Signaling mechanisms inducing bacterial evolution

2008 Fiscal Year Final Research Report

• Project/Area Number: 18790301
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: University of Tsukuba
• Principal Investigator: MORIKAWA Kazuya University of Tsukuba, 大学院人間総合科学研究科, 准教授 (90361328)
• Project Period (FY): 2006 – 2008
• Keywords: 遺伝子 / 環境応答 / 細菌 / シグナル伝達 / ブドウ球菌 / 進化

Research Abstract

黄色ブドウ球菌において、DNA取り込み装置の発現条件は全く未知であった。本研究ではそれらの発現を直接制御するシグマ因子SigHの活性を詳細に調べることで、「SigHは細胞集団のごく一部でのみ活性化される」という事実を見出した。さらにそのメカニズムとして、独立した2つ、すなわち、1)sigH領域の重複による新規融合遺伝子の生成による活性化機構、および2)特定の培養条件で誘導される偶発的活性化、を見出し、さらにそれらの頻度解析や実際の分子機構の幾つかを明らかにした。

Research Products

• [Journal Article] Nucleoid Architecture Dynamics in bacteria (2009)
  • Author(s): Ryosuke L, Ohniwa, Kazuya Morikawa, Chieko Wada, Tkoshiko Ohta, and Kunio Takeyasu.
  • Journal Title: Bacterial DNA Research Progress (in press)
• [Journal Article] The analysis on different SigB concentration in Staphylococcus aureus 〜High SigB accumulation enhances biofilm formation〜 (2009)
  • Author(s): Sayaka L. Takeshita, Toshie Hidaka, Toshiko Ohta, and Kazuya Morikawa.
  • Journal Title: Current Research Topics in Applied Microbiology and Microbial Biotechnology(Antonio Mendez-Vilas, World Scientific Publishing Co. Pte. Ltd.) Pages: 611-614
• [Journal Article] The sigH gene sequence can subspeciate staphylococci. (2008)
  • Author(s): Kazuya Morikawa, Ryosuke L. Ohniwa, Miyuki Kumano, Hideyuki Okamura, Shinji Saito, Toshiko Ohta.
  • Journal Title: Diagnostic Microbiology and Infectious Disease 61 Pages: 373-380
  • URL: http://hdl.handle.net/2241/100343
  • Peer Reviewed
• [Journal Article] Transcription-coupled Nucleoid Architecture in Bacteria. (2007)
  • Author(s): Ryosuke L. Ohniwa, Kazuya Morikawa, Sayaka L. Takeshita, Joongbaek Kim, Toshiko Ohta, Chieko Wada, and Kunio Takeyasu.
  • Journal Title: Genes Cells 12 Pages: 1141-1152
  • Peer Reviewed
• [Journal Article] Structural and Mutational analyses of Drp35 from Staphylococcus aureus : a possible mechanism for its lactonase activity. (2007)
  • Author(s): Yoshikazu Tanaka, Kazuya Morikawa, Yu Ohki, Min Yao, Kouhei Tsumoto, Nobuhisa Watanabe, Toshiko Ohta, and Isao Tanaka.
  • Journal Title: J. Biol. Chem. 282 Pages: 5770-5780
  • Peer Reviewed
• [Journal Article] Biochemical, Molecular Genetic, and Structural Analyses of the Staphylococcal Nucleoid. (2007)
  • Author(s): Kazuya Morikawa, Ryosuke L. Ohniwa, Joongbaek Kim, Sayaka L. Takeshita, Atsushi Maruyama, Yumiko Inose, Kunio Takeyasu, and Toshiko Ohta.
  • Journal Title: Microscopy and Microanalysis 13 Pages: 30-35
  • Peer Reviewed
• [Journal Article] Atomic Force Microscopy Dissects the Hierarchy of Genome Architectures in Eukaryote, Prokaryote and Chloroplast. (2007)
  • Author(s): Ryosuke L. Ohniwa, Kazuya Morikawa, Joongbaek Kim, T. Kobori, K. Hizume, R. Matsumi, H. Atomi, T. Imanaka, Toshiko Ohta, Shige H. Yoshimura, and Kunio Takeyasu.
  • Journal Title: Microscopy and Microanalysis 13 Pages: 3-12
  • Peer Reviewed
• [Journal Article] Genetic characterization of the natural SigB variants found in clinical isolates of Staphylococcus aureus. (2006)
  • Author(s): Yumiko Inose, Sayaka L. Takeshita, Toshie Hidaka, Masato Higashide, Atsushi Mauyama, Hideo Hayashi, Kazuya Morikawa, and Toshiko Ohta.
  • Journal Title: J. Gen. Appl. Microbiol. 52(5) Pages: 259-271
  • Peer Reviewed
• [Journal Article] Dynamic state of DNA topology is essential for genome condensation in bacteria. (2006)
  • Author(s): Ryosuke L. Ohniwa, Kazuya Morikawa, Joongbaek Kim, Toshiko Ohta, Akira Ishihama, Chieko Wada and Kunio Takeyasu.
  • Journal Title: EMBO J 25 Pages: 5591-5602
  • Peer Reviewed
• [Journal Article] Bacterial Nucleoid Dynamics : Oxidative Stress Response in Staphylococcus aureus. (2006)
  • Author(s): Kazuya Morikawa, Ryosuke L. Ohniwa, Joongbaek Kim, Atsushi Maruyama, Toshiko Ohta and Kunio Takeyasu.
  • Journal Title: Genes Cells 11 Pages: 409-423
  • Peer Reviewed
• [Presentation] Nucleoid architecture in bacteria revealed by atomic force microscopy Society of General (2008)
  • Author(s): Kunio Takeyasu, Akira Shiraishi, Hirohide Takahashi, Kohji Hizue Kazuya Morikawa, Ryosuke L. Ohniwa, and Chieko Wada
  • Organizer: Microbiology 163rd Meeting
  • Place of Presentation: Trinity College Dublin, Ireland
  • Year and Date: 2008-09-11
• [Presentation] Contribution of cardiolipin synthase to the salt resistance (2008)
  • Author(s): Meng-Ju Melody Tsai, Kazuya Morikawa, Ryosuke Ohniwa, Toshiko Ohta, Shinji Saito, Hideo Hayashi.
  • Organizer: 13th International Symposium on Staphylococci and Staphylococcal Infections
  • Place of Presentation: Cairns Convention Centre, Australia
  • Year and Date: 2008-09-07
• [Presentation] Quantitative analysis of sigma factors in Staphylococcus aureus. (2007)
  • Author(s): Sayaka L. Takeshita, Toshiko Ohta and Kazuya Morikawa
  • Organizer: 2nd International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld-2007)
  • Place of Presentation: Seville, Spain
  • Year and Date: 2007-11-28
• [Presentation] A New Staphylococcal Sigma Factor, SigH, Regulates Competence Gene Homologues. (2007)
  • Author(s): Kazuya Morikawa, Sayaka L. Takeshita, Yumiko Inose, Tarek Msadek and Toshiko Ohta.
  • Organizer: 4th Conference on Functional Genomics of Gram-Positive Microorganisms.
  • Place of Presentation: Tirrenia, Pisa, Italy
  • Year and Date: 2007-06-24
• [Presentation] Bacterial stress response 〜sigma factors in Staphylococcus aures〜 (2006)
  • Author(s): Sayaka L. Takeshita, Kazuya Morikawa, and Toshiko Ohta.
  • Organizer: The 8th AEARU joint workshop on Life Science
  • Place of Presentation: つくば
  • Year and Date: 20061104-06
• [Presentation] RNA-mediated Nucleoid Architecture in Bacteria. (2006)
  • Author(s): Ryosuke L. Ohniwa, Kazuya Morikawa, Joongbaek Kim, Toshko Ohta, Chieko Wada and Kunio Takeyasu.
  • Organizer: 第46回米国細胞生物学会大会
  • Place of Presentation: 米国、サンディエゴ
  • Year and Date: 2006-12-09
• [Presentation] Direct Measurement of Interactions between SigB and RsbW Proteins (2006)
  • Author(s): 中田良樹、山口隆広、鈴木武博、森川一也、栗原和枝
  • Organizer: 第44回生物物理学会年会及び第5回東アジア生物物理学シンポジウム
  • Place of Presentation: 那覇
  • Year and Date: 2006-11-12
• [Presentation] Crystal structural analyses of virulence factors from Staphylococcus aureus. (2006)
  • Author(s): Yoshikazu Tanaka, Yu Ohki, Ayuko Suenaga, Kazuya Morikawa, Makoto Kuroda, Min Yao, Kouhei Tsumoto, Nobuhisa Watanabe, Izumi Kumagai, Toshiko Ohta, and Isao Tanaka
  • Organizer: International Conference on Structural Genomics 2006 (ICSG2006)
  • Place of Presentation: 北京
  • Year and Date: 2006-10-22
• [Presentation] RNA-mediated Nucleoid Architecture in Bacteria. (2006)
  • Author(s): Ryosuke L. Ohniwa, Kazuya Morikawa, Joongbaek Kim, Toshiko Ohta, Chieko Wada and Kunio Takeyasu
  • Organizer: Molecular Genetics of Bacteria and Phages meeting
  • Place of Presentation: CSHL 米国
  • Year and Date: 2006-08-22
• [Presentation] Dynamic state of DNA topology is essential for genome condensation in bacteria. (2006)
  • Author(s): Kunio Takeyasu, Ryosuke L. Ohniwa, Kazuya Morikawa, Joongbaek Kim, Toshiko Ohta, Akira Ishihama and Chieko Wada.
  • Organizer: Molecular Genetics of Bacteria and Phages meeting
  • Place of Presentation: CSHL 米国
  • Year and Date: 2006-08-22
• [Presentation] Structural and mutational analyses of Drp35, a 35kDa drug responsive protein from Staphylococcus aureus. (2006)
  • Author(s): Yoshikazu Tanaka, Kazuya Morikawa, Masaru Ohki, Min Yao, Nobuhisa Watanabe, Toshiko Ohta and Isao Tanaka
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto Japan
  • Year and Date: 2006-06-18
• [Presentation] Gene Diversity of Staphylococcal SigH 〜application for species identification〜 (2006)
  • Author(s): Yu Ohki, Miyuki Kumano, Hideyuki Okamura, Yumiko Inose, Kazuya Morikawa, Toshiko Ohta
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto Japan
  • Year and Date: 2006-06-18
• [Presentation] Genetic characterization of the natural SigB variants found in clinical isolates of Staphylococcus aureus (2006)
  • Author(s): Sayaka. L. Takeshita, Kazuya Morikawa, Yumiko Inose, Toshie Hidaka, Masato Higashide, Toshiko Ohta
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Kyoto Japan
  • Year and Date: 2006-06-18
• [Book] 「メディカルサイエンス微生物検査学」大学検査科学専攻微生物学教員懇談会編, 241-246 (2008)
  • Publisher: 近代出版
• [Book] ヒトの生物学(永田恭介監訳)15章:ヒトの感染症, 316-339 (2007)
  • Publisher: 丸善書店(翻訳分担)
• [Remarks] ホームページ
  • URL: http://www.md.tsukuba.ac.jp/basic-med/infectionbiology/microbiology/index.htm