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2008 Fiscal Year Final Research Report

Ion transporting mechanism of V-type Na+-ATPase from Enterococcus hirae

Research Project

  • PDF
Project/Area Number 18770122
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionKyoto University (2007-2008)
The Institute of Physical and Chemical Research (2006)

Principal Investigator

MURATA Takeshi  Kyoto University, 大学院医学研究科, 助教 (80415322)

Project Period (FY) 2006 – 2008
KeywordsV-ATPase / X線結晶構造解析 / ポンプ / ナトリウム / モーター / 複合体 / 膜タンパク質 / Enterococcus hirae
Research Abstract

研究代表者らは腸内連鎖球菌に存在するNa+輸送性V 型ATPase の分子生物学的・生化学的・構造生物学的研究を展開し、膜タンパク質部分であるNtpKリングのX線結晶構造解析に成功していた。本研究では本酵素のイオン輸送機構の解明を目的に、Li+結合型及び阻害剤DCCD結合型のNtpKリングのX線結晶構造を明らかにした。これらの構造と生化学的解析から、本酵素のイオン結合/解離のメカニズムが明らかになった。

  • Research Products

    (13 results)

All 2008 2007 2006

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (7 results) Book (2 results)

  • [Journal Article] Ion binding and selectivity of the rotor ring of the Na+-transporting V-ATPase2008

    • Author(s)
      Takeshi Murata, Ichiro Yamato, Yoshimi Kakinuma, Mikako Shirouzu, John E. Walker, Shigeyuki Yokoyama, and So Iwata
    • Journal Title

      Proc. Natl. Acad. Sci. U. S. A 105

      Pages: 8607-8612

    • Peer Reviewed
  • [Journal Article] Interaction and Stoichiometry of the Peripheral Stalk Subunits NtpE and NtpF and the N-terminal hydrophilic domain of NtpI, of Enterococcus hirae V-ATPase2008

    • Author(s)
      Misaki Yamamoto, Satoru Unzai, Shinya Saijo, Kazuki Ito, Kenji Mizutani, Chiyo Suno-Ikeda, Yukako Yabuki-Miyata, Takaho Terada, Mitsutoshi Toyama, Mikako Shirouzu, Takuya Kobayashi, Yoshimi Kakinuma, Ichiro Yamato, Shigeyuki Yokoyama, So Iwata, and Takeshi Murata
    • Journal Title

      J. Biol. Chem 283

      Pages: 19422-19431

    • Peer Reviewed
  • [Journal Article] E stimation of ligand binding free energies of F-ATPase by using molecular dynamics/free energy calculation2008

    • Author(s)
      Atsushi Suenaga, Osamu Umezu, Tadashi Ando, Ichiro Yamato, Takeshi Murata, Makoto Taiji
    • Journal Title

      J. Comput. Chem. Jpn 7

      Pages: 103-116

    • Peer Reviewed
  • [Journal Article] Deletion analysis of the subunit genes of V-type Na+-ATPase from Enterococcus hirae2006

    • Author(s)
      Toshiaki Hosaka, Kazuma Takase, Takeshi Murata, Yoshimi Kakinuma, and Ichiro Yamato
    • Journal Title

      Journal of Biochemistry 139

      Pages: 1045-1052

    • Peer Reviewed
  • [Presentation] Engineering of the rotor ring of the Na+ motive V-ATPase from Enterococcus hirae to examine the stoichiometry2008

    • Author(s)
      水谷健二
    • Organizer
      第46回日本生物物理学会
    • Place of Presentation
      福岡
    • Year and Date
      2008-12-03
  • [Presentation] V型ATPaseのイオン透過機構を考える2007

    • Author(s)
      村田武士
    • Organizer
      第45回日本生物物理学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-23
  • [Presentation] 液胞型イオン輸送ATPアーゼの分子制御とメカニズム2007

    • Author(s)
      村田武士、山登一郎、柿沼喜己
    • Organizer
      第30回日本分子生物学会・第80回日本生化学会(合同大会)
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-15
  • [Presentation] ION BINDING AND SELECTIVITY OF THE ROTOR OF THE V-TYPE NA+-ATPASE FROM ENTEROCOCCUS HIRAE2007

    • Author(s)
      Takeshi Murata
    • Organizer
      Asian Crystallographic Association 2007
    • Place of Presentation
      台湾(台北)
    • Year and Date
      2007-11-06
  • [Presentation] Ion transport mechanism of V-ATPase2007

    • Author(s)
      Takeshi Murata
    • Organizer
      The SOKENDAI International Symposium (37th SERIKEN International Symposium ; 2nd Symposium on the Partnership Project on Frontiers of Membrane Protein Research ; Satellite Symposium of the 84th Annual Meeting of the Physiological Society of JAPAN)
    • Place of Presentation
      Okazaki (Japan)
    • Year and Date
      2007-03-15
  • [Presentation] Structure of Li+ bound rotor ring of the V-type Na+-ATPase from Enterococcus hirae2006

    • Author(s)
      Takeshi Murata
    • Organizer
      Gordon Research Conference in Molecular and Cellular Bioenergetics
    • Place of Presentation
      New Hampshire( アメリカ)
    • Year and Date
      20060600
  • [Presentation] V型AT Paseの構造と機能2006

    • Author(s)
      村田武士
    • Organizer
      第6回蛋白質科学会
    • Place of Presentation
      京都
    • Year and Date
      2006-04-26
  • [Book] 蛋白質核酸酵素 Vol. 52 No. 4, ,(V-ATPase の新しい機能とその構造からみえてきたメカニズム)2007

    • Author(s)
      村田武士、山登一郎、柿沼喜己、横山謙
    • Total Pages
      335 -341
  • [Book] 生物物理、Vol.46, No. 6, (V型Na+-ATPaseの膜内在性ローターリングの構造 : 結晶構造から見えてきた回転モーターのイオン透過機構)2006

    • Author(s)
      村田武士、山登一郎、柿沼喜己
    • Total Pages
      336-340

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Published: 2010-06-10   Modified: 2016-04-21  

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