Study of cryopreservation using vitrification

2008 Fiscal Year Final Research Report

• Project/Area Number: 18710203
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Resource conservation science
• Research Institution: Akita Prefectural University (2008); National Institute of Agrobiological Sciences (2006-2007)
• Principal Investigator: TANAKA Daisuke Akita Prefectural University, 生物資源科学部, 流動研究員 (60425593)
• Project Period (FY): 2006 – 2008
• Keywords: ガラス化 / 資源保全学 / 絶滅危惧種 / 生体膜 / 水 / アクアポリン / 生物多様性保全 / 細胞内微細構造

Research Abstract

植物細胞・組織の長期保存におけるガラス化と生存性との関係を明らかにした.組織細胞の液体窒素保存時の応答および水の挙動,熱分析などの基礎的な知見を得て,論理的かつ成功的な植物遺伝資源保存を可能にする手法'ドロップレット'法を開発した.この方法により普及可能な操作で多様な絶滅危惧植物遺伝資源を高い生存率で保存することが可能になった.

Research Products

• [Journal Article] Role of the aquaporin PIP1 subfamily in the chilling tolerance of rice (2009)
  • Author(s): T. Matusumoto, H. L. Lian, W. A. Su, D. Tanaka, C. W. Liu, I. Iwasaki, Y. Kitagawa
  • Journal Title: Plant and cell Physiology 50(2) Pages: 216-229
  • Peer Reviewed
• [Journal Article] 岩手県北東部の雑穀遺伝資源の探索 (2008)
  • Author(s): 小館琢磨,田中大介
  • Journal Title: 植物遺伝資源探索導入調査報告書 Vol.24 Pages: 33〜39
  • Peer Reviewed
• [Journal Article] 農業生物資源ジーンバンク事業における超低温保存の利用 (2008)
  • Author(s): 白田和人,福井邦明,田中大介,新野孝男
  • Journal Title: 低温生物工学会誌 54 Pages: 9-14
  • Peer Reviewed
• [Journal Article] ガラス化による植物茎頂の保存 (2008)
  • Author(s): D. Tanaka
  • Journal Title: NSL News Letter 2007-2 Pages: 24〜27
• [Journal Article] Cryopreservation of basal stem buds of in vitro-grown matrush (Juncus SPP.) by virtification (2008)
  • Author(s): T. Niino, D. Tanaka, R. R. Tantely, K. Fukui K. Shirata
  • Journal Title: CryoLetters Vol.28(3) Pages: 197-206
  • Peer Reviewed
• [Journal Article] Cryopreservation of shoottips of endangered Hayachine-usuyukiso (Leontopodium hayachinense (Takeda) Hara et Kitam) using vitrification protocol (2008)
  • Author(s): D. Tanaka, T. Niino, Y. Tsuchiya, M. Uemura
  • Journal Title: Plant Genetic Resouces 6 Pages: 164-166
  • Peer Reviewed
• [Journal Article] Cryopreservation of in vitro Grown Black Chokeberry shoot tips by Droplet vitrification
  • Author(s): D. Tanaka, T. Matsumoto, A. Nishiuchi, T. Niino
  • Journal Title: Acta Horticulturae (In press)
  • Peer Reviewed
• [Presentation] What Differences Are There Between Droplet Vitrification and Vitrification Protocols of Cryopreservation? (2009)
  • Author(s): D. TANAKA, T. Matsumoto, A, Nishiuchi, T. Niino
  • Organizer: The first International Symposium on Cryopreservation in Horticultural Species
  • Place of Presentation: Leuven, Belgium
  • Year and Date: 20090300
• [Presentation] Ultrastructural evidence for the effects of rapid cooling on plant shoot apices by the vitrification based protocol using cryo-scanning electron microscopy (2009)
  • Author(s): D. TANAKA, T. Niino, T. Matsumoto, S. Fujikawa
  • Organizer: The first International Symposium on Cryopreservation in Horticultural Species
  • Place of Presentation: Leuven, Belgium
  • Year and Date: 20090300
• [Presentation] イネ低温耐性におけるOsPIP1 ; 3の役割 (2009)
  • Author(s): T. Matusumoto, H. L. Lian, W. A. Su, D. Tanaka, C. W. Liu, I. Iwasaki, Y. Kitagawa
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学, 愛知県
  • Year and Date: 20090300
• [Presentation] 麹菌(Aspergillus Oryzae)におけるアクアポリンの機能解明 (2008)
  • Author(s): 田中大介,松本直,岩崎郁子,北川良親
  • Organizer: 第8回糸状菌分子生物学コンファレンス
  • Place of Presentation: 石川県文京会館
  • Year and Date: 20081100
• [Presentation] イグサ遺伝資源の超低温保存 (2007)
  • Author(s): 田中大介,新野孝男,R. R. Tantely,福井邦明,白田和人
  • Organizer: 園芸学会
  • Place of Presentation: 高知大学
  • Year and Date: 20071000
• [Presentation] Ultrastructural observation of plasma membrane of plant shoot apices in vitrification-based protocol (2007)
  • Author(s): D. Tanaka, T. Niino, M. Uemura S. Fujikawa
  • Organizer: CRYO2007(44th MEETING OF THE SOCIETY FOR CRYOBIOLOGY)
  • Place of Presentation: Lake Louise, Canada
  • Year and Date: 20070700
• [Presentation] ガラス化長期保存法における原形質膜超微細構造の観察 (2007)
  • Author(s): 田中大介,行弘文子,西内明子,新野孝男
  • Organizer: 園芸学会
  • Place of Presentation: 京都テレサ
  • Year and Date: 20070300
• [Presentation] ガラス化法における冷却時の細胞挙動と新たな保存法への試み (2006)
  • Author(s): 田中大介
  • Organizer: 日本植物細胞分子生物学会
  • Place of Presentation: 筑波大学
  • Year and Date: 20060700
• [Presentation] ドロップレット法を用いたアロニア培養茎頂の超低温保存法開発 (2006)
  • Author(s): 田中大介,西内明子,新野孝男
  • Organizer: 園芸学会
  • Place of Presentation: 千葉大学
  • Year and Date: 20060300
• [Book] Reed B. M. edited, Springer verlag (2008)
  • Author(s): D. Tanaka
  • Total Pages: 4
  • Publisher: Plant Cryopreservation