2008 Fiscal Year Final Research Report
Molecular Mechanism of Photoprotective Function of a Carotenoid Bound to the Photoreaction Center Revealed by Time-Resolved X-Ray Crystallography
Project/Area Number |
18684016
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Condensed matter physics I
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Research Institution | Osaka City University |
Principal Investigator |
FUJII Ritsuko Osaka City University, 大学院・理学研究科, 博士研究員 (80351740)
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Project Period (FY) |
2006 – 2008
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Keywords | 光物性 / 光合成 / たんぱく質構造 / 物性 / 光誘起協力現象 / エネルギー散逸 |
Research Abstract |
紅色光合成細菌Rhodobacter sphaeroides 2.4.1由来の光反応中心複合体の1.95Åの高分解能X線結晶構造解析に日本で初めて成功した。その結果、光合成光保護メカニズムに関与すると考えられるカロテノイドの結合長の変位を検出するためには2.5Å以上の高分解能が必須であることがわかった。また分解能を左右するクライオプロテクションなどの操作を改良することにより、従来よりも小さい光を透過する結晶でも高分解能を得ることに成功した。
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Research Products
(20 results)
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[Journal Article] Structures and Functions of Carotenoids Bound to Reaction Centres from Purple Photosynthetic Bacteria2006
Author(s)
H. Hashimoto, R. Fujii, K. Yanagi, T. Kusumoto, A. T. Gardiner, A. W. Roszak, N. W. Issacs, Z. Pendon, D. Niedzwiedski, H. A. Frank, R. J. Cogdell
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Journal Title
Pure Appl. Chem 78(8)
Pages: 1505-1518
Peer Reviewed
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[Presentation] Structure of the Carotenoid Bound to the Reaction Centre from Blastochloris viridis2008
Author(s)
A. W. Roszak, A. T. Gardiner, V. Moulisova, A. D. Putranto, R. Fujii, H. Hashimoto, N. W. Isaacs, R. J. Cogdell
Organizer
The 15th International Symposium on Carotenoids
Place of Presentation
Hotel Moon Beach, Okinawa, Japan,
Year and Date
20080622-27
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[Book] In : B. Grimm, R. Porra, W. Rudiger and H. Scheer (eds.), Chlorophylls and Bacteriochlorophylls : Biochemistry, Biophysics, Functions and Applications (Advances in Photosynthesis and Respiration, Vol. 25)2006
Author(s)
Y. Koyama, Y. Kakitani, L. Limantara and R. Fujii, Springer
Total Pages
323-335
Publisher
Effects of Axial Coordination, Electronic Excitation and Oxidation on Bond Orders in the Bacteriochlorin Macrocycle, and Generation of Radical Cation on Photo-Excitation of in vitro and in vivo Bacteriochlorophyll a Aggregates : Resonance Raman Study