ological examination. (2007)
  • Author(s): Imura H, Yamada T, Mishima K, Ueno T, Matsumura T, Kondo S, Hirata A, Fujiwara K, Sugii A, Sugahara T
  • Organizer: American Association of Oral and Maxillofacial Surgeons 89th Annual Meeting
  • Place of Presentation: Honolulu
  • Year and Date: 2007-10-08
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Production of a range image from a facial motion image and its application for lip motion analysis (2007)
  • Author(s): Mishima K, Yamada T, Sugii A, Imura H, Sugahara T
  • Organizer: American Association of Oral and Maxillofacial Surgeons 89th Annual Meeting
  • Place of Presentation: Honolulu
  • Year and Date: 2007-10-08
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 下顎枝矢状分割術術後における顎間固定の必要性の検討 (2007)
  • Author(s): 山田朋弘、三島克章、松村達志, 杉井明日香、井村英人, 菅原利夫
  • Organizer: 第52回日本口腔外科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-09-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 顔面動画像からの距離画像の作成と口唇運動解析への応用 (2007)
  • Author(s): 三島克章、山田朋弘, 杉井明日香、井村英人, 菅原利夫
  • Organizer: 第52回日本口腔外科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-09-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] TCDDによる口蓋裂誘発機序に関する実験的研究 -免疫組織学的検討- (2007)
  • Author(s): 井村英人, 山田朋弘, 三島克章, 植野高章, 松村達志, 近藤誠二, 平田あずみ, 藤原久美子, 杉井明日香, 菅原利夫
  • Organizer: 第52回日本口腔外科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-09-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] パールカンの動態とヘパラナーゼ局在から検討した口蓋突起癒合のメカニズム (2007)
  • Author(s): 平田あずみ, 植野高章, 山近英樹, 山田朋弘, 香川智正, 井村英人, 菅原利夫
  • Organizer: 第52回日本口腔外科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-09-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 口蓋裂言語評価へのカオス解析の応用 -第一報 リアプノフ数の再現性およびナゾランス値との関連性について. (2007)
  • Author(s): 杉井明日香、三島克章、山田朋弘、井村英人, 菅原利夫
  • Organizer: 第31回口蓋裂学会総会学術集会
  • Place of Presentation: 草津
  • Year and Date: 2007-05-24
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 顎間固定緊急解除時の安全性に関する研究 第2報(固定ワイヤー切断時の口腔内への落下防止について) (2007)
  • Author(s): 中野 誠, 菅原利夫, 本多浩三, 山田朋弘, 植野高章
  • Organizer: 第61回日本口腔科学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2007-04-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Evaluation of respiratory status on sleeping after orthognathic surgery with mandibular setback (2006)
  • Author(s): Yamada, T., Mishima, K., Ohura, A., Imura, H., Sugahara, T
  • Organizer: The 51th Japanese Congress of Oral and Maxillofacial Surgery
  • Place of Presentation: Kitakyusyu
  • Year and Date: 20061000
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Safty of release of MKT in emergency (2006)
  • Author(s): Nakano, M., Sugahara, T., Honda, K., Ueno, T., Yamada, T., Sawaki, K., Tei, Y., Aki, S
  • Organizer: The 51th Japanese Congress of Oral and Maxillofacial Surgery
  • Place of Presentation: Kitakyusyu
  • Year and Date: 20061000
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Nasolance score in normal population (2006)
  • Author(s): Ohura, A., Mishima, K., Yamada, T., Imura, H., Sugahara, T
  • Organizer: The 51th Japanese Congress of Oral and Maxillofacial Surgery
  • Place of Presentation: Kitakyusyu
  • Year and Date: 20061000
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Evaluation of respiratory status on sleeping after orthognathic surgery with mandibular setback (2006)
  • Author(s): Yamada T, Mishima K, Ohura A, Imura H, Matsumura T, Sugahara T
  • Organizer: The 7th Asian Congress of Oral and Maxillofacial Surgery
  • Place of Presentation: Hong Kong
  • Year and Date: 2006-11-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 下顎骨後方移動術前後の睡眠時呼吸状態の評価. (2006)
  • Author(s): 山田朋弘, 三島克章, 大浦明日香, 井村英人, 菅原利夫
  • Organizer: 第51回日本口腔外科学会総会
  • Place of Presentation: 北九州
  • Year and Date: 2006-10-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 顎間固定装置緊急解除時の安全性に関する研究 (2006)
  • Author(s): 中野 誠, 菅原利夫, 本多浩三, 植野高章, 山田朋弘, 澤木康一, 鄭 吉弘, 佐藤亜希
  • Organizer: 第51回日本口腔外科学会総会
  • Place of Presentation: 北九州
  • Year and Date: 2006-10-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 健常者におけるナゾランス値の比較検討. (2006)
  • Author(s): 大浦明日香, 三島克章, 山田朋弘, 井村英人, 菅原利夫
  • Organizer: 第51回日本口腔外科学会総会
  • Place of Presentation: 北九州
  • Year and Date: 2006-10-12
  • Description: 「研究成果報告書概要(和文)」より