2007 Fiscal Year Final Research Report Summary
Relationship between lipopolysaccharide and reactive oxygen species in rat periodontitis model
| Project/Area Number |
18592277
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
YAMAMOTO Tetsuo Okayama University, Hospital, Lecturer (20252984)
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| Co-Investigator(Kenkyū-buntansha) |
TOMOFUJI Takaaki Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Assistant Professor (80335629)
EKUNI Daisuke Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Assistant Professor (70346443)
YAMANAKA Reiko Okayama University, Hospital, Assistant Professor (00379760)
WATANABE Tetsuo Okayama University, Professor Emeritus (20034176)
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| Project Period (FY) |
2006 – 2007
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| Keywords | periodontitis / rat / lipopolvsaccharide / reactive oxygen species / fatty liver / oxidative stress / cytokines / high cholesterol diet |
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| Research Abstract |
Our previous study demonstrated that topical application of bacterial pathogens, i.e., lipopolysaccharide (LPS) and bacterial proteases, to gingival sulcus induced fatty liver. The purpose of this research project was to clarify the mechanism of fatty liver in rat periodontitis model, especially, to investigate the relationship between LPS and reactive oxygen species (ROS).
In the year 2006, serum levels of LPS, ROS and tumor necrosis factor (TNF)-alpha were found to be elevated in rats with periodontitis compared to rats without periodontitis. Moreover, both concentrations of 8-hydroxydeoxyguanosine (8-OHdG) in liver homogenate and densities of 8-OHdG-positive liver cells were larger in the rats with periodontitis than in those without These results suggest that topically applied ITS affects liver directly, that cells in the periodontium respond LPS and produce ROS, that the ROS produced in periodontium affects liver directly, and that cytokines play some roles in the pathogenesis of fatty liver induced by periodontitis.
In the year 2007, the effects of high cholesterol diet on the markers of oxidative stress in blood and liver of rats with periodontitis were investigated. Serum levels of reactive oxygen metabolites and hexanoyl-lysine and liver level of 8-OHdG were increased after feeding high cholesterol diet in the rats with periodontitis. These results suggest that high cholesterol diet has additive effects on periodontitis-induced oxidative damage in blood and liver.
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[Journal Article] Mechanical stimulation of gingiva reduces plasma 8-OHdG level in rat periodontitis2008
Author(s)
Ekuni, D., Tomofuji, T., Tamaki, N., Sanbe, T., Azuma, T., Yamanaka, R., Yamamoto, T., Watanabe, T
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Journal Title
Archives of Oral Biology 53(in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Chronic administration of lipopolysaccharide and proteases induces periodontal inflammation and hepatic steatosis in rats2007
Author(s)
Tomofuji, T., Ekuni, D., Yamanaka, R., Kusano, H., Azuma, T., Sanbe, T., Tamaki, N., Yamamoto, T., Watanabe, T., Miyauchi, M., Takata, T
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Journal Title
Journal of Periodontology 78
Pages: 1999-2006
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Oral administration of vitamin C prevents alveolar bone resorption induced by high dietary cholesterol in rats2007
Author(s)
Sanbe, T., Tomofuji, T., Ekuni, D., Azuma, T., Tamaki, N., Yamamoto, T
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Journal Title
Journal of Periodontology 78
Pages: 2165-2170
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effects of toothbrushing on periodontitis-induced liver diseases2007
Author(s)
Sanbe, T., Tomofuji, T., Ekuni, D., Yamanaka, R., Azuma, T., Tamaki, N., Yamamoto, T., Watanabe, T
Organizer
56th Meeting of the Japanese Society for Dental Health
Place of Presentation
Tokyo
Year and Date
20071000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Oxidative damage of various organs in a rat periodontitis model2007
Author(s)
Tamaki, N., Tomofuji, T., Ekuni, D., Azuma, T., Sanbe, T., Yamamoto, T., Watanabe, T
Organizer
International Academy of Periodontology Ilth International Biennial Congress
Place of Presentation
Berne, Switzerland
Year and Date
20070900
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effects of toothbrushing on serum 8-OHdG level in periodontitis model rats2007
Author(s)
Tamaki, N., Tomofuji, T., Ekuni, D., Azuma, T., Yamanaka, R., Sanbe, T., Yamamoto, T., Watanabe, T
Organizer
18th Meeting of the Japanese Society for Dental Health, Kinki-Chugoku-Shikoku Branch
Place of Presentation
Osaka
Year and Date
20070600
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effect of toothbrushing on liver disease in the rat periodontitis model2007
Author(s)
Sanbe, T., Tomofuji, T., Ekuni, D., Yamanaka, R., Azuma, T., Tanamki, N., Yamamoto, T., Watanabe, T., Miyauchi, M., Takata, T
Organizer
50th Annual Meeting of the Japanese Society of Periodontology in Spring
Place of Presentation
Yokosuka
Year and Date
20070400
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effects of a high-cholesterol diet on bone mineral density of the alveolar bone in rats2007
Author(s)
Tomofuji, T., Ekuni, D., Azuma, T., Sanbe, T., Yamamoto, T., Watanabe, T
Organizer
50th Annual Meeting of the Japanese Society of Periodontology in Spring
Place of Presentation
Yokosuka
Year and Date
20070400
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Elevated serum lipopolysaccharide induces liver injury in the rat periodontitis model2006
Author(s)
Tomofuji, T., Ekuni, D., Yamanka, R., Azuma, T., Sanbe, T., Yamamoto, T., Watanabe, T., Miyauchi, M., Takata, T
Organizer
7th Congress of the Asian Academy of Preventive Dentistry
Place of Presentation
Okayama
Year and Date
20061100
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effects of mechanical stimulation on experimentally-induced periodontitis in a rat model2006
Author(s)
Yamamoto, T., Tomofuji, T., Ekuni, D., Yamanaka, R., Azuma, T., Kusano, H., Sanbe, T., Watanabe, T
Organizer
49th Annual Meeting of the Japanese Society of Periodontology in Autumn
Place of Presentation
Osaka
Year and Date
20061000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Nonalcoholic steatohepatitis in rat periotontitis model2006
Author(s)
Ekuni, D., Tomofuji, T., Yamanaka, R., Kusano, H., Azuma, T., Sanbe, T., Tamaki, N., Yamamoto, T., Watanabe, T., Miyauchi, M., Takata, T
Organizer
49th Annual Meeting of the Japanese Society of Periodontology in Autumn
Place of Presentation
Osaka
Year and Date
20061000
Description
「研究成果報告書概要(欧文)」より
