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2008 Fiscal Year Final Research Report

Development of detection technology for electrostatic potential of single biomolecule

Research Project

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Project/Area Number 18686059
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Material processing/treatments
Research InstitutionNagoya University

Principal Investigator

SAITO Nagahiro  Nagoya University, 大学院・工学研究科, 准教授 (00329096)

Project Period (FY) 2006 – 2008
Keywords表面・界面制御 / 生体分子
Research Abstract

本研究では、走査型プローブ顕微鏡および光導波路分光法を用いて、異なる化学的特性(はっ水性・親水性)を有する基材表面における生体1分子の吸着挙動の解明を行った。生体分子には、DNAおよびヒト血漿フィブリノーゲン(HPF)をモデルターゲットに用いた。また、ケルビン力顕微鏡およびゼータ電位測定により、これら生体分子の静電ポテンシャルを明らかにした。これらの成果は将来のバイオデバイス作製に向けての基盤技術となる。

  • Research Products

    (46 results)

All 2009 2008 2007 2006

All Journal Article (20 results) (of which Peer Reviewed: 18 results) Presentation (19 results) Book (3 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 1 results)

  • [Journal Article] Control of site-selective adsorption reaction on a biomimetic super-hydrophilic /super-hydrophobic micropatterned template2008

    • Author(s)
      T. Ishizaki, H. Sakurai, N. Saito, O. Takai
    • Journal Title

      Surf. Coat. Technol. 202

      Pages: 5535-5538

    • Peer Reviewed
  • [Journal Article] Scanning probe microscopy for the analysis of composite Ti/hydrocarbon plasma polymer thin films2008

    • Author(s)
      A. Choukourov, A. Grinevich, D. Slavinska, H. Biederman, N. Saito, O. Takai
    • Journal Title

      Surf. Sci. 602

      Pages: 1011-1019

    • Peer Reviewed
  • [Journal Article] Effect of Humidity and Solution Age on the Growth of Organosilane Self-Assembled Monolayers2008

    • Author(s)
      S.H. Lee, T. Ishizaki, N. Saito, O. Takai
    • Journal Title

      J. Jpn. Appl. Phys. 47

      Pages: 6416-6421

    • Peer Reviewed
  • [Journal Article] Surface Characterization on Binary Nano/Micro Domain Composed of Alkyl and Amino-terminated Self-assembled Monolayer2008

    • Author(s)
      S.H. Lee, T. Ishizaki, N. Saito, O. Takai
    • Journal Title

      Appl. Surf. Sci. 254

      Pages: 7453-7458

    • Peer Reviewed
  • [Journal Article] Effect of Reaction Temperature on Growth of Organosilane Self-Assembled Monolayer2008

    • Author(s)
      S.H. Lee, T. Ishizaki, N. Saito, O. Takai
    • Journal Title

      J. Jpn. Appl. Phys. 47

      Pages: 6442-6447

  • [Journal Article] Comparative study of the molecular aggregation state of alkyl organic monolayers prepared on Si and hydrogen-terminated Si substrates2008

    • Author(s)
      T. Ishizaki, S.H. Lee, N. Saito, O. Takai
    • Journal Title

      Nanotehnology 19

      Pages: 055601-055607

    • Peer Reviewed
  • [Journal Article] Application of ultra-water-repellent surface to cell culture2007

    • Author(s)
      K. Ino, A. Ito, Y.Y. Wu, N. Saito, E. Hibino, O. Takai, H. Honda
    • Journal Title

      J. Bioscience. Bioengineering 104

      Pages: 420-423

    • Peer Reviewed
  • [Journal Article] A micropatterned carbohydrate display for tissue engineering by self-assembly of heparin2007

    • Author(s)
      H. Sato, Y. Miura, N. Saito, K. Kobayashi, O. Takai
    • Journal Title

      Surf. Sci. 601

      Pages: 3871-3875

    • Peer Reviewed
  • [Journal Article] Localized surface plasmon resonance of silicon compounds adsorbed on silver nanoparticles2007

    • Author(s)
      M.A. Bratescu, N. Saito, H. Mori, O. Takai
    • Journal Title

      Surf. Sci. 601

      Pages: 3886-3891

    • Peer Reviewed
  • [Journal Article] Probing into Adsorption Behavior of Human Plasma Fibrinogen on Self-Assembled Monolayers with Different Chemical Properties by Scanning Probe Microscopy2007

    • Author(s)
      T. Ishizaki, N. Saito, Y. Sato, O. Takai
    • Journal Title

      Surf. Sci. 601

      Pages: 3861-3865

    • Peer Reviewed
  • [Journal Article] SPM analysis of fibrinogen adsorption on solid surfaces2007

    • Author(s)
      A. Choukourov, A. Grinevich, N. Saito, O. Takai
    • Journal Title

      Surf. Sci. 601

      Pages: 3948-3951

    • Peer Reviewed
  • [Journal Article] Electrochemical soft lithography of an 1,7-octadiene monolayer covalently linked to hydrogen-terminated silicon using scanning probe microscopy2007

    • Author(s)
      S.H. Lee, T. Ishizaki, N. Saito, O. Takai
    • Journal Title

      Surf. Sci. 601

      Pages: 4206-4211

    • Peer Reviewed
  • [Journal Article] Local generation of carboxyl groups on an organic monolayer through chemical conversion using scanning probe anodization2007

    • Author(s)
      S.H. Lee, T. Ishizaki, N. Saito, O. Takai
    • Journal Title

      Mater. Sci. Eng., C 27

      Pages: 1241-1246

    • Peer Reviewed
  • [Journal Article] Patterned hydrophobic-hydrophilic templates made from microwave-plasma enhanced chemical vapor deposited thin films2007

    • Author(s)
      Y.Y. Wu, M. Kouno, N. Saito, F.A. Nae, Y. Inoue, O. Takai
    • Journal Title

      Thin Solid Films 515

      Pages: 4203-4208

    • Peer Reviewed
  • [Journal Article] A micropatterned multifunctional carbohydrate display by an orthogonal self-assembling strategy2007

    • Author(s)
      H. Sato, Y. Miura, N. Saito, K. Kobayashi, O. Takai
    • Journal Title

      Biomacromolecules 8

      Pages: 753-756

    • Peer Reviewed
  • [Journal Article] The Importance of Precursor Molecules Symmetry in the Formation of Self-assembled Monolayers2007

    • Author(s)
      S.H. Lee, N. Saito, O. Takai
    • Journal Title

      Jpn. J. Appl. Phys., Part 1 46

      Pages: 1118-1123

  • [Journal Article] Visualization of human plasma fibrinogen adsorbed on highly oriented pyrolytic graphite by scanning probe microscopy2006

    • Author(s)
      R. Ohta, N. Saito, T. Ishizaki, O. Takai
    • Journal Title

      Surf. Sci. 600

      Pages: 1674-1678

    • Peer Reviewed
  • [Journal Article] Treatment of Immobilized Collagen on Poly(tetrafluoroethylene) Nanoporous Membrane with Plasma2006

    • Author(s)
      M.A. Bratescu, N. Saito, O. Takai
    • Journal Title

      Jpn. J. Appl. Phys. 45

      Pages: 8352-8357

    • Peer Reviewed
  • [Journal Article] Carbohydrate microarrays by click chemistry2006

    • Author(s)
      H. Sato, Y. Miura, T. Yamauchi, N. Saito, K. Kobayashi, O. Takai
    • Journal Title

      Trans. Mater. Research. Soc. 31

      Pages: 659-703

    • Peer Reviewed
  • [Journal Article] Study of alkyl organic monolayers with different alkyl chain lengths directly attached to silicon2006

    • Author(s)
      T. Ishizaki, N. Saito, S.H. Lee, K. Ishida, O. Takai
    • Journal Title

      Langmuir 22

      Pages: 9962-9966

    • Peer Reviewed
  • [Presentation] 超はっ水/PEGマイクロテンプレートを用いた微生物の位置選択的固定化2009

    • Author(s)
      樋口晶生, 宮原康弘, 齋藤永宏, 高井 治
    • Organizer
      日本金属学会2009年春期(第114回)講演大会
    • Place of Presentation
      東京,日本
    • Year and Date
      2009-03-26
  • [Presentation] 疎水性及び親水性二元系SAMの濡れ性の評価2009

    • Author(s)
      鈴木和也, 李 先炯, 齋藤永宏, 高井 治
    • Organizer
      日本金属学会2009年春期(第114回)講演大会
    • Place of Presentation
      東京,日本
    • Year and Date
      2009-03-26
  • [Presentation] Adsorption of protein on polyelectrolyte brush surface2009

    • Author(s)
      A. Higuchil, H. Tatematsu, T. Fujima, N. Saito, O. Takai
    • Organizer
      Spring 2009 National Meeting & Exposition
    • Place of Presentation
      Salt Lake City, U.S.A.
    • Year and Date
      2009-03-23
  • [Presentation] Chemical dynamics of protein adsorption and desorption on regulated surfaces in aqueous solutions2009

    • Author(s)
      N. Saito, J. Hieda, O. Takai
    • Organizer
      Spring 2009 National Meeting & Exposition
    • Place of Presentation
      Salt Lake City, U.S.A.
    • Year and Date
      2009-03-22
  • [Presentation] Added Salt Effect on a Fibrinogen Immobilization Kinetics at a Polelectrolyte Brush2009

    • Author(s)
      T. Fujima, M. Matsuno, M. Bratescu, N. Saito, O. Takai
    • Organizer
      Ninth International Symposium on Biomimetic Materials Processing(BMMP-9)
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2009-01-21
  • [Presentation] Added Salt Effect on a Fibrinogen Adsorption Kinetics at a Planer Strong Polyelectrolyte Brush2008

    • Author(s)
      T. Fujima, M. Matsuno, M. A. Bratescu, N. Saito, O. Takai
    • Organizer
      IUMRS-ICA 2008
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2008-12-11
  • [Presentation] The Influence of Physicochemical Properties to Bacterial Adsorption to Material Surfaces2008

    • Author(s)
      Y. Miyahara, N. Saito, O. Takai
    • Organizer
      IUMRS-ICA 2008
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2008-12-10
  • [Presentation] UV-ATR Polarization Spectroscopy of Self-Assembled Coumarin Monolayer2008

    • Author(s)
      M.A. Bratescu, D.B. Allred, N. Saito, M, Sarikaya, O. Takai
    • Organizer
      IUMRS-ICA 2008
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2008-12-10
  • [Presentation] Study of Protein Adsorption onto a Polymer Film by in-situ UV Attenuated Total Reflectance Spectroscopy2008

    • Author(s)
      M.A. Bratescu, S. Fujita, N. Saito, O. Takai
    • Organizer
      MRS fall meeting 2008
    • Place of Presentation
      Boston, USA
    • Year and Date
      2008-12-01
  • [Presentation] Kinetic Study of Protein Adsorption on Polyelectrolyte Brush Surface2008

    • Author(s)
      H. Tatematsu, T. Fujima, N. Saito, O. Takai
    • Organizer
      AVS 55th International Symposium and Exhibition
    • Place of Presentation
      Boston, USA
    • Year and Date
      2008-10-21
  • [Presentation] Fabrication of Micro-Templates for the Control of Bacterial Immobilization2008

    • Author(s)
      Y. Miyahara, N. Saito, O. Takai
    • Organizer
      AVS 55th International Symposium and Exhibition
    • Place of Presentation
      Boston, USA
    • Year and Date
      2008-10-21
  • [Presentation] 微生物付着制御のためのマイクロテンプレートの作製2008

    • Author(s)
      稗田純子, 宮原康弘, 齋藤永宏, 高井 治
    • Organizer
      第57回高分子討論会
    • Place of Presentation
      大阪市立大学,日本
    • Year and Date
      2008-09-25
  • [Presentation] 微生物吸着挙動に及ぼす物理化学的表面特性の影響2008

    • Author(s)
      宮原康弘, 齋藤永宏, 高井 治
    • Organizer
      第118回講演大会(主催 : 表面技術協会)
    • Place of Presentation
      近畿大学,日本
    • Year and Date
      2008-09-02
  • [Presentation] In-situ Study of Bacterial S-layer Protein Binding Process with Noble Metal Surface2008

    • Author(s)
      M. A. Bratescu, D.B. Allred, N. Saito, M. Sarikaya, O. Takai
    • Organizer
      Interfinish 2008
    • Place of Presentation
      釜山,韓国
    • Year and Date
      2008-06-17
  • [Presentation] Adhesion Control of Bacteria with Micropatterned Super-Hydrophobic/Super-Hydrophilic and Super-Hydrophobic/Poly(Ethelene Glycol) Surfaces2008

    • Author(s)
      Y. Miyahara, T. Ishizaki, N. Saito, O. Takai
    • Organizer
      Interfinish 2008,
    • Place of Presentation
      釜山,韓国
    • Year and Date
      2008-06-17
  • [Presentation] 高分子電解質ブラシ上へのタンパク質捕捉キネティクスの添加塩濃度依存性価2008

    • Author(s)
      藤間卓也, 松野正幸, 齋藤永宏, 高井 治
    • Organizer
      2008年春季第55回応用物理学関係連合講演会
    • Place of Presentation
      東京,日本
    • Year and Date
      2008-03-29
  • [Presentation] 固体表面形状と細菌付着の関係2008

    • Author(s)
      宮原康弘, 石崎貴裕, 齋藤永宏, 高井 治
    • Organizer
      2008年春季第55回応用物理学関係連合講演会
    • Place of Presentation
      東京,日本
    • Year and Date
      2008-03-29
  • [Presentation] 機能性有機分子膜上でのヒト血漿フィブリノーゲン吸着挙動の解析と制御2008

    • Author(s)
      松野正幸, 藤間卓也, Maria A. Bratescu, 石崎貴裕, 齋藤永宏, 高井 治
    • Organizer
      表面技術協会第117回講演大会
    • Place of Presentation
      東京,日本
    • Year and Date
      2008-03-12
  • [Presentation] In-situ Study of the Bacterial S-layer Protein Adsorption by Attenuated Total Reflectance Spectroscopy2008

    • Author(s)
      M.A. Bratescu, D.B. Allred, N.Saito, O. Takai
    • Organizer
      Eighth International Symposium on Biomimetic Materials Processing(BMMP-8)
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2008-01-23
  • [Book] I-1.6 自己組織化とその利用, I-4.6 化学特性, 薄膜ハンドブック2008

    • Author(s)
      齋藤永宏, 藤間卓也, 高井治
    • Total Pages
      92-200, 508-522
    • Publisher
      オーム社
  • [Book] 自己組織化単分子膜を利用した分子メモリの作製, 自然に学ぶ材料プロセッシング2007

    • Author(s)
      齋藤永宏, 石崎貴裕
    • Total Pages
      216-219
    • Publisher
      三協出版
  • [Book] 超はっ水性および超親水性表面を用いた新しい細胞培養技術2007

    • Author(s)
      高井 治, 石崎貴裕, 齋藤永宏
    • Total Pages
      225-231
    • Publisher
      エヌ・ティー・エス
  • [Patent(Industrial Property Rights)] 自己組織化単分子膜の作製方法とその利用2008

    • Inventor(s)
      齋藤 永宏, 石崎 貴裕, 高井 治
    • Industrial Property Rights Holder
      国立大学法人名古屋大学
    • Industrial Property Number
      特許権,特許第4065962号
    • Acquisition Date
      2008-01-18
  • [Patent(Industrial Property Rights)] 自己組織化単分子膜の作製法とその利用2006

    • Inventor(s)
      齋藤 永宏, 石崎 貴裕, 高井 治
    • Industrial Property Rights Holder
      国立大学法人名古屋大学
    • Industrial Property Number
      特許権,PCT/JP2006/307962
    • Filing Date
      2006-04-15
    • Overseas
  • [Patent(Industrial Property Rights)] 自己組織化単分子膜の作製法2006

    • Inventor(s)
      齋藤 永宏, 石崎 貴裕, 高井 治
    • Industrial Property Rights Holder
      国立大学法人名古屋大学
    • Industrial Property Number
      特許権,特許出願2006-218265
    • Filing Date
      2006-08-10
  • [Patent(Industrial Property Rights)] 自己組織化単分子膜作製装置とその利用2006

    • Inventor(s)
      齋藤 永宏, 石崎 貴裕, 高井 治
    • Industrial Property Rights Holder
      国立大学法人名古屋大学
    • Industrial Property Number
      特許権,特許出願2006-352281
    • Filing Date
      2006-12-27

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Published: 2010-06-10   Modified: 2016-04-21  

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