Site-specific mesenchymal-epithelial interactions elucidate pathogenesis of skindiseases and contribute to perfect tissue regeneration.

2008 Fiscal Year Final Research Report

• Project/Area Number: 18689028
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Nagoya City University (2007-2008); Osaka University (2006)
• Principal Investigator: YAMAGUCHI Yuji Nagoya City University, 大学院・医学研究科, 准教授 (00335340)
• Project Period (FY): 2006 – 2008
• Keywords: 皮膚腫瘍学 / 創傷治癒学 / 再生医学 / 色素細胞学 / 皮膚発生学

Research Abstract

「ヒト成人においても、上皮間葉系相互作用(真皮線維芽細胞がその上にある上皮系細胞に与える作用)により部位特異性(皮膚のさまざまな部位で性質が異なること)を決定付けていること」を実証してきた。手のひら・足のうらの皮膚は、分厚い皮膚で外からの刺激に強い・毛が無い・色素が薄いという3つの特徴がある。手のひら・足のうらの真皮線維芽細胞で発現が高いDKK1が、メラノサイトの機能を抑えるだけでなく、表皮細胞のメラニン取り込み能を抑制すると同時に、重層化を促進している結果を得ることが出来た。

Research Products

• [Journal Article] Regulation of skin pigmentation and thickness by DKK1 (2009)
  • Author(s): Yamaguchi Y(4名・筆頭・責任著者) Morita A, et al.
  • Journal Title: J Investig DermatolSymp Proc (in press)
  • Peer Reviewed
• [Journal Article] Intractable wounds caused by pyoderma gangrenosum in a patient with critical limb ischemia treated with cyclosporine and adjuvant sympathectomy. (2009)
  • Author(s): Takahashi Y, Yamaguchi Y(6名・共筆頭・責任著者), et al.
  • Journal Title: Dermatol Surg (Epub)
  • Peer Reviewed
• [Journal Article] Short- and long-term effects of UV radiation on the skin. (2009)
  • Author(s): Coelho SG, 中略, Yamaguchi Y(15名・5番目), et al.
  • Journal Title: J Investig Dermatol Symp Proc (in press)
  • Peer Reviewed
• [Journal Article] Molecular basis of premature skin aging induced by tobacco smoking. (2009)
  • Author(s): Morita A, 中略, Yamaguchi Y(4名・最終著者)
  • Journal Title: J Investig Dermatol Symp Proc (in press)
  • Peer Reviewed
• [Journal Article] Stage III melanoma treated with chemotherapy after surgery during the second trimester of pregnancy. (2009)
  • Author(s): Ishida I, Yamaguchi Y(7名・共筆頭・責任著者), T et al.
  • Journal Title: ArchDermatol 145(3) Pages: 346-8
  • Peer Reviewed
• [Journal Article] The development of a filter to enhance the efficacy and safety of excimer light (308 nm) therapy. (2009)
  • Author(s): Kobayashi K, 中略, Yamaguchi Y(11名・10番目), et al.
  • Journal Title: Photodermatol Photoimmunol Photomed 25(1) Pages: 30-6
  • Peer Reviewed
• [Journal Article] Feasibility and a ccuracy of anewly developed hand-held device with a flat-type fluorescent lamp for measuring the minimal erythema dose for narrow-band UVB. (2009)
  • Author(s): Morita A, 中略, Yamaguchi Y(8名・7番目), et al.
  • Journal Title: Photodermatol Photoimmunol Photomed 25(1) Pages: 41-4
  • Peer Reviewed
• [Journal Article] Embryonic hair follicle fate change by augmented beta-catenin signaling through Shh and Bmp signaling. (2009)
  • Author(s): Suzuki K, Yamaguchi Y(13名・2番目), et al.
  • Journal Title: Development 136(3) Pages: 367-72
  • Peer Reviewed
• [Journal Article] Establishment of suction blister roof grafting by injection of local anesthesia beneath the epidermis : less painful and more rapid formation of blisters. (2008)
  • Author(s): Hanafusa T, Yamaguchi Y(14名・共筆頭・責任著者), et al.
  • Journal Title: J Dermatol Sci 50(3) Pages: 243-7
  • Peer Reviewed
• [Journal Article] BeerJZ, Hearing VJ. Melanin mediated apoptosis of epidermal cells damaged by ultraviolet radiation: factors influencing the incidence of skin cancer. (2008)
  • Author(s): Yamaguchi Y(3名・筆頭・責任著者)
  • Journal Title: Arch DermatolRes (300 Suppl 1) Pages: S43-50
  • Peer Reviewed
• [Journal Article] DKK1 regulates skin pigmentation and thickness by affecting Wnt/・-catenin signaling in keratinocytes. (2008)
  • Author(s): Yamaguchi Y(11名・筆頭・責任著者),Passeron T, et al.
  • Journal Title: FASEB J 22(4) Pages: 1009-20
  • Peer Reviewed
• [Journal Article] Involvement of dynein and spectrin with early melanosome transport and melanosomal protein trafficking. (2008)
  • Author(s): Watabe H, 中略, Yamaguchi Y(10名・4番目), et al.
  • Journal Title: J Invest Dermatol 128(1) Pages: 162-74
  • Peer Reviewed
• [Journal Article] Dynamics of pigmentation induction by repeated UV exposures: dose, dose interval and UV spectrum dependence. (2008)
  • Author(s): Miller SA, 中略, Yamaguchi Y(7名・5番目), et al.
  • Journal Title: Br J Dermatol 159 Pages: 921-30
  • Peer Reviewed
• [Journal Article] Congenital peristernal dermal sinuses: a new entity? (2008)
  • Author(s): Kubo T, 中略, Yamaguchi Y(5名・3番目),et al.
  • Journal Title: Br J Dermatol 159 Pages: 763-5
  • Peer Reviewed
• [Journal Article] Cyclobutane pyrimidine dimer formation and p53 production in human skin. (2008)
  • Author(s): Yamaguchi Y(7名・筆頭著者), Coelho SG, et al.
  • Journal Title: Exp Dermatol 17(11) Pages: 916-24
  • Peer Reviewed
• [Journal Article] Apocrine adenocarcinoma originating from the Moll's gland of the eyelid: report of a case. (2008)
  • Author(s): Kanazawa S, 中略, Yamaguchi Y(7名・4番目), et al.
  • Journal Title: Eur J Plast Surg 31 Pages: 333-6
  • Peer Reviewed
• [Journal Article] Katayama I. Intractable wounds caused by arteriosclerosis obliterans with end-stage renal disease treated by aggressive debridement and epidermal grafting. (2007)
  • Author(s): Hanafusa T, Yamaguchi Y(3名・責任著者),
  • Journal Title: J Am Acad Dermatol 57(2) Pages: 322-6
  • Peer Reviewed
• [Journal Article] Intractable wounds caused by calcific uremic arteriolopathy treated with bisphosphonates. (2007)
  • Author(s): Hanafusa T, Yamaguchi Y(6名・責任著者),et al.
  • Journal Title: J Am Acad Dermatol 57(6) Pages: 1021-5
  • Peer Reviewed
• [Journal Article] IRegulation of human skin pigmentation and responses to UV radiation. (2007)
  • Author(s): Miyamura Y, 中略, Yamaguchi Y(14名・12番目), et al.
  • Journal Title: Pigment Cell Res 20(1) Pages: 2-13
  • Peer Reviewed
• [Journal Article] The effects of dickkopf 1 on gene expression and Wnt signaling by melanocytes: mechanisms underlying its suppression of melanocyte function and proliferation. (2007)
  • Author(s): Yamaguchi Y(7名・筆頭・責任著者),Passeron T, et al.
  • Journal Title: J Invest Dermatol 127(5) Pages: 1217-25
  • Peer Reviewed
• [Journal Article] Intractable wounds from a herpe ssimplex infection in an immunosuppressed patient with rheumatoid arthritis. (2007)
  • Author(s): Hanafusa T, Yamaguchi Y(4名・責任著者), et al.
  • Journal Title: Arch Dermatol 143(10) Pages: 1340-2
  • Peer Reviewed
• [Journal Article] Brenner M, Hearing VJ. The regulation of skin pigmentation. (2007)
  • Author(s): Yamaguchi Y(3名・筆頭著者),
  • Journal Title: J Biol Chem 282(38) Pages: 27557-61
  • Peer Reviewed
• [Journal Article] Sialylated core 1 O-glycans influence the sorting of Pmel17/gp100 and determine its capacity to form fibrils. (2007)
  • Author(s): Valencia JC, Yamaguchi Y(14名・6番目), et al.
  • Journal Title: J Biol Chem 282(15) Pages: 11266-80
  • Peer Reviewed
• [Journal Article] UV increases the nuclear localization of APE/Ref-1 in human skin. (2006)
  • Author(s): Takahashi K, 中略, Yamaguchi Y(7名・3番目), et al.
  • Journal Title: J Invest Dermatol 126(12) Pages: 2723-6
  • Peer Reviewed
• [Journal Article] Human skin responses to UV radiation: pigment in the upper epidermis protects against DNA damage in the lower epidermis and facilitates apoptosis. (2006)
  • Author(s): Yamaguchi Y(9名・筆頭著者), TakahashiK, et al.
  • Journal Title: FASEB J 20(9) Pages: 1486-8
  • Peer Reviewed
• [Journal Article] Sorting of Pmel17 to melanosomes through the plasma membrane by AP1 and AP2 : evidence for the polarized nature of melanocytes. (2006)
  • Author(s): Valencia JC, 中略, Yamaguchi Y(14名・8番目), et al.
  • Journal Title: J Cell Sci 119(Pt 6) Pages: 1080-91
  • Peer Reviewed
• [Journal Article] Suppression of tumorigenicity, but not anchorage independence, of human cancer cells by new candidate tumor suppressor gene CapG. (2006)
  • Author(s): Watari A, 中略, Yamaguchi Y(14名・8番目), et al.
  • Journal Title: Oncogene 25(56) Pages: 7373-80
  • Peer Reviewed
• [Journal Article] Regulation of constitutive and UVR-induced skin pigmentation by melanocortin 1 receptor isoforms. (2006)
  • Author(s): Rouzaud F, 中略, Yamaguchi Y(10名・3番目), et al.
  • Journal Title: FASEB J 20(11) Pages: 1927-9
  • Peer Reviewed
• [Presentation] Adult dermal fibroblasts determine the fate of the overlying epidermis. (2009)
  • Author(s): Yamaguchi Y.
  • Organizer: The Global COE Liaison Laboratory regular seminar
  • Place of Presentation: [Invited lecturer] Kumamoto, Japan.
  • Year and Date: 2009-05-04
• [Presentation] The effect of UV on human skin pigmentation. (2009)
  • Author(s): Yamaguchi Y.
  • Organizer: The 3rd Annual Symposium in Institute of Dermatological Science
  • Place of Presentation: [Invited lecturer] Seoul, Korea.
  • Year and Date: 2009-02-06
• [Presentation] Melanocyte Biology (2009)
  • Author(s): Yamaguchi Y .
  • Organizer: Pre-seminar for the 3rd Annual Symposium in Institute of Dermatological Science
  • Place of Presentation: [Invited lecturer] Seoul, Korea.
  • Year and Date: 2009-02-05
• [Presentation] Workshop : CS-14 Molecular and surgical pathology. (2008)
  • Author(s): Yamaguchi Y.
  • Organizer: The 20th International Pigment Cell Conference and 5th International Melanoma Research Conference.
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20080507-12
• [Presentation] Regulation of skin pigmentation and thickness by dickkopf 1(DKK1). (2008)
  • Author(s): Yamaguchi Y.
  • Organizer: IID 2008 Satellite Symposium Photoaging Research Symposium
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2008-05-13
• [Presentation] Epidermal sheet grafting for chronic wound.Workshop : Modern wound management. (2007)
  • Author(s): Yamaguchi Y.
  • Organizer: The 21st World Congress of Dermatology.
  • Place of Presentation: Buenos Aires, Argentina
  • Year and Date: 20070930-1005
• [Presentation] The effects of dickkopf 1 on gene expression and Wnt signaling by keratinocytes: mechanisms regulating skin pigmentation and thickness. (2007)
  • Author(s): Yamaguchi Y, Passeron T, Katayama I, Hearing VJ.
  • Organizer: The 68th Annual Meeting of the Society for Investigative Dermatology.
  • Place of Presentation: LosAngeles, CA, USA
  • Year and Date: 20070509-12
• [Presentation] Regulation of skin pigmentation. (2007)
  • Author(s): Yamaguchi Y .
  • Organizer: Avon Lecture Series for Pigment Cell Research.
  • Place of Presentation: New York, NY, USA
  • Year and Date: 2007-05-15
• [Book] Melanocyte distribution and function in human skin (2006)
  • Author(s): Yamaguchi Y(2名・筆頭著者)
  • Total Pages: 101-115
  • Publisher: Humana Press