骨分化シグナル分子としての骨シアロタンパク質の機能の解明

2007 Fiscal Year Annual Research Report

• Project/Area Number: 18770196
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: 早田 匡芳 Tokyo Medical and Dental University, 難治疾患研究所, 助教 (40420252)
• Keywords: BSP / ノックアウトマウス / 骨形成 / マイクロアレイ

Research Abstract

骨シアロタンパク質(BSP)シグナルによってその発現が支配される遺伝子を同定するために、BSPノックアウトマウスと野生型マウス(生後0日齢)の頭蓋骨より、RNAを抽出し、Affymetrix GeneChipによるマイクロアレイ解析を行った。データ解析の結果、5倍以上発現増加した遺伝子は80個、発現減少した遺伝子は75個あった。遺伝子の生物学的特徴をGene ontologyに従って分類した結果、発現増加遺伝子グループのトップ3のカテゴリーは、transcription (9.3%), cell adhesion (6.7%), transport (5.3%)だった。なお、分類不明は45.3%だった。発現減少遺伝子グループのトップ3カテゴリーは、transcription (12.5%), transport (8.8%), signal transduction (5%)だった。なお、分類不明は、52.5%だった。
これらの遺伝子グループのうち、骨代謝に関与する遺伝子を検索した結果、増加遺伝子グループでは、Dcn, Hifla, Odam, Vhlが含まれ、減少遺伝子グループには、Col10Oa1が含まれた。
今後は、これらの遺伝子群に着目し、発現の確認、BSPシグナルとの関連性を調べる計画である。

Research Products

• [Journal Article] Transient Receptor Potential Vanilloid 4 Deficiency Suppresses Un loading-Induced Bone Loss (2008)
  • Author(s): Mizoguchl F, Mizuno A, Hayata T, Nakashima K, Heller S, Ushida T, Sokabe M, Miyasaka N, Suzuki M, Ezura Y, Noda M
  • Journal Title: J Cell Physiol (In press)
  • Peer Reviewed
• [Journal Article] JunD suppresses bone formation and contributes to low bone mass induced by estrogen depletion (2008)
  • Author(s): Kawamata A, Izu Y, Yokoyama H, Amagasa T, Wagner EF, Nakashima K, Ezura Y, Hayata T, Noda M
  • Journal Title: J Cell Biochem 103 Pages: 1037-45
  • Peer Reviewed
• [Journal Article] Ciz, a transcription factor with a nucleocytoplasmic shuttling activity, interacts with C-propeptides of type I collagen (2008)
  • Author(s): Hayata T, Nakamoto T, Ezura Y, Noda M.
  • Journal Title: Biochem Biophys Res Commun 368 Pages: 205-10
  • Peer Reviewed
• [Journal Article] OPN deficiency suppresses appearance of odontoclastic cells and resorption of the tooth root induced by experimental force application (2008)
  • Author(s): Chung CJ, Soma K, Rittling SR, Denhardt DT, Hayata T, Nakashima K, Ezura Y, Noda M
  • Journal Title: J Cell Physiol 214 Pages: 614-20
  • Peer Reviewed
• [Journal Article] Xenopus cDNA microarray identification of genes with endodermal organ expression (2008)
  • Author(s): Park EC, Hayata T, Cho KW, Han JK
  • Journal Title: Dev Dyn 236 Pages: 183-90
  • Peer Reviewed
• [Journal Article] TGF-beta signaling-mediated morphogenesis: modulation of cell adhesion via cadherin endocytosis (2007)
  • Author(s): Ogata S, Morokuma J, Hayata T, Kolle G, Niehrs C, Ueno N, Cho, KW
  • Journal Title: Genes Dev 21 Pages: 1817-31
  • Peer Reviewed
• [Journal Article] Ciz, a transcription factor with a nucleocytoplasmic shuttling activity, interacts with C-propeptides of type I collagen (2007)
  • Author(s): Ono N, Nakashima K, Schipani E, Hayata T, Ezura Y, Soma K, Kronenberg HM, Noda M
  • Journal Title: J Biol Chem 282 Pages: 25509-16
  • Peer Reviewed
• [Journal Article] Xenopus Glucose transporter 1(xGLUT1)is required for gastrulation movement in Xenopus laevis (2007)
  • Author(s): Suzawa K, Yukita A, Hayata T, Goto T, Danno H, Michiue T, Cho K W, Asashima M
  • Journal Title: Int J Dev Biol 51 Pages: 183-90
  • Peer Reviewed
• [Journal Article] Serum osteopontin, an enhancer of tumor metastasis to bone, promotes B16 melanoma cell migration (2007)
  • Author(s): Hayashi C, Rittling S, Hayata T, Amagasa T, Denhardt D, Ezura Y, Nakashima K, Noda M
  • Journal Title: J Cell Biochem 101 Pages: 979-86
  • Peer Reviewed
• [Journal Article] The Role of XBtg2 in Xenopus Neural Development (2007)
  • Author(s): Sugimoto K, Okabayashi K, Sedohara A, Hayata T, Asashima M
  • Journal Title: Dev Neurosci 29 Pages: 468-79
  • Peer Reviewed
• [Presentation] β-adrenergic Signaling Requires Bone Matrix Protein OPN to Suppress Bone Formation and to Activate Bone Resorption (2008)
  • Author(s): M. Nagao, Y. Saita, J. Nagata, Y. Izu, T. Hayata, H. Hemmi, Y. Ezura, K. Nakashima, H. Kurosawa, M. Noda
  • Organizer: American Society for Bone Mineral and Research
  • Place of Presentation: Honoluu, Hawaii, USA
  • Year and Date: 20080916-19
• [Presentation] Angiotensin II suppresses bone mass via AT1 receptor in Long Bones of Adult Mice (2008)
  • Author(s): Y. Izu, T. Hayata, H. Hemmi, J. Nagata, Y. Ezura, K. Nakashima, M. Noda
  • Organizer: American Society for Bone Mineral and Research
  • Place of Presentation: Honoluu, Hawaii, USA
  • Year and Date: 20080916-19
• [Presentation] Responses of Bone in ANA Knock-out Mice to Ovariectomy (2008)
  • Author(s): K. Miyai, M. Yoneda, F. Mizoguchi, T. Hayata, Y. Ezura, K. Nakashima, T. Yamamoto, M. Noda
  • Organizer: American Society for Bone Mineral and Research
  • Place of Presentation: Honoluu, Hawaii, USA
  • Year and Date: 20080916-19
• [Presentation] Osteoclast Specific Ablation of Dicer Suppresses Bone Resorption and Increases Bone Mass (2008)
  • Author(s): F. Mizoguchi, T. Nakamura, T. Hayata, H. Hemmi, Y. Ezura, K. Nakashima, N. Miyasaka, B. Harfe, S. Kato, M. Noda
  • Organizer: American Society for Bone Mineral and Research
  • Place of Presentation: Honoluu, Hawaii, USA
  • Year and Date: 20080916-19
• [Presentation] Ciz, a Nucleo-cytoplasmic Shuttling Protein Interacts with Extracellular Matrix Proteins (2008)
  • Author(s): T. Hayata, T. Nakamoto, Y. Ezura, H. Hirai, M. Noda
  • Organizer: American Society for Bone Mineral and Research
  • Place of Presentation: Honoluu, Hawaii, USA
  • Year and Date: 2008-09-19
• [Presentation] β2 Adrenergic Receptor Deficiency Enhances Bone Mass in by Antagonizing Against Aging-induced Bone Loss and Blunts Anabolic Effects of PTH on Osteoblasts (2008)
  • Author(s): R. Hanyu, Y. Saita, J. Nagata, Y. Izu, T. Hayata, H. Hemmi, S. Takeda, Y. Ezura, K. Nakashima, H. Kurosawa, M. Noda
  • Organizer: American Society for Bone Mineral and Research
  • Place of Presentation: Honoluu, Hawaii, USA
  • Year and Date: 2008-09-16
• [Presentation] 転写因子CIZの欠損はK/BxN血清移入関節炎を抑制する (2007)
  • Author(s): 中元哲也、溝口史高、伊豆弥生、早田匡芳、江面陽一、宮坂信之、野田政樹
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 20071211-15
• [Presentation] Deficiency of CIZ suppresses serum-induced arthritis (2007)
  • Author(s): T. Nakamoto, F. Mizoguchi, Y. Izu, T. Hayata, Y. Ezura, N. Miyasaka, M. Noda
  • Organizer: American Society for Cell Biology
  • Place of Presentation: Washington D.C., USA
  • Year and Date: 20071201-05
• [Book] Principles of Developmental Genetics (2007)
  • Author(s): Hayata T and Cho KW
  • Total Pages: 32-44(1055)
  • Publisher: Elsevier/Academic Press