2008 Fiscal Year Final Research Report
Plasmid-mediated augmentation for wastewater treatment
| Project/Area Number |
18681013
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Osaka University |
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Principal Investigator |
SOUDA Satoshi Osaka University, 大学院・工学研究科, 准教授 (30322176)
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| Project Period (FY) |
2006 – 2008
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| Keywords | 難分解性物質 / 廃水処理 / プラスミド |
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| Research Abstract |
2,4-ジクロロフェノキシ酢酸(2,4-Dを部分分解できるプラスミドpJP4を用い、連続回分式活性汚泥プロセス(SBR)にプラスミドオーグメンテーションを実施した。pJP4を導入しなかった対照系SBRは、2,4-Dを分解できなかった。2,4-Dを完全分解できるJMP134 (pJP4)を導入したSBRは、3日目まで高い分解率を示した後、JMP134の淘汰に伴って分解率が低下し、transconjugantが増加した20日目から分解率が回復した。2,4-Dを分解できないHB101 (pJP4)を導入したSBRは、7日間は2,4-Dを分解できなかったが、transconjugantが増加した20日目から分解率が向上した。
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[Journal Article] Occurrence and persistence of indigenous transconjugants carrying conjugative plasmids in soil.2009
Author(s)
Inoue, D., Soda, S., Tsutsui, H., Yamazaki, Y., Murashige, K., Sei, K., Fujita, M., and Ike, M.
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Journal Title
J. Biosci. Bioeng. in press
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[Journal Article] Transfer of antibiotic multiresistant plasmid RP4 from Escherichia coli to activated sludge bacteria.2008
Author(s)
Soda, S., Ohtsuki, H., Inoue, D., Tsutsui, H., Sei, K., and Ike, M.
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Journal Title
J. Biosci. Bioeng. 106(3)
Pages: 2929-296
Peer Reviewed
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[Presentation] Transfer of plasmid pJP4 from Escherichia coli and Pseudomonas putida to activated sludge-derived bacteria by filter mating.2009
Author(s)
Tsutsui, H., Matsuda, M., Anami, Y., Inoue, D., Sei, K., Soda, S., and Ike, M.
Organizer
International Symposium on Frontiers of Environmental and Industrial Biotechnology
Place of Presentation
豊中
Year and Date
2009-01-21
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[Presentation] Occurrence and persistence of indigenous transconjugants carrying conjugative plasmids in soil microcosms.2008
Author(s)
Inoue, D., Soda, S., Tsutsui, H., Yamazaki,Y., Murashige, K., Sei, K., Fujita, M., and Ike, M.
Organizer
International Biotechnology Symposium and Exhibition
Place of Presentation
中国大連
Year and Date
2008-10-14
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[Presentation] Transfer of plasmid pJP4 from Escherichia coli by filter mating to and its expression in activated sludge-derived bacteria2008
Author(s)
Tsutsui, H., Takeda, S., Otsuki, H., Inoue, D., Sei, K., Soda, S., and Ike, M.
Organizer
International Biotechnology Symposium and Exhibition
Place of Presentation
中国大連
Year and Date
2008-10-13
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