Secreted peptidomics focused on posttransiational modifications

2008 Fiscal Year Final Research Report

• Project/Area Number: 18687003
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: 植物生理・分子
• Research Institution: Nagoya University
• Principal Investigator: MATSUBAYASHI Yoshikatsu Nagoya University, 大学院・生命農学研究科, 准教授 (00313974)
• Project Period (FY): 2006 – 2008
• Keywords: 分泌型ペプチド / ペプチドホルモン / 翻訳後修飾

Research Abstract

植物のかたちづくりには様々な内生のペプチドホルモンが関与している.本研究では,in silicoおよび生化学的解析を統合して,翻訳後修飾ペプチドホルモン候補を探索し,その生理機能解梼を行なった.その結果,複数の新規ペプチドホルモン候補を見出すことに成功した,また,翻訳後修飾のひとつであるチロシン硫酸化酵素の精製とクローニングを行ない,その遺伝子破壊株が様々な成長異常を示すことを見出した.

Research Products

• [Journal Article] Identification of tyrosylprotein sulfotransferase in Arabidopsis. (2009)
  • Author(s): Komori, R., Amano, Y., Ogawa-Ohnishi, M., Matsubayashi, Y.
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 15067-15072
• [Journal Article] A glycopeptide regulating stem cell fate in Arabidopsis thaliana. (2009)
  • Author(s): Ohyama, K., Shinohara, H., Ogawa-Ohnishi, M., Matsubayashi, Y.
  • Journal Title: Nature Chem Biol 5 Pages: 578-580
• [Journal Article] Arabidopsis CLV3 peptide directly binds CLV1 ectodomain. (2008)
  • Author(s): Ogawa, M., Shinohara, H., Sakagami, Y., Matsubayashi, Y.
  • Journal Title: Science 319 Pages: 294
• [Journal Article] Non-cell-autonomous control of vascular stein cell fate by a CLE peptide/receptor system. (2008)
  • Author(s): Hirakawa, Y., Shinohara, H., Kondo, Y., Inoue, A., Nakanomyo, I., Ogawa, M., Sawa, S., Ohashi-Ito, K., Matsubayashi, Y., Fukuda, H.
  • Journal Title: Proc Nail Acad Sci USA 105 Pages: 15208-15213
• [Journal Article] Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis. (2008)
  • Author(s): Ohyama, K., Ogawa, M., Matsubayashi, Y.
  • Journal Title: Plant J. 55 Pages: 152-160
• [Journal Article] Tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis. (2007)
  • Author(s): Amano, Y., Tsubouchi, H., Shinohara, H., Ogawa, M., Matsubayashi, Y.
  • Journal Title: Proc Natl Acad Sci USA 104 Pages: 18333-18338
• [Journal Article] Identification of ligand binding site of phytosulfokine receptor by on-column photoaffinity labeling. (2007)
  • Author(s): Shinohara, H., Ogawa, M., Sakagami, Y., Matsubayashi, Y.
  • Journal Title: J Biol Chem 282 Pages: 124-131
• [Journal Article] Functional immobilization of plant receptor-like kinase onto microbeads towards receptor array construction and receptor-based ligand fishing. (2007)
  • Author(s): Shinohara, H., Matsubayashi, Y.
  • Journal Title: Plant J 52 Pages: 175-184
• [Presentation] Identification of a biologically active, small, secreted peptide in Arabidopsis by in silico gene screening, followed by LC-MS-based structure analysis. (2008)
  • Author(s): Matsubayashi, Y., Ohyama, K., Ogawa, M.
  • Organizer: Japan-Korea Symposium-Plant Growth and Signal Transduction.
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20080609-20080610
• [Presentation] Physiological functions of two structurally distinct tyrosine-sulfated peptides in Arabidopsis growth and development. (2007)
  • Author(s): Matsubayashi Y.
  • Organizer: 18th International Conference on Arabidopsis Research.
  • Place of Presentation: Beijing, China
  • Year and Date: 20070720-20070723
• [Book] ペプチペプチドリガンド-受容体ペアの解析から探る植物のかたちづくり. 蛋白質核酸酵素 (2009)
  • Author(s): 松林嘉克
  • Total Pages: 49-57
  • Publisher: 秀潤社
• [Book] リガンドー受容体ペアの解析から探る植物の細胞間シグナリング. 化学と生物 (2008)
  • Author(s): 松林嘉克, 篠原秀文
  • Total Pages: 588-590
  • Publisher: 学会出版センター
• [Remarks]
  • URL: http://www.agr.nagoya-u,ac.jp/~bioact/matsu/index.html