Long chain DNA folding by nanoparticles: Construction of Artificial Chromatin Model

2008 Fiscal Year Final Research Report

• Project/Area Number: 18719001
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Nagoya University
• Principal Investigator: ZINCHENKO A.a. Nagoya University, 大学院・環境学研究科, 講師 (00432352)
• Project Period (FY): 2006 – 2008
• Keywords: DNA / ナノ粒子 / クロマチン / ナノ構造 / 転写活性 / 生体模倣モデル / 自己組織化 / 単一分子観察

Research Abstract

細胞内のゲノムDNAはヒストン蛋白質との複合体を形成し、高次構造へ階層的に折り畳まれている。このDNAとヒストンの複合体状態が変化することでDNA遺伝子活性が自然制御されている。本研究では、DNAとヒストンサイズのナノ粒子からなる「人工クロマチン」という単純なモデルを化学的構築し、DNAとナノ粒子との相互作用および自己組織化の制御へアプローチを開発した。そこで、人工クロマチン高次構造と人工クロマチン中のDNAの生物学的活性の関係を明らかにした。人工クロマチン系の展開により、将来の遺伝子治療の新技術としてDNAバイオ活性を化学的制御が可能になると期待されている。

Research Products

• [Journal Article] A Novel Type Cationic Silica Nanoparticles and its Potential Application in Biomedicine (2008)
  • Author(s): Li L., Takenaka T., Zinchenko A., Ning C, Yue M, Yoshikawa, K.
  • Journal Title: International Conference on Bioinformatics and Biomedical Engineering 2008, ICBBE 2008, 16-18 May, 2008 Pages: 1539-1542
  • Peer Reviewed
• [Journal Article] Conformational Behavior of Giant DNA through Binding with Ag+ and Metallization (2008)
  • Author(s): Zinchenko A. A., Baigl D, Chen N., Pyshkina O. A., Endo K., Sergeyev V. G., Yoshikawa K.
  • Journal Title: Biomacromolecules 9 Pages: 1981-1987
  • Peer Reviewed
• [Journal Article] Single-Chain Compaction of Long Duplex DNA by Cationic Nanoparticles: Modes of Interaction and Comparison with Chromatin (2007)
  • Author(s): Zinchenko A. A., Sakaue T., Araki S., Yoshikawa K., Baigl D.
  • Journal Title: J. Phys. Chem. B 111 Pages: 3019-3031
  • Peer Reviewed
• [Journal Article] Transcription of Giant DNA Complexed with Cationic Nanoparticles as a Simple Model of Chromatin (2007)
  • Author(s): Zinchenko A. A., Luckel F., Yoshikawa K.
  • Journal Title: Biophys. J. 92 Pages: 1318-1325
  • Peer Reviewed
• [Journal Article] Visualization of Different Pathways of DNA Release from Interpolyelectrolyte Complex (2007)
  • Author(s): Ayoubi M. A., Zinchenko A. A., Philippova O. E, Khokhlov A. R., Yoshikawa K.
  • Journal Title: J. Phys. Chem. B 111 Pages: 8378-8378
  • Peer Reviewed
• [Journal Article] Cationic Silica Nanoparticles are Efficiently Transferred into Mammalian Cells (2007)
  • Author(s): Liu L, Takenaka T., Zinchenko A.A., Chen N., Inagaki S., Asada H., Kishida T., Mazda O, Murata S., Yoshikawa K.
  • Journal Title: 2007 International Symposium on Micro-Nano Mechatronics and Human Science, Nagoya, Japan, 11-14 November, 2007 Pages: 281-285
  • Peer Reviewed
• [Journal Article] Dynamic Conformational Behavior and Molecular Interaction Discrimination of DNA/Binder Complexes by Single-Chain Stretching in a Micro Device (2007)
  • Author(s): Huang W.-H., Zinchenko A. A., Pawlak C., Chen Y., Baigl D.
  • Journal Title: Chem Bio Chem 8(15) Pages: 1771-1774
  • Peer Reviewed
• [Journal Article] Na+ more strongly inhibits DNA compaction by spermidine (3+) than K+ (2006)
  • Author(s): Hibino K., Yoshikawa Y., Murata S., Saito T., Zinchenko A. A., Yoshikawa K.
  • Journal Title: Chem. Phys. Lett. 426 Pages: 405-409
  • Peer Reviewed
• [Presentation] 簡単なクロマチンモデルとしてDNA-カチオン性ナノ粒子複合体の形成 (2007)
  • Author(s): ジンチェンコ アナトーリ
  • Organizer: 第38回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 三重
  • Year and Date: 20071100
• [Book] Single DNA molecules: Compaction and decompaction. in DNA Interactions with Polymers and Surfactants(ed. Lindman B., Dias R.) (2008)
  • Author(s): Zinchenko A. A., Pyshkina O. A., Lezov A. V., Sergeyev V. G., Yoshikawa K.
  • Total Pages: 59-88
  • Publisher: Willey-VCH
• [Book] Nanostructures and organizations of compacted single chains of polyelectrolytes, (Chapter 17) in Polymeric nanostructures and their applications(Ed. Nalwa H. S.) (2007)
  • Author(s): Zinchenko A. A., Baigl D., Yoshikawa K.
  • Publisher: American Scientific Publishers