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2008 Fiscal Year Final Research Report

Exploring heterologous overexpression system and modular assembly of archaeal metalloenzyme complexes

Research Project

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Project/Area Number 18608004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 極限環境生物学
Research InstitutionNippon Medical School

Principal Investigator

IWASAKI Toshio  Nippon Medical School, 医学部, 講師 (40277497)

Co-Investigator(Kenkyū-buntansha) KUMASAKA Takashi  (財)高輝度光科学研究センター, 利用研究促進部門, 副主席研究員 (30291066)
OHMORI Daijiro  順天堂大学, 医学部, 教授 (00124967)
Project Period (FY) 2006 – 2008
Keywords酵素 / 蛋白質 / 極限環境生物 / 古細菌 / 微生物 / 機能生化学 / 鉄硫黄クラスター
Research Abstract

超好熱菌エネルギー代謝系の主要複合金属酵素は、学術上重要なだけでなく、バイオナノセンサーやエレクトロナノテクノロジー等への産業利用も見込まれる。ポストゲノム・ポストストラクチャー時代の応用展開を考え、超好熱菌の複合金属酵素をコードする遺伝子クラスターの簡易発現系ストラテジーの大枠を考案した。各種分光学的解析、結晶構造解析等と合わせて検討・改善を重ね、当初の主たる研究目標をほぼ達成できた。

  • Research Products

    (9 results)

All 2008 2007 2006

All Journal Article (6 results) (of which Peer Reviewed: 5 results) Presentation (3 results)

  • [Journal Article] Crystallization and preliminary X-ray diffraction studies of the prototypal homologue of mitoNEET (Tth-NEET0026) from the extreme thermophile Thermus thermophilus HB82008

    • Author(s)
      Asako Kounosu, Toshio Iwasaki, Seiki Baba, Yoko Hayashi-Iwasaki, Tairo Oshima, Takashi Kumasaka
    • Journal Title

      Acta Crystallogr. Sect. F 64

      Pages: 1146-1148

    • Peer Reviewed
  • [Journal Article] Shimizu, Takashi Kumasaka (2007) Crystallization and preliminary X-ray diffraction studies of the ISC-like [2Fe-2S] ferredoxin fPseudomonas putida JCM 200042007

    • Author(s)
      Toshio Iwasaki, Daijiro Ohmori, Nobutaka Shimizu, Takashi Kumasaka
    • Journal Title

      Crystallogr. Sect. F 63

      Pages: 1014-1016

    • Peer Reviewed
  • [Journal Article] Modular structure and assembly of archaeal respiratory complex II2007

    • Author(s)
      Toshio Iwasaki, Daijiro Ohmori, Asako Kounosu
    • Journal Title

      ProceedingInternational Symposium on Extremophiles and Their Applications 2005

      Pages: 329-329

  • [Journal Article] Crystallization and preliminary X-ray diffraction studies of the hyperthermophilic Rieske protein variant (SDX-triple) with an engineered rubredoxin-like mononuclear iron site2006

    • Author(s)
      Toshio Iwasaki, Asako Kounosu, Daijiro Ohmori, Takashi Kumasaka
    • Journal Title

      Acta Crystallogr. Sect. F 62

      Pages: 993-995

    • Peer Reviewed
  • [Journal Article] Resonance Raman characterization of archaeal and bacterial Rieske protein variants with modified hydrogen bond network around the [2Fe-2S] center.2006

    • Author(s)
      Toshio Iwasaki, Asako Kounosu, Derrick R.J. Kolling, Sangmoon Lhee, Antony R. Crofts, Sergei A. Dikanov, Takuro Uchiyama, Takashi Kumasaka, Hiroyuki Ishikawa, Miwa Kono, Takeo Imai, Akio Urushiyama
    • Journal Title

      Protein Sci. 15

      Pages: 2019-2024

    • Peer Reviewed
  • [Journal Article] Dikanov. (2006) ^<15>N HYSCORE characterization of the fully deprotonated, reduced form of the archaeal Rieske [2Fe-2S] center2006

    • Author(s)
      Toshio Iwasaki, Asako Kounosu, Rimma I. Samoilova, Sergei A.
    • Journal Title

      j.Am.Chem.Soc. 128

      Pages: 2170-2171

    • Peer Reviewed
  • [Presentation] 大腸菌を宿主とする超好熱性古細菌の遺伝子クラスターの簡易発現系2008

    • Author(s)
      鴻巣麻子, 他
    • Organizer
      第3日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
  • [Presentation] インシュリン抵抗性改善薬標的タンパク質mitoNEETと好熱菌ホモログの新規鉄硫黄クラスターの解析2008

    • Author(s)
      大森大二郎, 他
    • Organizer
      第3物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
  • [Presentation] モデル系としての[2Fe-2S]タンパク質の結晶構造と ^<15>N HYSCORE 解析2007

    • Author(s)
      鴻巣麻子, 他
    • Organizer
      第80回日本生化学会大学合同大学
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-14

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Published: 2010-06-10   Modified: 2016-04-21  

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