Molecular Mechanism of Photoprotective Function of a Carotenoid Bound to the Photoreaction Center Revealed by Time-Resolved X-Ray Crystallography

2008 Fiscal Year Final Research Report

• Project/Area Number: 18684016
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Condensed matter physics I
• Research Institution: Osaka City University
• Principal Investigator: FUJII Ritsuko Osaka City University, 大学院・理学研究科, 博士研究員 (80351740)
• Project Period (FY): 2006 – 2008
• Keywords: 光物性 / 光合成 / たんぱく質構造 / 物性 / 光誘起協力現象 / エネルギー散逸

Research Abstract

紅色光合成細菌Rhodobacter sphaeroides 2.4.1由来の光反応中心複合体の1.95Åの高分解能X線結晶構造解析に日本で初めて成功した。その結果、光合成光保護メカニズムに関与すると考えられるカロテノイドの結合長の変位を検出するためには2.5Å以上の高分解能が必須であることがわかった。また分解能を左右するクライオプロテクションなどの操作を改良することにより、従来よりも小さい光を透過する結晶でも高分解能を得ることに成功した。

Research Products

• [Journal Article] Construction of Hybrid Photosynthetic Units Using Peripheral and Core Antennae from Two Different Species of Photosynthetic Bacteria : Detection of the Energy Transfer from Bacteriochlorophyll a in LH2 to Bacteriochlorophyll b in LH1 (2008)
  • Author(s): R. Fujii, S. Shimonaka, N. Uchida, A. T. Gardiner, R. J. Cogdell, M. Sugisaki, and H. Hashimoto,
  • Journal Title: Photosyn. Res 95 Pages: 327-337
  • Peer Reviewed
• [Journal Article] Femtosecond Time-Resolved Absorption Spectra of Photosynthetic Membranes from a Purple Bacterium Blastochloris (Rhodopseudomonas) viridis (2007)
  • Author(s): R. Fujii, N. Wakatake, S. Shimonaka, Q.-D. Chen, M. Sugisaki, A. T. Gardiner, R. J. Cogdell, H. Hashimoto
  • Journal Title: Carotenoid Science 11 Pages: 26-31
  • Peer Reviewed
• [Journal Article] Selective Binding of Carotenoids with a Shorter Conjugated Chain to the LH2 Antenna Complex and those with a Longer Conjugated Chain to the Reaction Center from Rubrivivax gelatinosus (2007)
  • Author(s): Y. Kakitani, R. Fujii, Y. Hayakawa, M. Kurahashi, Y. Koyama, J. Harada, K. Shimada
  • Journal Title: Biochemistry 46 Pages: 7302-7313
  • Peer Reviewed
• [Journal Article] Symmetry Control of Radiative Decay in Linear Polyenes : Low Barriers for Isomerization in the S1 State of Hexadecaheptaene (2007)
  • Author(s): R. L. Christensen, M. G. I. Galinato, E. F. Chu, R. Fujii, H. Hashimoto, H. A. Frank
  • Journal Title: J. Am. Chem. Soc 129(6) Pages: 1769-1775
  • Peer Reviewed
• [Journal Article] Triplet States of Carotenoids Having Shorter Conjugation Lengths Reconstituted into the Reaction Centre of Rhodobacter sphaeroides R26.1 Detected by Time-Resolved Absorption Spectroscopy (2006)
  • Author(s): R. Fujii, M. Shimizu, T. Nishio, T. Kusumoto, D. Niedzwiedzki, Z. Pendon, H. A. Frank, R. J. Cogdell, H. Hashimoto
  • Journal Title: Carotenoid Science 10 Pages: 72-77
  • Peer Reviewed
• [Journal Article] Structures and Functions of Carotenoids Bound to Reaction Centres from Purple Photosynthetic Bacteria (2006)
  • Author(s): H. Hashimoto, R. Fujii, K. Yanagi, T. Kusumoto, A. T. Gardiner, A. W. Roszak, N. W. Issacs, Z. Pendon, D. Niedzwiedski, H. A. Frank, R. J. Cogdell
  • Journal Title: Pure Appl. Chem 78(8) Pages: 1505-1518
  • Peer Reviewed
• [Journal Article] Triplet-State Conformational Changes in 15-Cis-Spheroidene Bound to the Reaction Center from Rhodobacter sphaeroides 2.4.1 as Revealed by Time-Resolved EPR Spectroscopy : Strengthened Hypothetical Mechanism of Triplet-Energy Dissipation (2006)
  • Author(s): Y. Kakitani, R. Fujii, Y. Koyama, H. Nagae, L. Walker, B. Salter, A. Angerhofer
  • Journal Title: Biochemistry 45(7) Pages: 2053-2062
  • Peer Reviewed
• [Presentation] オキナワモズク盤状体由来のフコキサンチン-クロロフィルa/c蛋白質(FCP)の単離 (2009)
  • Author(s): 喜多麻美子,藤井律子,伊波匡彦,橋本秀樹
  • Organizer: 第50回日本植物生理学会
  • Place of Presentation: 名古屋大学(名古屋市) , ポスター
  • Year and Date: 20090321-24
• [Presentation] Structure of Reaction Centre Carotenoid in Blastochloris viridis (2008)
  • Author(s): A. W. Roszak, A. T. Gardiner, V. Moulisova, A. D. Putranto, R. Fujii, H. Hashimoto, N. W. Isaacs, R. J. Cogdell
  • Organizer: 第22回カロテノイド研究談話会
  • Place of Presentation: ホテルムーンビーチ(沖縄),招待講演
  • Year and Date: 20080628-29
• [Presentation] Structure of the Carotenoid Bound to the Reaction Centre from Blastochloris viridis (2008)
  • Author(s): A. W. Roszak, A. T. Gardiner, V. Moulisova, A. D. Putranto, R. Fujii, H. Hashimoto, N. W. Isaacs, R. J. Cogdell
  • Organizer: The 15th International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan,
  • Year and Date: 20080622-27
• [Presentation] Femtosecond Time-Resolved Absorption Spectra of Photosynthetic Membranes from a Purple Bacterium Blastochloris (Rhodopseudomonas) viridis (2008)
  • Author(s): R. Fujii, N. Wakatake, S. Shimonaka, Q.-D. Chen, M. Sugisaki, A. T. Gardiner, R. J. Cogdell, H. Hashimoto
  • Organizer: 15th International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan
  • Year and Date: 20080622-27
• [Presentation] Construction of Hybrid Photosynthetic Units using Peripheral and Core Antennae from Two Different Species of Photosynthetic Bacteria : Detection of the Energy Transfer from Bacteriochlorophyll a in LH2 to Bacteriochlorophyll b in LH1 (2008)
  • Author(s): R. Fujii, S. Shimonaka, N. Uchida, A. T. Gardiner, R. J. Cogdell, M. Sugisaki and H. Hashimoto
  • Organizer: 15th International Symposium on Carotenoids
  • Place of Presentation: Hotel Moon Beach, Okinawa, Japan
  • Year and Date: 20080622-27
• [Presentation] Construction of Hybrid Photosynthetic Units using Peripheral and Core Antennae from Two Different Species of Photosynthetic Bacteria : Detection of the Energy Transfer from Bacteriochlorophyll a in LH2 to Bacteriochlorophyll b in LH1 (2007)
  • Author(s): R. Fujii, S. Shimonaka, N. Uchida, A. T. Gardiner, R. J. Cogdell, M. Sugisaki and H. Hashimoto
  • Organizer: 14th International Congress of Photosynthesis : Satellite Workshop on Photosynthetic Light-Harvesting Systems
  • Place of Presentation: Buchanan Arms Hotel, Drymen, UK
  • Year and Date: 20070718-19
• [Presentation] 人工膜内に配列した異種の光合成色素蛋白複合体間のエネルギー伝達 (2007)
  • Author(s): 藤井律子,下中奨三,内田直子,A. T. Gardiner,R. J. Cogdell,杉崎満,橋本秀樹
  • Organizer: 日本物理学会2007年春季大会
  • Place of Presentation: 鹿児島大学郡元キャンパス(鹿児島市)
  • Year and Date: 20070318-21
• [Presentation] 人工膜内に配列した異種の光合成色素蛋白複合体間のエネルギー伝達 (2006)
  • Author(s): 藤井律子,下中奨三,内田直子,A. T.Gardiner,R. J. Cogdell,杉崎満,橋本秀樹
  • Organizer: 第17 回光物性研究会
  • Place of Presentation: 大阪市立大学(大阪)
  • Year and Date: 20061208-09
• [Presentation] Heterogeneous Two-Dimensional-Crystallization and Energy Transfer of the LH2 and LH1-RC Complexes from Different Species of Photosynthetic Bacteria : An Investigation by Means of Electron Microscopy and Near-Infrared Fluorescence Spectroscopy (2006)
  • Author(s): R. Fujii, S. Shimonaka, N. Uchida, A. T. Gardiner, R. J. Cogdell, M. Sugisaki And H. Hashimoto
  • Organizer: 12th International Symposium of Phototrophic Prokaryotes
  • Place of Presentation: Pau(south of France)
  • Year and Date: 20060827-0901
• [Book] In : Petra Fromme (Ed.), Photosynthetic Protein Complexes A Structural Approach (2008)
  • Author(s): R. J. Cogdell, A. T. Gardiner, M. Gabrielsen, J. Southall, A. W. Roszak, N. W. Isaacs, R. Fujii and H. Hashimoto, Wiley-VCH Verlag GmbH & Co. KGaA,
  • Total Pages: 325-340
  • Publisher: The Structure of Purple Bacterial Antenna Complexes
• [Book] 光合成微生物の機能と応用(上原赫編)1章2節 (2006)
  • Author(s): 橋本秀樹,藤井律子
  • Total Pages: 18-37
  • Publisher: (株)シーエムシー出版,「光合成の初期過程の最新の描像」
• [Book] 光物性の基礎と応用(光物性研究会組織委員会編)第3部5章 (2006)
  • Author(s): 橋本秀樹,藤井律子,杉崎満
  • Total Pages: 239-261
  • Publisher: オプトロニクス社,「有機・バイオ-光合成アンテナ色素淡白複合体・カロテノイド・超高速レーザー分光-」
• [Book] In : B. Grimm, R. Porra, W. Rudiger and H. Scheer (eds.), Chlorophylls and Bacteriochlorophylls : Biochemistry, Biophysics, Functions and Applications (Advances in Photosynthesis and Respiration, Vol. 25) (2006)
  • Author(s): Y. Koyama, Y. Kakitani, L. Limantara and R. Fujii, Springer
  • Total Pages: 323-335
  • Publisher: Effects of Axial Coordination, Electronic Excitation and Oxidation on Bond Orders in the Bacteriochlorin Macrocycle, and Generation of Radical Cation on Photo-Excitation of in vitro and in vivo Bacteriochlorophyll a Aggregates : Resonance Raman Study