2007 Fiscal Year Final Research Report Summary
Molecular analysis of gloom-binding Protein in canes Pathogenesis of S. mutans
| Project/Area Number |
18592233
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
NAKANO Michiyo Osaka University, Graduate Schcol of Dentistry, Assistant Professor (30359848)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Dental caries / Streptococcus mutans / glucan-binding protein / Molecular microbiology |
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| Research Abstract |
Streptococcus mutans has been implicated as a major causative agent of dental caries in humans. Bactreial components associated with the adhesion phase of S. mutans include glucosyltransferase, protein antigen c, and proteins that bind glucan. At least four glucan-binding proteins (Gbp) have been identified; GbpA, GbpB, GbpC, and GbpD. In our previous study,the contributions of GbpA and GbpC to the virulence of S. mutans were investigated; however, the biological function of GbpB and its role in the virulence of S. mutans remain to be elucidated. Using a GbpB-deficient mutant strain (BD1), we demonstrated in the present study that GbpB has a role in the biology of S. mutans. The growth rate of BD1 was lower than that of other strains, while it was also shown to be less susceptible to phagocytosis and to form longer chains than the parental strain MT8148. In addition, electron microscope observations of the cell surfaces of BD1 showed that call-wall layers were obscure. These results suggest that GbpB may have an important role in the cell-wall construction and be involved in cell separation and cell maintenance.
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