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2008 Fiscal Year Final Research Report

Functional analysis of a novel Nucleolar protein complex.

Research Project

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Project/Area Number 18689902
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionUniversity of Tsukuba

Principal Investigator

MURAYAMA Akiko  University of Tsukuba, 大学院・生命環境科学研究科, 講師 (50431656)

Project Period (FY) 2006 – 2008
Keywords核小体 / がん治療 / クロマチン / DNAメチル化酵素 / rRNA
Research Abstract

Nucleomethylin(NML)という新規核小体蛋白質が細胞培養液中のグルコースを減らすと、ヒト培養細胞中のリボソーム合成やたんぱく質産生量が低下することを見出した。NMLは核小体に存在し、NAD依存的脱アセチル化酵素SIRT1やヒストンH3K9メチル化酵素SUV39H1と複合体を形成する。NML複合体はrDNA領域をヘテロクロマチン化することによって栄養飢餓状態でのrRNA合成抑制に働くことを明らかにした。また、NML複合体の機能破綻はエネルギーバランスの崩壊と、それに伴う細胞死を引き起こすことから、栄養飢餓による細胞死の回避に働いていることも判明した。

  • Research Products

    (17 results)

All 2010 2009 2008 2007 2006 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] Estrogen inhibits TGF-{beta} signaling by promoting Smad2/3 degradation.2010

    • Author(s)
      Ito I, Hanyu A, Wayama M, Goto N, Katsuno Y, Kawasaki S, Nakajima Y, Kajiro M, Komatsu Y, Fujimura A, Hirota R, Murayama A, Kimura K, Imamura T, Yanagisawa J
    • Journal Title

      The Journal of biological chemistry (In press)

    • Peer Reviewed
  • [Journal Article] The chromosomal association of condensin II is regulated by a noncatalytic function of PP2A.2009

    • Author(s)
      Takemoto A, Maeshima K, Ikehara T, Yamaguchi K, Murayama A, Imamura S, Imamoto N, Yokoyama S, Hirano T, Watanabe Y, Hanaoka F, Yanagisawa J, Kimura K
    • Journal Title

      Nature Structural & Molecular Biology Vol.16,No.12

      Pages: 1302-1308

    • Peer Reviewed
  • [Journal Article] The nucleolar protein NML regulates hepatic ATP levels during liver regeneration after partial hepatectomy.2009

    • Author(s)
      Mikogai A, Yanagisawa J, Yasuzawa-Tanaka K, Murayama A
    • Journal Title

      Biochemical and Biophysical Research Communications Vol.390,No3

      Pages: 591-596

    • Peer Reviewed
  • [Journal Article] KLF4 suppresses estrogen-dependent breast cancer growth by inhibiting the transcriptional activity of ERα.2009

    • Author(s)
      Akaogi K, Nakajima Y, Ito I, Kawasaki S, Oie S, Murayama A, Kimura K, Yanagisawa J
    • Journal Title

      Oncogene Vol.28,No32

      Pages: 2894-2902

    • Peer Reviewed
  • [Journal Article] The ubiquitin ligase CHIP acts as an upstream regulator of oncogenic pathways.2009

    • Author(s)
      Kajiro M, Hirota R, Nakajima Y, Kawanowa K, So-ma K, Ito I, Yamaguchi Y, Ohie S, Kobayashi Y, Seino Y, Kawano M, Kawabe YI, Takei H, Hayashi S, Kurosumi M, Murayama A, Kimura K, Yanagisawa J
    • Journal Title

      Nature Cell Biol. Vol.11,No3

      Pages: 312-319

    • Peer Reviewed
  • [Journal Article] Epigenetic control of rDNA loci in response to intracellular energy status.2008

    • Author(s)
      Murayama A, Ohmori K, Fujimura A, Minami H, Yasuzawa-Tanaka K, Kuroda T, Oie S, Daitoku H, Okuwaki M, Nagata K, Fukamizu A, Kimura K, Simizu T, Yanagisawa J
    • Journal Title

      Cell. Vol.133

      Pages: 627-639

    • Peer Reviewed
  • [Journal Article] PPARγligands suppress the feedback loop between E2F2 and cyclin-E1.2008

    • Author(s)
      Komatsu Y, Ito I, Wayama M, Fujimura A, Akaogi K, Machida H, Nakajima Y, Kuroda T, Ohmori K, Murayama A, Kimura K, Yanagisawa J
    • Journal Title

      Biochem Biophys Res Commun. Vol.370,No.1

      Pages: 145-148

    • Peer Reviewed
  • [Journal Article] Analysis of the role of Aurora B on the chromosomal targeting of condensing I.2007

    • Author(s)
      Takemoto A, Murayama A, Katano M, Urano T, Furukawa K, Yokoyama S, Yanagisawa J, Hanaoka F, Kimura K
    • Journal Title

      Nucleic Acids Res. Vol.35

      Pages: 2403-2412

    • Peer Reviewed
  • [Journal Article] Turning Off Estrogen Receptor β-Mediated Transcription Requires Estrogen-Dependent Receptor Proteolysis.2006

    • Author(s)
      Tateishi Y, Sonoo R, Sekiya Y, Sunahara N, Kawano M, Wayama M, Hirota R, Kawabe YMurayama A, Kato S, Kimura K, Yanagisawa J
    • Journal Title

      Mol. Cell. Biol. Vol.26

      Pages: 7966-7976

    • Peer Reviewed
  • [Journal Article] A specific CpG site demethylation in the human interleukin 2 gene promoter is an epigenetic memory.2006

    • Author(s)
      Murayama A, Sakura K, Nakama M, Yasuzawa-Tanaka K, Fujita E, Tateishi Y, Wang Y, Ushijima T, Shibuya K, Kawabe Y, Yanagisawa J
    • Journal Title

      EMBO J. Vol.25

      Pages: 1081-1092

    • Peer Reviewed
  • [Presentation] 核小体因子Nucleomethylinによるリボソーム合成調節機構と細胞内エネルギー代謝2010

    • Author(s)
      村山明子
    • Organizer
      2010年度日本農芸化学会シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2010-03-30
  • [Presentation] エピジェネティック機構を介した細胞内エネルギー代謝制御2009

    • Author(s)
      村山明子
    • Organizer
      日本レチノイド研究会第20回学術集会・ランチョンセミナー
    • Place of Presentation
      東京
    • Year and Date
      2009-11-21
  • [Presentation] 核小体因子Nucleomethylinによる核内反応制御機構の解析2009

    • Author(s)
      村山明子
    • Organizer
      第82回日本生化学会大会シンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-23
  • [Presentation] rDNA領域のエピジェネティックな制御による細胞内エネルギー調節機構2008

    • Author(s)
      村山明子
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会・シンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-11
  • [Presentation] a novel protein complex, senses intracellular energy status and epigenetically controls the rDNA locus.2008

    • Author(s)
      Akiko Murayama, Kazuji Ohmori, Akiko Fujimura, Kayoko Yasuzawa-Tanaka, Shohei Oie, Toshiyuki Shimizu, Junn Yanagisawa eNoSC
    • Organizer
      第21回内藤コンファレンス
    • Place of Presentation
      八ヶ岳
    • Year and Date
      2008-06-26
  • [Presentation] a novel protein complex2008

    • Author(s)
      Akiko Murayama, Kazuji Ohmori, Akiko Fujimura, Kayoko Yasuzawa-Tanaka, Takao Kuroda, Shohei Oie, Toshiyuki Shimizu, Junn Yanagisawa eNoSC
    • Organizer
      epigenetically controls the rDNA locus Metabolic Pathways of Longevity・Keystone Symposium
    • Place of Presentation
      Denver
    • Year and Date
      2008-04-01
  • [Remarks]

    • URL

      http://yanagisawalab.org/

URL: 

Published: 2011-06-18   Modified: 2016-04-21  

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