2007 Fiscal Year Final Research Report Summary
Analysis of pain related gene expression in endometriosis and endometriosis related pelvic pain
| Project/Area Number |
18613017
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pain science
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| Research Institution | Keio University |
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Principal Investigator |
ASADA Hironori Keio University, School of Medicine, Assistant Professor (60231883)
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| Co-Investigator(Kenkyū-buntansha) |
KAJITANI Takashi Keio University, School of Medicine, Instructor (60407111)
MARUYAMA Tetsuo Keio University, School of Medicine, Assistant Professor (10209702)
YOSHIMURA Yasunori Keio University, School of Medicine, Professor (10129736)
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| Project Period (FY) |
2006 – 2007
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| Keywords | endometriosis / NGF / TrkA / p75NTR |
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| Research Abstract |
Endometriosis is a poorly understood, estradiol-dependent condition associated with severe pelvic pains and defined by vascularized endometrial growths outside the uterus. However, the mechanisms by which pain is generated remain unknown. The broad scope of neurotrophin action is well documented in the case of nerve growth factor (NGF) and its effect on nociceptors and nociception. It was reported that NGF is up-regulated after inflammatory injury and administration of exogenous NGF either systemically or in the skin causes thermal hyperalgesia. We examined NGF, TrkA receptor and p75 receptor expression in the endometriotic lesion and normal endometrium by means of immunohistochemistry, RT-PCR and Western blot. Endometrial cyst wall, adenomyotic lesions, peritoneal endometriotic lesions and normal endometrium were prepared (n=19, 4, 8 and 8, respectively). NGF expression was significantly stronger in peritoneal endometriosis, endometriotic cyst, and adenomotic lesions than in normal endometrium. NGF expression was the most stronger in peritoneal lesions. Although the low affinity neurotrophin receptor (p75NTR) was detected in the endometriotic lesions, high affinity NGF receptor (TrkA) was not detectied in the endometriotic lesions. The levels of NGF in peritoneal fluid were also measured to compare the concentration in women who have endometriosis and in women without endometriosis. NGF was detected in 45% cases. 5 of the 18 control samples (28%) was positive. Of the 46 samples of endometriosis patients, 24 was positive for NGF (52%). These results suggest a role of NGF in the progression of endometriosis. The pain and hyperalgesia in endometriosis may be related with the expression of NGF. The expression of the NGF- p75NTR could explain hyperalgesia in endometriosis.
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[Journal Article] Histone deacetylase inhibitors stimulate cell migration in human endometrial adenocarcinoma cells through up-regulation of glycodelin2007
Author(s)
Uchida, H, Maruyama, T, Ono, M, Ohta, K, Kajitani, T, Masuda, H, TNagashima, T, Arase, T, Asada, H, Yoshimura, Y
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Journal Title
Endocrinology 148
Pages: 896-902
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Side population in human uterine myometrium displays phynotypic and functional characteristics of myometrial stem cells2007
Author(s)
Ono, M, Maruyama, T, Masuda, H, Kajitani, T, Nagashima, T, Arase, T, Ito, M, Ohta, K, Uchida, H, Asada, H, Yoshimura, Y, Okano, H, Matsuzaki, Y
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Journal Title
Proc Natl Acad Sci USA 104
Pages: 18700-18705
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Expression and localization of nerve growoth factor in the endometriotic and adenomyotic lesions2007
Author(s)
Kajitani, T, Maruyama, T, Asada, H, Uchida, H, Masuda, H, Nagashima, T, Ono, M, Arase, T, Ohta, K, Kagami, M, Yoshimura, Y
Organizer
54th Annual Scientific Meeting of Society for Gynecologic Investigation
Place of Presentation
Reno(USA)
Year and Date
2007-03-15
Description
「研究成果報告書概要(欧文)」より
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[Book] 子宮筋腫,別冊日本臨床2006
Author(s)
丸山 哲夫、浅田 弘法, ほか
Total Pages
477-484
Publisher
日本臨床社
Description
「研究成果報告書概要(和文)」より
