Development of novel monitoring method of Paralytic shellfish poisoning

2008 Fiscal Year Final Research Report

• Project/Area Number: 18780146
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General fisheries
• Research Institution: Kobe University
• Principal Investigator: HOSOI Shoko (田辺 祥子) Kobe University, 内海域環境教育研究センター, 助教 (80423226)
• Project Period (FY): 2006 – 2008
• Keywords: 分子生物学 / 遺伝マーカー / バラスト水 / 移入種 / 麻痺生貝毒

Research Abstract

神経毒生産型渦鞭毛藻による麻痺性貝毒の被害を未然に防ぐため,高感度発生予察法を開発することを目的とし研究を行った。
手法としては,これまでの客観的で正確性に欠ける顕微鏡観察法に変わる手法として,新規遺伝マーカーを用いた網羅的クローンライブラリー法を開発した。
開発した手法を用いた結果,麻痺性貝毒発生海域の海水中における本藻類の動態ならびに詳細な発生種の把握(個体群解析)が可能であった。
さらに,有毒・有害プラントンの多くが休眠細胞となって沈降する底泥サンプルの培養ならびにクローンライブラリー法による実験結果から,春期における渦鞭毛藻のブルーム発生の予察もできる可能性が示唆された。

Research Products

• [Journal Article] Significant yield of ectoine by Halomonas sp. EG6 during osmotic downshock treatment, Diamino Amino Acid Research (2008)
  • Author(s): Osman O., Wang Y.Q., Zhu D., Hosoi-Tanabe S. and Nagata S
  • Journal Title: Nova Science Publishers
  • Peer Reviewed
• [Journal Article] Characterization of microorgamisms in Antarctic lakes. OCEANS'08 MTS/IEEE KOBE-TECHNO-OCEAN (2008)
  • Author(s): Zhang H., Hosoi-Tanabe S., Nagata S., Ban S. and Imura S
  • Journal Title: Proceedings in CD.
  • Peer Reviewed
• [Journal Article] Application of real-time PCR assay for detection and quantification of Alexandrium tamarense and Alexandrium catenella cysts from marine sediments (2007)
  • Author(s): Kamikawa R., Nagai S., Hosoi-Tanabe S., Itakura S., Yamaguchi M., Uchida Y., Baba,T. and Sako Y
  • Journal Title: Harmful Algae 6 Pages: 413-420
  • Peer Reviewed
• [Journal Article] Taurine transporter from the giant Pacific oyster: function and expression in response to hyper- and hypo-osmic stress (2007)
  • Author(s): Hosoi M.,Shinzato C. Takagi M. Hosoi-Tanabe S., Sawada H, Terasawa E. and Toyohara
  • Journal Title: Fish. Sci 73 Pages: 385-394
  • Peer Reviewed
• [Journal Article] Degradation of seaweed Wakame (Undaria pinnatifida) by composting process with inoculation of Bacillus sp. HR6 (2007)
  • Author(s): Tang J. C., Wei J. H., Maeda K., Kawai H., Zhou Q.,Hosoi-Tanabe S. and Nagata S
  • Journal Title: Biocontrol Science 12 Pages: 47-54
  • Peer Reviewed
• [Journal Article] Development and application of fluorescence in situ hybridization (FISH) method for simple and rapid identification of the toxic dinoflagellates Alexandrium tamarense and Alexandrium catenella in culture and natural seawater (2006)
  • Author(s): Hosoi-Tanabe S. and Yoshihiko Sako
  • Journal Title: Fish. Sci 72 Pages: 77-82
  • Peer Reviewed
• [Journal Article] Genetic differentiation in the marine dinoflagellates Alexandrium tamarense and Alexandrium catenella based on DNA-DNA hybridization (2006)
  • Author(s): Hosoi-Tanabe S. and Yoshihiko Sako
  • Journal Title: Plankton Benthos Res 1 Pages: 138-146
  • Peer Reviewed
• [Journal Article] Phylogenetic analysis of noxious red tide flagellates Chattonella antiqua, C. marina, C. ovata, and C. verruculosa (Raphidophyceae) based on the rRNA gene family (2006)
  • Author(s): Hosoi-Tanabe S., Otake I and Sako Y
  • Journal Title: Fish. Sci 72 Pages: 1200-1208
  • Peer Reviewed
• [Journal Article] 生物学的手法を用いたアオコ除去の可能性-琵琶湖の浄化を目指して (2006)
  • Author(s): 田辺祥子
  • Journal Title: 水 Vol.48-7 Pages: 14-19
• [Journal Article] 有害・有毒微細藻の分子モニタリング法の開発 (2006)
  • Author(s): 神川龍馬・田辺(細井)祥子・左子芳彦.
  • Journal Title: BRAIN TECHNO NEWS Vol.117 Pages: 28-31
• [Journal Article] Proposal of Pseudochattonella verruculasa gen. Nov., comb. Nov. (Dictyochophyceae) for a formar raphidophycean alga Chattonella verruculosa, based on 18S rDNA phylogeny and ultrastructural characteristics
  • Author(s): Hosoi-Tanabe S., Honda D., Fukaya S., Otake I., Inagaki Y. and Sako Y
  • Journal Title: Phycol. Res 55 Pages: 185-192
  • Peer Reviewed
• [Journal Article] Development of a novel molecular marker on the mitochondrial genome of a toxic dinoflagellate Alexandrium spp. And its application in single-cell PCR
  • Author(s): Kamikawa R.,Hosoi-Tanabe S., Yoshimatsu S., Oyama K., Masuda I. And Sako Y
  • Journal Title: J. Appl. Phycolol. J. Appl. Phycolol 20 Pages: 153-159
  • Peer Reviewed
• [Presentation] 海洋生物由来成分を用いた凝集沈澱によるバラスト水中の有害・有毒プランクトン除去の試み (2009)
  • Author(s): 田辺祥子・豊原治彦・大橋友隆
  • Organizer: 平成21年日本水産学会春季春季大会
  • Place of Presentation: 東京
  • Year and Date: 20090327-31
• [Presentation] 水産養殖用稚魚を介した中国からの有毒・有害プランクトンの越境移入の可能性 (2009)
  • Author(s): 田辺 祥子・細井 公富・伴 修平・朴 虎東・李 洪武
  • Organizer: 第33回日本藻類学会大会
  • Place of Presentation: 沖縄
  • Year and Date: 20090325-29
• [Presentation] Anaysis of community strucure of eukaryotic in the Aarctic lake sediment (2008)
  • Author(s): Zhang H., Hosoi-Tanabe S., Nagata S. and Hoshino T
  • Organizer: Polar Biology Symposium of the National Institute of Polar Research
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20081100
• [Presentation] cDNA cloning and mRNA expression analysis of cuticle proteins in Daphnia magna. ASLO meeting 2008 summer (2008)
  • Author(s): Hosoi M., Hosoi-Tanabe S., Sarai N., Ichii R., Ban S. and Mori T
  • Organizer: St. John's, Newfoundland
  • Place of Presentation: Canada
  • Year and Date: 20080608-13
• [Presentation] Molt cycle related vitellogenin mRNA _expression in daphnia magna. ASLO meeting, 2008 summer (2008)
  • Author(s): Ichi R, Hosoi M, Hosoi-Tanabe S, Sarai N, Mori T, Ban S
  • Organizer: St. John's, Newfoundland
  • Place of Presentation: Canada
  • Year and Date: 20080608-13
• [Presentation] Characterization of microorgamisms in Antarctic lakes (2008)
  • Author(s): Zhang H., Hosoi-Tanabe S., Nagata S., Ban S. and Imura S
  • Organizer: OCEANS'08 MTS/IEEE KOBE-TECHNO-OCEAN
  • Place of Presentation: Kobe, Japan,
  • Year and Date: 20080408-11
• [Presentation] Cultivation of microbial community in Antarctic lakes and phylogenetic analysis based on 16S rRNA gene sequences (2007)
  • Author(s): Zhang H., Hosoi-Tanabe S., Nagata, S., Ban S., Imura S
  • Organizer: Polar Biology Symposium of the National Institute of Polar Research
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20071115-16
• [Presentation] Horizontal distribution of Uroglena Americana resting cysts in bottom sediment of lake Biwa (2007)
  • Author(s): Ban, S., Yasuoka, H., Tagami, T., Hosoi-Tanabe, S., Ishikawa, K
  • Organizer: Japan. Societas Internationalis Limnologiae
  • Place of Presentation: Montreal, Canada
  • Year and Date: 20070800
• [Presentation] Distribution and diversity of planktonic microalgae in the coastal area and fireshwater lake in south China (Hainan Island) (2007)
  • Author(s): Hosoi Tanabe, S., Park, H-D., , H., Ban, S
  • Organizer: 21st Pacific Science Congress (PSC21)
  • Place of Presentation: Okinawa, Japan
  • Year and Date: 20070612-18
• [Presentation] オオミジンコ(Daphnia magna)におけるこみ合い応答遺伝子の発現解析 (2007)
  • Author(s): 更井紀一・市井涼子・田辺(細井)祥子・森司・伴修平
  • Organizer: 日本生態学会
  • Place of Presentation: 愛媛
  • Year and Date: 20070319-23
• [Presentation] Application of real-time PCR for monitoring a freshwater red-tide agent Uroglena americana (Chrysophyceaez) (2007)
  • Author(s): Ishikawa K. and Hosoi-Tanabe S., and Ban S
  • Organizer: ASLO 2007 Aquatic Sciences Meeting
  • Place of Presentation: USA
  • Year and Date: 20070204-9
• [Presentation] 中国海南省からの有毒・有害プランクトン移入の可能性 (2007)
  • Author(s): 田辺祥子・朴虎東・李洪武・伴修平
  • Organizer: 平成19年度日本水産学会秋季大会
  • Place of Presentation: 北海道
  • Year and Date: 20070025-28
• [Presentation] 分子生物学的手法による有毒アオコの動態解析 (2007)
  • Author(s): 田辺祥子
  • Organizer: 平成19年度日本水産学会近畿支部前期例会シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2007-07-07
• [Presentation] Application of real-time PCR assay to detection and quantification of Alexandrium tamarense and Alexandrium catenella cysts from marine sediments (2006)
  • Author(s): Kamikawa R., Nagai S., Hosoi-Tanabe S., Itakura S., Yamaguchi M., Uchida Y., Baba T. and Sako Y
  • Organizer: 3rd International workshop on targetd HAB species in the east Asia waters
  • Place of Presentation: Nagasaki, Japan
  • Year and Date: 20061117-19
• [Presentation] Molecular analysis of microbial diversity and temperature adaptability in Antarctic Lake. Polar Biology (2006)
  • Author(s): Zhang H., Hosoi-Tanabe S., Ban S., Imura S., Kudo S. and Matsuzaki M
  • Organizer: Symposium of the National Institute of Polar Research
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20061100
• [Presentation] Temperature adaptability and characteristics of microbial community in Antarctic oligohaline lake (2006)
  • Author(s): Zhang H., Hosoi-Tanabe S.,, Nagata S., Ban S., Imura S., Kudo S. and Matsuzaki, M
  • Organizer: Techno-Ocean 2006/19th JASNAOE Ocean Engineering Symposium
  • Place of Presentation: Kobe, Japan
  • Year and Date: 20061018-20
• [Presentation] 琵琶湖北湖堆積物からのUroglena americanaシストの収集とreal-time PCRによる定量法の検討 (2006)
  • Author(s): 伴修平,田上琢自,田辺(細井)祥子,石川可奈子
  • Organizer: 日本陸水学会第71回大会
  • Place of Presentation: 愛媛
  • Year and Date: 20060915-18
• [Presentation] 細菌における塩分ストレス耐性と増殖能に関する一考察 (2006)
  • Author(s): 村田綾子,石田昭夫,大島朗伸,佐々木秀明,田辺祥子,永田進一
  • Organizer: 第43回好塩微生物研究会
  • Place of Presentation: 奈良
  • Year and Date: 2006-12-17
• [Presentation] 好アルカリ性 Bacillus U-21 株を用いた補償溶質エクトインの生産方法について (2006)
  • Author(s): 大澤飛鳥,田辺祥子,永田進一,大島朗伸
  • Organizer: 第43回好塩微生物研究会
  • Place of Presentation: 奈良
  • Year and Date: 2006-12-17
• [Presentation] クローンライブラリー法による南極昭和基地
  • Author(s): 田辺祥子・伴修平・木村成子・工藤栄・伊村智・松崎雅広
• [Book] 貝毒原因有毒プランクトンの分子モニタリング. 水産学シリーズ153「貝毒研究の最前線(今井一郎・福代康夫・広石伸互編) (2007)
  • Author(s): 田辺(細井)祥子・神川龍馬・左子芳彦
  • Total Pages: 54-62
  • Publisher: 恒星社厚生閣
• [Remarks]
  • URL: http://www.research.kobe-u.ac.jp/rcis-kurcis/
• [Remarks]
  • URL: http://www.research.kobe-u.ac.jp/rcis-ecobio/