2007 Fiscal Year Final Research Report Summary
Prevention of alveolar bone resorption and athrosclerosis through the regulation of OPG gene expression
| Project/Area Number |
18592258
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NAGASAWA Toshiyuki Tokyo Medical and Dental University, Graduate school, Department of hard tissue engineering, Research associate (90262203)
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| Co-Investigator(Kenkyū-buntansha) |
NITTA Hiroshi Graduate school, Tokyo Medical and Dental University, Department of Comprehensive Oral Care, Associate Professor (70237767)
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| Project Period (FY) |
2006 – 2007
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| Keywords | OPG / RANKL / atherosclerosis / P. gingivalis |
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| Research Abstract |
Chronic inflammation and infection play pivotal roles in the pathogenesis of abdominal aortic aneurysm (AAA). Receptor activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG) have recently been implicated as key partners of osteoimmunology and vascular diseases. Although periodontopathic bacteria were detected in AAA specimens, the role of periodontal infection in AAA pathogenesis remains unclear. This study investigated the association of periodontopathic bacteria with the expression of RANKL and OPG in AAA.
AAA and normal aorta tissues were collected from patients during surgical AAA repair. Expression of RANKL and OPG mRNA was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and localization of RANKL in AAA tissues was examined by immunohistochemistry. Periodontopathic bacteria in specimens were detected by PCR. Our results showed significantly increased RANKL, but decreased OPG, mRNA levels in AAA tissues when compared to normal aorta tissues. RANKL was localized in the intimal and medial layers and was expressed in mononuclear and vascular smooth muscle cells (VSMCs). The expression levels of RANKL, but not OPG, were positively correlated with the presence of Porphyromonas gingivalis and Treponema denticola, but not A. actinomycetemcomitans, in vessel tissues. These periodontopathic bacteria were not detected in any normal tissues. P. gingivalis lipopolysaccharide was able to upregulate RANKL expression in VSMCs. Our findings demonstrated the association between periodontopathic bacteria and RANKL upregulation in AAA, suggesting a potential role of periodontal infection in the pathogenesis of AAA, via RANKL upregulation.
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Research Products
(13 results)
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[Journal Article] Functional polymorphisms of the FPR1 gene and aggressive periodontitis in Japanese.2007
Author(s)
Gunji T, Onouchi Y, Nagasawa T, Katagiri S, Watanabe H, Kobayashi H, Arakawa S, Noguchi K, Hata A, Izumi Y, Ishikawa I.
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Journal Title
Biochem Biophys Res Commun 364 (1)
Pages: 7-13
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Functional polymorphisms of the FPR1 gene and aggressive periodontitis in Japanese. Biochem Biophys Res Commun.2007
Author(s)
Gunji T, Onouchi Y, Nagasawa T, Katagiri S, Watanabe H, Kobayashi H, Arakawa S, Noguchi K, Hata A, Izumi Y, Ishikawa I.
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Journal Title
Biochem Biophys Res Commun. 364(1)
Pages: 7-13
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Molecular mimicry of Actinobacillus actinomycet emcomitans with beta-2 glycoprotein I2007
Author(s)
Nagasawa T, Wang D, Chen YW, Ushida Y, Kobayashi H, Takeuchi Y, Umeda M, Izumi Y
Organizer
American Academy of Periodontology 93rd Annual meeting
Place of Presentation
The Walter E. Washington Convention Center, Washington, DC, USA)
Year and Date
2007-10-29
Description
「研究成果報告書概要(欧文)」より
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