Visualization of Localization of 14C-labeled Lidocaine in Oral Tissue

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 18592204
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: MAMIYA Hideki Tokyo Dental College, Dental Anesthesiology, Assistant Professor (30266572)
• Co-Investigator(Kenkyū-buntansha): ICHINOHE Tatsuya Tokyo Dental College, Dental Anesthesiolog, Professor (40184626) ; KANEKO Yuzuru Tokyo Dental College, Dental Anesthesiology, Professor (00085747) ; ANZAI Kazunori National Institute of Radiological Sciences, 放射線安全研究センター, Team Leader (70128643)
• Project Period (FY): 2006 – 2007
• Keywords: Autoradiography / Radioisotope / Local Anesthetics / Drug Distribution

Research Abstract

Localization of lidocaine in oral mucosa of the rabbit was visualized using imaging plate autoradiography. Under general anesthesia, 14C-labeled 2% lidocaine solution with or without epinephrine was injected into oral mucosa or periodontal ligament of rabbits. Five minutes after injection, animals were killed and frozen. Slices of animals' head were obtained using a cryomicrotome. Bio-Imaging Analyzer System was used to visualize the accumulation of 14C in samples. To determine localization of 14C-labeled lidocaine in oral tissue, we scanned the same sliced sample and superimposed the two obtained images. Injected 14C-labeled lidocaine was clearly visualized. Localization of lidocaine with epinephrine around the root apex was more apparent than that without epinephrine. Labeled lidocaine injected into the periodontal ligament was observed along the root surface. This method thus appears to be useful for visualizing the localization of lidocaine in tissue.

Research Products

• [Presentation] 局所麻酔薬投与方法による組織内浸透経路の差異-14Cを用いた歯根膜内麻酔法と骨膜上麻酔法の比較 (2007)
  • Author(s): 間宮 秀樹, 山崎 貴希, 中村 公英, 安西 和紀, 一戸 達也, 金子 譲
  • Organizer: 第35回 日本歯科麻酔学会総会
  • Place of Presentation: 北九州国際会議場
  • Year and Date: 2007-10-04
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 投与後の経過時間が兎の口腔組織内14C標識リドカインの局在性に与える影響 (2007)
  • Author(s): 山崎 貴希, 間宮 秀樹, 中村 公英, 安西 和紀, 一戸 達也, 金子 譲
  • Organizer: 第35回 日本歯科麻酔学会総会
  • Place of Presentation: 北九州国際会議場
  • Year and Date: 2007-10-04
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Comparison of drug distribution of local anesthetics administered by parastermal or periodontal ligament injection using 14C (0)
  • Author(s): Hideki, Mamiya, Takaki, Yamazaki, Kimihide, Nakamura, Kazunori, Anzai, Tatsuya, Ichinohe, Yuzuru, Kaneko
  • Organizer: 35th Japanese Dental Society of Anesthesiology
  • Place of Presentation: Kita-Kyusyu city
  • Year and Date: 00001004
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The effect of the time course to drug distribution of local anesthetics administered in oral tissue of rabbit using 14C (0)
  • Author(s): Takaki, Yamazaki, Hideki, Mamiya, Kimihide, Nakamura, Tatsuya, Ichinohe, Kazunori, Anzai, Yuzuru, Kaneko
  • Organizer: 35th Japanese Dental Society of Anesthesiology
  • Place of Presentation: Kita-Kyusyu city
  • Year and Date: 00001004
  • Description: 「研究成果報告書概要(欧文)」より