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2008 Fiscal Year Final Research Report

Development of novel methods for efficient construction of functional RNAs

Research Project

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Project/Area Number 18685020
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Chemistry related to living body
Research InstitutionKyushu University

Principal Investigator

IKAWA Yoshiya  Kyushu University, 大学院・工学研究院, 准教授 (70281087)

Project Period (FY) 2006 – 2008
Keywords生体関連高分子化学 / 進化工学
Research Abstract

「人工酵素などの機能性生体高分子のテーラーメイドな創製」は、RNAに限らず、広く生体高分子研究において、基礎化学/生物学的に、また医療やバイオ工学などへの応用面からも重要な課題である。本研究では、研究代表者の考案した新手法「design & selection法」を基盤とする人工リボザイムの創製、機能改変、機能解析に取り組み、本手法の確立を行い、その有効性を示した。

  • Research Products

    (29 results)

All 2008 2007 2006 Other

All Journal Article (9 results) Presentation (18 results) Book (1 results) Remarks (1 results)

  • [Journal Article] N-confused porphyrin possessing glucamine-appendants : aggregation and acid/base properties in aquaous media2008

    • Author(s)
      Ikawa. Y., Ogawa, H., Harada, H., & Furuta, H
    • Journal Title

      Bioorg. Med. Chem. Lett 18

      Pages: 6394-6397

  • [Journal Article] Acid-base properties and DNA-binding of water soluble N-confused porphyrins with cationic side-arms2008

    • Author(s)
      Ikawa. Y., Moriyama, S., Harada, H., & Furuta, H
    • Journal Title

      Org. Biomol. Chem 6

      Pages: 4157-4166

  • [Journal Article] Mutation analysis of the base-pair connecting two functional modules in the DSL ribozyme2008

    • Author(s)
      Ishikawa, J., Furuta. H., & Ikawa, Y.
    • Journal Title

      Nucleic Acids Symp. Ser 52

      Pages: 523-524

  • [Journal Article] Facile syntheses of BODIPY derivatives for fluorescent labeling of the 5' and 3' ends of RNAs2008

    • Author(s)
      Ikawa. Y., Moriyama S., & Furuta, H
    • Journal Title

      Anal. Biochem 378

      Pages: 166-170

  • [Journal Article] Selection of a novel class of RNA-RNA interaction motifs based on the ligase ribozyme with defined modular architecture2008

    • Author(s)
      Ohuchi, SP., Ikawa, Y., & Nakamura Y.
    • Journal Title

      Nucleic Acids Res 36

      Pages: 3600-3607

  • [Journal Article] Design and analysis of a structural RNA that acts as a template for peptide ligation2007

    • Author(s)
      Kashiwagi, N., Furuta. H., & Ikawa, Y
    • Journal Title

      Nucleic Acids Symp. Ser 51

      Pages: 387-388

  • [Journal Article] Synthesis of a water soluble N-confused porphyrin and its interaction with nucleic acids2007

    • Author(s)
      Moriyama, S., Ikawa. Y., & Furuta, H
    • Journal Title

      Nucleic Acids Symp. Ser 51

      Pages: 207-208

  • [Journal Article] 人工RNA触媒創製の新手法 : de novoデザインと進化分子工学の融合.2006

    • Author(s)
      Ikawa, Y.
    • Journal Title

      蛋白質核酸酵素 51

      Pages: 950-957

  • [Journal Article] Construction of an artificial ribozyme which ligates an RNA fragment activated by nicotinamide mononucleotide2006

    • Author(s)
      Fujita, Y., Furuta. H., & Ikawa, Y
    • Journal Title

      Nucleic Acids Symp. Ser 50

      Pages: 349-350

  • [Presentation] ユニット集積型人工リガーゼ・リボザイムの構造・機能相関解析2008

    • Author(s)
      Yuki Fujita, Hiroyuki Furuta, Yoshiya Ikawa
    • Organizer
      第22回生体機能関連化学シンポジウム
    • Place of Presentation
      東北大学
    • Year and Date
      20080928-29
  • [Presentation] High Turnover Ability of a Trans-Acting RNA Ligase Ribozyme Developed through Modular Engineering2008

    • Author(s)
      Junya Ishikawa, Hiroyuki Furuta, Yoshiya Ikawa
    • Organizer
      Thirteenth Annual Meeting of the RNA Society
    • Place of Presentation
      Berlin
    • Year and Date
      20080728-0803
  • [Presentation] Tailoring RNA Modular Units on a Common Scaffold : A Modular Ribozyme Having a Catalytic Unit for b-NMN Activated RNA Ligation2008

    • Author(s)
      Yuki Fujita, Hiroyuki Furuta, Yoshiya Ikawa
    • Organizer
      Thirteenth Annual Meeting of the RNA Society
    • Place of Presentation
      Berlin
    • Year and Date
      20080728-0803
  • [Presentation] RNA Science & Technology through Design & Evolution2008

    • Author(s)
      Yoshiya Ikawa
    • Organizer
      Prof. Jean-Marie Lehn Symposium III
    • Place of Presentation
      Nishijin Plaza, Fukuoka, Janan
    • Year and Date
      2008-10-17
  • [Presentation] RNAとペプチドのcross-catalysisモデル構築へ向けた試み2008

    • Author(s)
      Norimasa Kashiwagi, Hiroyuki Furuta, Yoshiya Ikawa
    • Organizer
      第10回生命化学研究会シンポジム
    • Place of Presentation
      熊本
    • Year and Date
      2008-01-11
  • [Presentation] デザイン型RNA上でRNP複合体形成を介する効率的ペプチド連結反応2007

    • Author(s)
      Norimasa Kashiwagi, Hiroyuki Furuta, Yoshiya Ikawa
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学学会大会・合同大会
    • Place of Presentation
      横浜
    • Year and Date
      20071211-15
  • [Presentation] モジュール工学によるDSLリボザイムの構造・活性相関の解析2007

    • Author(s)
      Junya Ishikawa, Shohei Kuramitsu, Hiroyuki Furuta, Tan Inoue, Yoshiya Ikawa
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学学会大会・合同大会
    • Place of Presentation
      横浜
    • Year and Date
      20071211-15
  • [Presentation] 小型化と高性能化に向けたユニット集積型人工リガーゼ・リボザイムの構造解析2007

    • Author(s)
      Yuki Fujita, Hiroyuki Furuta, Yoshiya Ikawa
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学学会大会・合同大会
    • Place of Presentation
      横浜
    • Year and Date
      20071211-15
  • [Presentation] Design and analysis of a structural RNA that acts as a template for peptide ligation2007

    • Author(s)
      Norimasa Kashiwagi, Hiroyuki Furuta, Yoshiya Ikawa
    • Organizer
      5th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      Tokyo
    • Year and Date
      20071120-22
  • [Presentation] Junya Ishikawa, Shohei Kuramitsu, Hiroyuki Furuta, Tan Inoue, Yoshiya Ikawa2007

    • Author(s)
      テトラループ・レセプター・ユニットを標的としたDSLリボザイムのモジュール工学
    • Organizer
      第9回日本RNA学会年会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      20070728-31
  • [Presentation] ユニット集積型人工リガーゼ・リボザイムの構造・機能相関解析2007

    • Author(s)
      Yuki Fujita, Hiroyuki Furuta, Yoshiya Ikawa
    • Organizer
      第9回日本RNA学会年会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      20070728-31
  • [Presentation] RNP形成によりペプチド連鎖反応を促進するデザイン型RNA構造体の作成と解析2007

    • Author(s)
      Norimasa Kashiwagi, Hiroyuki Furuta, Yoshiya Ikawa
    • Organizer
      第9回日本RNA学会年会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      20070728-31
  • [Presentation] Modular Design & Evolution of RNA/RNP Catalysts2007

    • Author(s)
      Yoshiya Ikawa, Norimasa Kashiwagi, Yuki Fujita, Hiroyuki Furuta
    • Organizer
      Synthetic Biology 3.0
    • Place of Presentation
      Zurich, Switzerland
    • Year and Date
      20070624-26
  • [Presentation] Toward Construction of Mg2+-Free Catalytic Modules for RNA Ligase Ribozymes2007

    • Author(s)
      Kuniyasu Kumon, Hiroyuki Furuta, Yoshiya Ikawa
    • Organizer
      12th Annual Meeting of the RNA Society
    • Place of Presentation
      Madison, Wisconsin
    • Year and Date
      20070529-0603
  • [Presentation] Effective Peptide Ligation on 3D-RNA through Formation of a Ribonucleo-Peptide Complex2007

    • Author(s)
      Norimasa Kashiwagi, Hiroyuki Furuta, Yoshiya Ikawa
    • Organizer
      12th Annual Meeting of the RNA Society
    • Place of Presentation
      Madison, Wisconsin
    • Year and Date
      20070529-0603
  • [Presentation] A Novel RNA Catalyzing RNA-RNA Ligation Activated by β-NMN2007

    • Author(s)
      Yuki Fujita, Hiroyuki Furuta, Yoshiya Ikawa
    • Organizer
      12th Annual Meeting of the RNA Society
    • Place of Presentation
      Madison, Wisconsin
    • Year and Date
      20070529-0603
  • [Presentation] Y. Fujita, Y. Ikawa, H. Furuta2006

    • Author(s)
      A novel RNA enzyme catalyzing RNA-RNA ligation activated by b-NMN
    • Organizer
      RNA 2006
    • Place of Presentation
      Izu
    • Year and Date
      20061203-07
  • [Presentation] Effective peptide ligation on a 3D-RNA through formation of a ribonucleo-peptide complex2006

    • Author(s)
      N. Kashiwagi, Y. Ikawa, H. Furuta
    • Organizer
      RNA 2006
    • Place of Presentation
      Izu
    • Year and Date
      20061203-07
  • [Book] ed. Silverman, SK., Landes Bioscience2006

    • Author(s)
      Inoue, T., & Ikawa, Y
    • Total Pages
      37-47
    • Publisher
      Protein switched ribozymes. in Nucleic Acid Switches and Sensors
  • [Remarks]

    • URL

      http://www.cstf.kyushu-u.ac.jp/~furutalab/

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Published: 2010-06-10   Modified: 2016-04-21  

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