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2008 Fiscal Year Final Research Report

Intracellular kinetic analysis of oxygenases in whole-cell catalysts

Research Project

  • PDF
Project/Area Number 18780056
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionOsaka University

Principal Investigator

HONDA Kousuke  Osaka University, 大学院工学研究科, 助教 (90403162)

Project Period (FY) 2006 – 2008
Keywords生体機能利用 / 応用微生物 / 酵素反応 / 反応場制御
Research Abstract

酸化酵素反応において宿主微生物由来の因子が目的酵素の反応速度に及ぼす影響を明らかにすることを目的に、大腸菌1遺伝子破壊株を宿主とした網羅的スクリーニングを実施し、欠損により、酸化酵素反応速度を増長しうる3種類の遺伝子を同定した。

  • Research Products

    (3 results)

All 2008 2007

All Presentation (3 results)

  • [Presentation] Screening of Escherichia coli single-gene knockout mutant which could enhance a P450 activity2008

    • Author(s)
      Zhou, Y., Minami, T., Nishino, T., Nugroho, D.A., Honda, K., Omasa, T., Ohtake, H
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋
    • Year and Date
      20080300
  • [Presentation] Screening of Escherichia coli single-gene knockout mutants which are able to enhance the deethylation of 7-ethoxycoumarin catalyzed by CYP154A12007

    • Author(s)
      Zhou, Y., Honda, K., Omasa, T., Ohtake, H
    • Organizer
      BioMicroWorld2007
    • Place of Presentation
      Seville, Spain
    • Year and Date
      20071200
  • [Presentation] シトクロムP450モノオキシゲナーゼ活性に影響を及ぼす細胞構成因子の探索2007

    • Author(s)
      西野琢磨, Zhou Ying, Darmawan Ari Nugroho, 本田孝祐, 大政健史, 大竹久夫
    • Organizer
      生物工学会2007年度大会
    • Place of Presentation
      広島
    • Year and Date
      20070600

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Published: 2010-06-10   Modified: 2016-04-21  

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